Cellulase variants
Abstract
This invention provides novel variant cellulolytic enzymes having improved activity and/or stability. In certain embodiments the variant cellulotyic enzymes comprise a glycoside hydrolase with or comprising a substitution at one or more positions corresponding to one or more of residues F64, A226, and/or E246 in Thermobifida fusca Cel9A enzyme. In certain embodiments the glycoside hydrolase is a variant of a family 9 glycoside hydrolase. In certain embodiments the glycoside hydrolase is a variant of a theme B family 9 glycoside hydrolase.
- Inventors:
- Issue Date:
- Research Org.:
- Novozymes, A/S, Bagsvaerd (Denmark)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1193384
- Patent Number(s):
- 9080162
- Application Number:
- 14/111,321
- Assignee:
- Novozymes, A/S (Bagsvaerd, DK)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- DOE Contract Number:
- SC0002302
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Apr 11
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS
Citation Formats
Blazej, Robert, Toriello, Nicholas, Emrich, Charles, Cohen, Richard N., and Koppel, Nitzan. Cellulase variants. United States: N. p., 2015.
Web.
Blazej, Robert, Toriello, Nicholas, Emrich, Charles, Cohen, Richard N., & Koppel, Nitzan. Cellulase variants. United States.
Blazej, Robert, Toriello, Nicholas, Emrich, Charles, Cohen, Richard N., and Koppel, Nitzan. Tue .
"Cellulase variants". United States. https://www.osti.gov/servlets/purl/1193384.
@article{osti_1193384,
title = {Cellulase variants},
author = {Blazej, Robert and Toriello, Nicholas and Emrich, Charles and Cohen, Richard N. and Koppel, Nitzan},
abstractNote = {This invention provides novel variant cellulolytic enzymes having improved activity and/or stability. In certain embodiments the variant cellulotyic enzymes comprise a glycoside hydrolase with or comprising a substitution at one or more positions corresponding to one or more of residues F64, A226, and/or E246 in Thermobifida fusca Cel9A enzyme. In certain embodiments the glycoside hydrolase is a variant of a family 9 glycoside hydrolase. In certain embodiments the glycoside hydrolase is a variant of a theme B family 9 glycoside hydrolase.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 14 00:00:00 EDT 2015},
month = {Tue Jul 14 00:00:00 EDT 2015}
}
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Works referenced in this record:
Increased Crystalline Cellulose Activity via Combinations of Amino Acid Changes in the Family 9 Catalytic Domain and Family 3c Cellulose Binding Module of Thermobifida fusca Cel9A
journal, February 2010
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Integration of Computer Modeling and Initial Studies of Site-Directed Mutagenesis to Improve Cellulase Activity on Cel9A from <I>Thermobifida fusca </I>
journal, January 2004
- Escovar-Kousen, José M.; Wilson, David; Irwin, Diana
- Applied Biochemistry and Biotechnology, Vol. 113, Issue 1-3
Kinetic Studies of Thermobifida fusca Cel9A Active Site Mutant Enzymes
journal, August 2004
- Zhou, Weilin; Irwin, Diana C.; Escovar-Kousen, Jose
- Biochemistry, Vol. 43, Issue 30, p. 9655-9663
Processivity, Substrate Binding, and Mechanism of Cellulose Hydrolysis by Thermobifida fusca Cel9A
journal, March 2007
- Li, Y.; Irwin, D. C.; Wilson, D. B.
- Applied and Environmental Microbiology, Vol. 73, Issue 10