Title: Nanochannel arrays and their preparation and use for high throughput macromolecular analysis

Abstract

Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.

Inventors:

Austin, Robert H.; Yu, Zhaoning; Tegenfeldt, Jonas O.; Chou, Stephen Y.; Cao, Han

Issue Date:

Tue Jul 12 00:00:00 EDT 2016

Research Org.:

Princeton Univ., NY (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1531921

Patent Number(s):

9389217

Application Number:

14/223,589

Assignee:

The Trustees of Princeton University (Princeton, NJ)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J2219/00274 - Sequential or parallel reactions

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2200/0663 - Stretching or orienting elongated molecules or particles
B01L2200/12 - Specific details about manufacturing devices
B01L2300/0627 - Sensor or part of a sensor is integrated
B01L2300/0861 - Configuration of multiple channels and/or chambers in a single devices
B01L2300/0896 - Nanoscaled
B01L2400/0415 - electrical forces, e.g. electrokinetic
B01L2400/0418 - electro-osmotic flow [EOF]
B01L2400/086 - using baffles or other fixed flow obstructions
B01L3/502707 - {characterised by the manufacture of the container or its components

B - PERFORMING OPERATIONS B81 - MICROSTRUCTURAL TECHNOLOGY B81B - MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES

B81B1/002 - {Holes characterised by their shape, in either longitudinal or sectional plane}
B81B2201/058 - Microfluidics not provided for in B81B2201/051 - B81B2201/054
B81B2203/0338 - Channels

B - PERFORMING OPERATIONS B81 - MICROSTRUCTURAL TECHNOLOGY B81C - PROCESSES OR APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OR TREATMENT OF MICROSTRUCTURAL DEVICES OR SYSTEMS

B81C1/00071 - {Channels}

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

B82Y30/00 - Nanotechnology for materials or surface science, e.g. nanocomposites

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6837 - using probe arrays or probe chips
C12Q1/6869 - Methods for sequencing

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N21/6428 - {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
G01N21/648 - {using evanescent coupling or surface plasmon coupling for the excitation of fluorescence}
G01N27/447 - using electrophoresis {
G01N27/44791 - {Microapparatus
G01N33/48721 - {Investigating individual macromolecules, e.g. by translocation through nanopores

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S977/704 - Nucleic acids, e.g. DNA or RNA
Y10S977/88 - with arrangement, process, or apparatus for testing
Y10S977/883 - Fluidic self-assembly, FSA

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Resource Type:

Patent

Resource Relation:

Patent File Date: 2014-03-24

Country of Publication:

United States

Language:

English