Title: Discovery, detection and use of biomarkers

Abstract

Provided herein are systems for and methods of capturing, detecting, quantifying, and characterizing target moieties that are characterized by having a lipophilic portion of sufficient size and chemical composition whereby the target moiety inserts (or partitions) into a lipid assembly. Examples of such assays employ synthetic lipid constructs such as supported bilayers which are used to capture target moieties; other example assays exploit the natural absorption of compounds into natural lipid constructs such as HDL or LDL particles or cell membranes to capture target moieties. In specific embodiments, the target moieties are bacterial pathogen associated molecular pattern (PAMP) molecules or compounds not yet identified as PAMP molecules. Also provided are methods of determining PAMP molecule fingerprints and profiles that are linked to (indicative of) bacterial infection, disease states or progression, development of antibiotic resistance, and so forth, as well as these fingerprints, profiles and methods of using them.

Inventors:

Swanson, Basil I.; Mukundan, Harshini; Sakamuri, Rama Murthy

Issue Date:

Tue Dec 05 00:00:00 EST 2017

Research Org.:

Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1411405

Patent Number(s):

9835618

Application Number:

13/529,847

Assignee:

Los Alamos National Security, LLC (Los Alamos, NM)

Patent Classifications (CPCs):

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N21/7703 - {using reagent-clad optical fibres or optical waveguides
G01N2333/35 - from Mycobacteriaceae
G01N2400/50 - Lipopolysaccharides
G01N33/53 - Immunoassay
G01N33/5432 - {Liposomes or microcapsules}
G01N33/54373 - {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings}

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DOE Contract Number:  

AC52-06NA25396

Resource Type:

Patent

Resource Relation:

Patent File Date: 2012 Jun 21

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES