SecA gene of mycobacterium tuberculosis and related methods and compositions
Abstract
An isolated nucleic acid encoding a SecA protein of M. tuberculosis is provided. This nucleic acid can be a native coding sequence for the SecA protein of M. tuberculosis. A specific example of the isolated nucleic acid is one that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2. An isolated fragment of the secA gene that is specific for M. tuberculosis is provided. A purified SecA protein of Mycobacterium tuberculosis is provided. The purified SecA protein of Mycobacterium tuberculosis comprises the polypeptide having the sequence set forth in the Sequence Listing as SEQ ID NO:2. Fragments of the M. tuberculosis SecA protein are provided. A purified mutant SecA protein of Mycobacterium tuberculosis is provided. The invention provides purified mutant M. tuberculosis expressing the mutant SecA protein of the invention. The invention also provides methods of screening for putative M. tuberculosis virulence factors translocated by the SecA protein.
- Inventors:
- Issue Date:
- Research Org.:
- The United States of America as represented by the Department of Health and Human Services, Washington, D.C. (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1531425
- Patent Number(s):
- 5885828
- Application Number:
- 08/801,105
- Assignee:
- The United States of America as represented by the Department of Health and Human Services (Washington, DC)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- FG01-912EW506
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 1997-02-14
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Quinn, Frederick D., Owens, Marie U., King, C. Harold, and Schmidt, Michael G. SecA gene of mycobacterium tuberculosis and related methods and compositions. United States: N. p., 1999.
Web.
Quinn, Frederick D., Owens, Marie U., King, C. Harold, & Schmidt, Michael G. SecA gene of mycobacterium tuberculosis and related methods and compositions. United States.
Quinn, Frederick D., Owens, Marie U., King, C. Harold, and Schmidt, Michael G. Tue .
"SecA gene of mycobacterium tuberculosis and related methods and compositions". United States. https://www.osti.gov/servlets/purl/1531425.
@article{osti_1531425,
title = {SecA gene of mycobacterium tuberculosis and related methods and compositions},
author = {Quinn, Frederick D. and Owens, Marie U. and King, C. Harold and Schmidt, Michael G.},
abstractNote = {An isolated nucleic acid encoding a SecA protein of M. tuberculosis is provided. This nucleic acid can be a native coding sequence for the SecA protein of M. tuberculosis. A specific example of the isolated nucleic acid is one that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2. An isolated fragment of the secA gene that is specific for M. tuberculosis is provided. A purified SecA protein of Mycobacterium tuberculosis is provided. The purified SecA protein of Mycobacterium tuberculosis comprises the polypeptide having the sequence set forth in the Sequence Listing as SEQ ID NO:2. Fragments of the M. tuberculosis SecA protein are provided. A purified mutant SecA protein of Mycobacterium tuberculosis is provided. The invention provides purified mutant M. tuberculosis expressing the mutant SecA protein of the invention. The invention also provides methods of screening for putative M. tuberculosis virulence factors translocated by the SecA protein.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 23 00:00:00 EST 1999},
month = {Tue Mar 23 00:00:00 EST 1999}
}
Works referenced in this record:
In vivo and in vitro characterization of the secA gene product of Bacillus subtilis.
journal, January 1992
- Takamatsu, H.; Fuma, S.; Nakamura, K.
- Journal of Bacteriology, Vol. 174, Issue 13