Column device for isolation and labeling of nucleic acids
Abstract
A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Chicago, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1531485
- Patent Number(s):
- 6818398
- Application Number:
- 09/751,654
- Assignee:
- The University of Chicago (Chicago, IL)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
- DOE Contract Number:
- W-31-109-ENG-38
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2000-12-29
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., and Mirzabekov, Andrei. Column device for isolation and labeling of nucleic acids. United States: N. p., 2004.
Web.
Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., & Mirzabekov, Andrei. Column device for isolation and labeling of nucleic acids. United States.
Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., and Mirzabekov, Andrei. Tue .
"Column device for isolation and labeling of nucleic acids". United States. https://www.osti.gov/servlets/purl/1531485.
@article{osti_1531485,
title = {Column device for isolation and labeling of nucleic acids},
author = {Bavykin, Sergei G. and Akowski, James P. and Zakhariev, Vladimir M. and Mirzabekov, Andrei},
abstractNote = {A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 16 00:00:00 EST 2004},
month = {Tue Nov 16 00:00:00 EST 2004}
}
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Works referencing / citing this record:
Method for labeling DNA and RNA
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- Bavykin, Sergei; Mirzabekov, Andrei Darievich
- US Patent Document 7,208,269
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- Laurent, Alain; Laayoun, Ali; Kotera, Mitsuhara
- US Patent Document 9,266,902
Labeling reagents, methods for the synthesis of such reagents and methods for the detection of biological molecules
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- Laayoun, Ali; Bernal-Mendez, Eloy
- US Patent Document 7,691,635
Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling
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- Bourget, Cecile; Kotera, Mitsuharu; Lhomme, Jean
- US Patent Document 7,060,441
Marking reagents bearing diazo and nitro functions, methods for the synthesis of such reagents and methods for detecting biological molecules
patent, November 2012
- Laurent, Alain; Laayoun, Ali; Kotera, Mitsuharu
- US Patent Document 8,309,695
Universal nucleic acids sample preparation method for cells, spores and their mixture
patent, January 2011
- Bavykin, Sergei
- US Patent Document 7,871,764