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Title: Hyperspectral imaging flow cytometer

Abstract

A hyperspectral imaging flow cytometer can acquire high-resolution hyperspectral images of particles, such as biological cells, flowing through a microfluidic system. The hyperspectral imaging flow cytometer can provide detailed spatial maps of multiple emitting species, cell morphology information, and state of health. An optimized system can image about 20 cells per second. The hyperspectral imaging flow cytometer enables many thousands of cells to be characterized in a single session.

Inventors:
;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1404931
Patent Number(s):
9797836
Application Number:
13/769,724
Assignee:
National Technology & Engineering Solutions of Sandia, LLC
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Feb 18
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Sinclair, Michael B., and Jones, Howland D. T. Hyperspectral imaging flow cytometer. United States: N. p., 2017. Web.
Sinclair, Michael B., & Jones, Howland D. T. Hyperspectral imaging flow cytometer. United States.
Sinclair, Michael B., and Jones, Howland D. T. Wed . "Hyperspectral imaging flow cytometer". United States. https://www.osti.gov/servlets/purl/1404931.
@article{osti_1404931,
title = {Hyperspectral imaging flow cytometer},
author = {Sinclair, Michael B. and Jones, Howland D. T.},
abstractNote = {A hyperspectral imaging flow cytometer can acquire high-resolution hyperspectral images of particles, such as biological cells, flowing through a microfluidic system. The hyperspectral imaging flow cytometer can provide detailed spatial maps of multiple emitting species, cell morphology information, and state of health. An optimized system can image about 20 cells per second. The hyperspectral imaging flow cytometer enables many thousands of cells to be characterized in a single session.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Oct 25 00:00:00 EDT 2017},
month = {Wed Oct 25 00:00:00 EDT 2017}
}

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  • Timlin, Jerilyn A.; Nieman, Linda T.; Jones, Howland D. T.
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Accurate Detection of Low Levels of Fluorescence Emission in Autofluorescent Background: Francisella-Infected Macrophage Cells
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Antimicrobial peptide interactions with silica bead supported bilayers and E. coli: buforin II, magainin II, and arenicin
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In vivo hyperspectral confocal fluorescence imaging to determine pigment localization and distribution in cyanobacterial cells
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Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution
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