Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components
Abstract
Raman spectra of cells, such as normal human T- and B-cells from peripheral blood or human tonsil and the corresponding transformed cells are obtained by optically trapping the cells and obtaining their Raman spectra. The trapped cells can be subjected to one, two, or more different excitation wavelengths, and each wavelength of the corresponding Raman spectra can be stored in a separate channel. In preferred embodiments, two spectra are subtracted from each other to give a difference spectrum and each channel is analyzed independently to characterize the trapped cell. Alternatively, the Raman spectrum can be subjected to Principal Component Analysis (PCA) in order to characterize the trapped cell. The trapped cell thus classified can be sorted, or further manipulated.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568650
- Patent Number(s):
- 10365220
- Application Number:
- 11/916,273
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- PHY-012099; W-7405-ENG-48
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 05/31/2006
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Chan, James W., Huser, Thomas R., Lane, Stephen M., Rutledge, John C., Taylor, Douglas S., Zwerdling, Theodore, and Li, Ronald. Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components. United States: N. p., 2019.
Web.
Chan, James W., Huser, Thomas R., Lane, Stephen M., Rutledge, John C., Taylor, Douglas S., Zwerdling, Theodore, & Li, Ronald. Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components. United States.
Chan, James W., Huser, Thomas R., Lane, Stephen M., Rutledge, John C., Taylor, Douglas S., Zwerdling, Theodore, and Li, Ronald. Tue .
"Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components". United States. https://www.osti.gov/servlets/purl/1568650.
@article{osti_1568650,
title = {Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components},
author = {Chan, James W. and Huser, Thomas R. and Lane, Stephen M. and Rutledge, John C. and Taylor, Douglas S. and Zwerdling, Theodore and Li, Ronald},
abstractNote = {Raman spectra of cells, such as normal human T- and B-cells from peripheral blood or human tonsil and the corresponding transformed cells are obtained by optically trapping the cells and obtaining their Raman spectra. The trapped cells can be subjected to one, two, or more different excitation wavelengths, and each wavelength of the corresponding Raman spectra can be stored in a separate channel. In preferred embodiments, two spectra are subtracted from each other to give a difference spectrum and each channel is analyzed independently to characterize the trapped cell. Alternatively, the Raman spectrum can be subjected to Principal Component Analysis (PCA) in order to characterize the trapped cell. The trapped cell thus classified can be sorted, or further manipulated.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 30 00:00:00 EDT 2019},
month = {Tue Jul 30 00:00:00 EDT 2019}
}
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