Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

Church, George M.; Esvelt, Kevin; Mali, Prashant

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Title: Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

Abstract

Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.

Inventors:: Church, George M.; Esvelt, Kevin; Mali, Prashant

Issue Date:: Tue Mar 07 00:00:00 EST 2017

Research Org.:: Harvard Univ., Cambridge, MA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1346066

Patent Number(s):: 9587252

Application Number:: 14/674,895

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K2/00 - Peptides of undefined number of amino acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/11 - DNA or RNA fragments
C12N15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N15/85 - for animal cells
C12N15/907 - {in mammalian cells}
C12N2310/3519 - Fusion with another nucleic acid
C12N2320/33 - Alteration of splicing
C12N2320/50 - Methods for regulating/modulating their activity
C12N2800/107 - for mammalian
C12N2800/40 - Systems of functionally co-operating vectors
C12N2800/80 - Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
C12N2810/10 - Vectors comprising a non-peptidic targeting moiety
C12N2830/00 - Vector systems having a special element relevant for transcription
C12N2830/34 - being a transcription initiation element
C12N2999/007 - Technological advancements, e.g. new system for producing known virus, cre-lox system for production of transgenic animals
C12N9/22 - Ribonucleases {RNAses, DNAses

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y301/00 - Hydrolases acting on ester bonds

Show less

DOE Contract Number:: FG02-02ER63445

Resource Type:: Patent

Resource Relation:: Patent File Date: 2015 Mar 31

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Church, George M., Esvelt, Kevin, and Mali, Prashant. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.  United States: N. p., 2017. 
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                    Church, George M., Esvelt, Kevin, & Mali, Prashant. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.  United States.

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                    Church, George M., Esvelt, Kevin, and Mali, Prashant. Tue .  
        "Orthogonal Cas9 proteins for RNA-guided gene regulation and editing".  United States.  https://www.osti.gov/servlets/purl/1346066.

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@article{osti_1346066,

  title        = {Orthogonal Cas9 proteins for RNA-guided gene regulation and editing},

  author       = {Church, George M. and Esvelt, Kevin and Mali, Prashant},

  abstractNote = {Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Mar 07 00:00:00 EST 2017},

  month        = {Tue Mar 07 00:00:00 EST 2017}

}

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Similar Records in DOE Patents and OSTI.GOV collections:

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

Patent Church, George M.; Esvelt, Kevin M.; Mali, Prashant G.

Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.
Full Text Available
Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

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Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.
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Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein ormore » « less
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Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein ormore » « less
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Similar Records

Title: Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

Abstract

Citation Formats

Methods and compositions for treating Huntington's disease patent-application, September 2013

Mature clustered, regularly interspaced, short palindromic repeats RNA (crRNA) length is measured by a ruler mechanism anchored at the precursor processing site journal, December 2011

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity journal, June 2012

Diverse CRISPRs Evolving in Human Microbiomes journal, June 2012

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria journal, September 2012

Visualization of RNA in living cells patent, July 2003

A CRISPR Approach to Gene Targeting journal, September 2012

Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs journal, February 2012

CRISPR-Cas systems and methods for altering expression of gene products patent, April 2014

Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications patent-application, June 2014

Prokaryotic RNAi-Like System and Methods of Use patent-application, August 2011

Endroibonuclease Compositions and Methods of Use Thereof patent-application, May 2013

Target DNA interference with crRNA patent-application, March 2010

Novel DNA-binding Proteins And Uses Thereof patent-application, December 2011

Multiplex Genome Engineering Using CRISPR/Cas Systems journal, January 2013

Methods And Compositions For Rna-Directed Target Dna Modification And For Rna-Directed Modulation Of Transcription patent-application, March 2014

Clustered regularly interspaced short palindromic repeats (CRISPRs): the hallmark of an ingenious antiviral defense mechanism in prokaryotes journal, July 2011

Methods of Generating Nucleic Acid Fragments patent-application, September 2011

RNA-guided genetic silencing systems in bacteria and archaea journal, February 2012

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes journal, July 2013

RNA-programmed genome editing in human cells journal, January 2013

Evolution and classification of the CRISPR–Cas systems journal, May 2011

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression journal, February 2013

Methods and compositions for treating Huntington's disease
patent-application, September 2013

Mature clustered, regularly interspaced, short palindromic repeats RNA (crRNA) length is measured by a ruler mechanism anchored at the precursor processing site
journal, December 2011

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
journal, June 2012

Diverse CRISPRs Evolving in Human Microbiomes
journal, June 2012

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
journal, September 2012

Visualization of RNA in living cells
patent, July 2003

A CRISPR Approach to Gene Targeting
journal, September 2012

Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs
journal, February 2012

CRISPR-Cas systems and methods for altering expression of gene products
patent, April 2014

Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
patent-application, June 2014

Prokaryotic RNAi-Like System and Methods of Use
patent-application, August 2011

Endroibonuclease Compositions and Methods of Use Thereof
patent-application, May 2013

Target DNA interference with crRNA
patent-application, March 2010

Novel DNA-binding Proteins And Uses Thereof
patent-application, December 2011

Multiplex Genome Engineering Using CRISPR/Cas Systems
journal, January 2013

Methods And Compositions For Rna-Directed Target Dna Modification And For Rna-Directed Modulation Of Transcription
patent-application, March 2014

Clustered regularly interspaced short palindromic repeats (CRISPRs): the hallmark of an ingenious antiviral defense mechanism in prokaryotes
journal, July 2011

Methods of Generating Nucleic Acid Fragments
patent-application, September 2011

RNA-guided genetic silencing systems in bacteria and archaea
journal, February 2012

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
journal, July 2013

RNA-programmed genome editing in human cells
journal, January 2013

Evolution and classification of the CRISPR–Cas systems
journal, May 2011

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
journal, February 2013