Title: Method of nucleic acid cassette assembly

Abstract

Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Rational methods may be used to design the synthetic genome (e.g., to establish a minimal genome and/or to optimize the function of genes within a genome, such as by mutating or rearranging the order of the genes). Synthetic genomes of the invention may be introduced into vesicles (e.g., bacterial cells from which part or all of the resident genome has been removed, or synthetic vesicles) to generate synthetic cells. Synthetic genomes or synthetic cells may be used for a variety of purposes, including the generation of synthetic fuels, such as hydrogen or ethanol.

Inventors:

Venter, J. Craig; Smith, Hamilton O.; Hutchison, III, Clyde A.; Gibson, Daniel G.

Issue Date:

Tue Aug 07 00:00:00 EDT 2018

Research Org.:

Synthetic Genomics, Inc., La Jolla, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1531354

Patent Number(s):

10041060

Application Number:

11/635,355

Assignee:

Synthetic Genomics, Inc. (La Jolla, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12N15/1093 - {General methods of preparing gene libraries, not provided for in other subgroups}
C12N15/66 - General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation

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DOE Contract Number:  

FG02-02ER63453

Resource Type:

Patent

Resource Relation:

Patent File Date: 2006-12-06

Country of Publication:

United States

Language:

English