Synthetic methylotrophs
Abstract
The present invention provides a non-naturally occurring microbe capable of growing in a medium comprising methanol, comprising a heterologous polynucleotide encoding a heterologous methanol dehydrogenase (MDH) derived from a Corynebacterium organism (Cor), wherein the MDH is expressed in the microbe, and wherein the MDH exhibits a Km of no more than 3 mM for methanol. Also provided are uses of the non-naturally occurring microbe for oxidizing methanol and producing a metabolite as well as the preparation of the non-naturally occurring microbe.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Delaware, Newark, DE (United States)
- Sponsoring Org.:
- USDOE Advanced Research Projects Agency - Energy (ARPA-E)
- OSTI Identifier:
- 1650848
- Patent Number(s):
- 10640746
- Application Number:
- 16/070,318
- Assignee:
- University of Delaware (Newark, DE)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
- DOE Contract Number:
- AR0000432
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 01/13/2017
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 09 BIOMASS FUELS
Citation Formats
Papoutsakis, Eleftherios T., Whitaker, William Brian, and Bennett, Robert Kyle. Synthetic methylotrophs. United States: N. p., 2020.
Web.
Papoutsakis, Eleftherios T., Whitaker, William Brian, & Bennett, Robert Kyle. Synthetic methylotrophs. United States.
Papoutsakis, Eleftherios T., Whitaker, William Brian, and Bennett, Robert Kyle. Tue .
"Synthetic methylotrophs". United States. https://www.osti.gov/servlets/purl/1650848.
@article{osti_1650848,
title = {Synthetic methylotrophs},
author = {Papoutsakis, Eleftherios T. and Whitaker, William Brian and Bennett, Robert Kyle},
abstractNote = {The present invention provides a non-naturally occurring microbe capable of growing in a medium comprising methanol, comprising a heterologous polynucleotide encoding a heterologous methanol dehydrogenase (MDH) derived from a Corynebacterium organism (Cor), wherein the MDH is expressed in the microbe, and wherein the MDH exhibits a Km of no more than 3 mM for methanol. Also provided are uses of the non-naturally occurring microbe for oxidizing methanol and producing a metabolite as well as the preparation of the non-naturally occurring microbe.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 05 00:00:00 EDT 2020},
month = {Tue May 05 00:00:00 EDT 2020}
}
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Works referenced in this record:
Coryneform Bacterium Transformant and Process for Producing Dicarboxylic Acids Using the Same
patent-application, April 2007
- Murakami, Shikiko; Nakata, Kaori; Okino, Shohei
- US Patent Application 10/566129; 20070087423
Method for Producing Target Substance
patent-application, September 2004
- Takeshita, Ryo; Yasueda, Hisashi
- US Patent Application 10/792647; 20040191875