Installation of genomes or partial genomes into cells or cell-like systems
Abstract
A method is provided for introducing a genome into a cell or cell-like system. The introduced genome may occur in nature, be manmade with or without automation, or may be a hybrid of naturally occurring and manmade materials. The genome is obtained outside of a cell with minimal damage. Materials such as a proteins, RNAs, polycations, nucleoid condensation proteins, or gene translation systems may accompany the genome. The genome is installed into a naturally occurring cell or into a manmade cell-like system. A cell-like system or synthetic cell resulting from the practice of the provided method may be designed and used to yield gene-expression products, such as desired proteins. By enabling the synthesis of cells or cell-like systems comprising a wide variety of genomes, accompanying materials and membrane types, the provided method makes possible a broader field of experimentation and bioengineering than has been available using prior art methods.
- Inventors:
- Issue Date:
- Research Org.:
- Synthetic Genomics, Inc., San Diego, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1531941
- Patent Number(s):
- 9434974
- Application Number:
- 11/644,713
- Assignee:
- Synthetic Genomics, Inc. (San Diego, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG02-02ER63453
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2006-12-22
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Glass, John I., Young, Lei, Lartigue, Carole, Assad-Garcia, Nacyra, Smith, Hamilton O., Hutchison, Clyde A., and Venter, J. Craig. Installation of genomes or partial genomes into cells or cell-like systems. United States: N. p., 2016.
Web.
Glass, John I., Young, Lei, Lartigue, Carole, Assad-Garcia, Nacyra, Smith, Hamilton O., Hutchison, Clyde A., & Venter, J. Craig. Installation of genomes or partial genomes into cells or cell-like systems. United States.
Glass, John I., Young, Lei, Lartigue, Carole, Assad-Garcia, Nacyra, Smith, Hamilton O., Hutchison, Clyde A., and Venter, J. Craig. Tue .
"Installation of genomes or partial genomes into cells or cell-like systems". United States. https://www.osti.gov/servlets/purl/1531941.
@article{osti_1531941,
title = {Installation of genomes or partial genomes into cells or cell-like systems},
author = {Glass, John I. and Young, Lei and Lartigue, Carole and Assad-Garcia, Nacyra and Smith, Hamilton O. and Hutchison, Clyde A. and Venter, J. Craig},
abstractNote = {A method is provided for introducing a genome into a cell or cell-like system. The introduced genome may occur in nature, be manmade with or without automation, or may be a hybrid of naturally occurring and manmade materials. The genome is obtained outside of a cell with minimal damage. Materials such as a proteins, RNAs, polycations, nucleoid condensation proteins, or gene translation systems may accompany the genome. The genome is installed into a naturally occurring cell or into a manmade cell-like system. A cell-like system or synthetic cell resulting from the practice of the provided method may be designed and used to yield gene-expression products, such as desired proteins. By enabling the synthesis of cells or cell-like systems comprising a wide variety of genomes, accompanying materials and membrane types, the provided method makes possible a broader field of experimentation and bioengineering than has been available using prior art methods.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 06 00:00:00 EDT 2016},
month = {Tue Sep 06 00:00:00 EDT 2016}
}
Works referenced in this record:
Method for the complete chemical synthesis and assembly of genes and genomes
patent, February 2003
- Evans, Glen A.
- US Patent Document 6,521,427