Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis
Abstract
A computational method and computer-based system of modeling DNA synthesis for the design and interpretation of PCR amplification, parallel DNA synthesis, and microarray chip analysis. The method and system include modules that address the bioinformatics, kinetics, and thermodynamics of DNA amplification and synthesis. Specifically, the steps of DNA selection, as well as the kinetics and thermodynamics of DNA hybridization and extensions, are addressed, which enable the optimization of the processing and the prediction of the products as a function of DNA sequence, mixing protocol, time, temperature and concentration of species.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Livermore National Security, LLC, Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1176277
- Patent Number(s):
- 7711491
- Application Number:
- 10/840,779
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G16 - INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS G16B - BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 97 MATHEMATICS AND COMPUTING; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Vandersall, Jennifer A., Gardner, Shea N., and Clague, David S. Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis. United States: N. p., 2010.
Web.
Vandersall, Jennifer A., Gardner, Shea N., & Clague, David S. Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis. United States.
Vandersall, Jennifer A., Gardner, Shea N., and Clague, David S. Tue .
"Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis". United States. https://www.osti.gov/servlets/purl/1176277.
@article{osti_1176277,
title = {Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis},
author = {Vandersall, Jennifer A. and Gardner, Shea N. and Clague, David S.},
abstractNote = {A computational method and computer-based system of modeling DNA synthesis for the design and interpretation of PCR amplification, parallel DNA synthesis, and microarray chip analysis. The method and system include modules that address the bioinformatics, kinetics, and thermodynamics of DNA amplification and synthesis. Specifically, the steps of DNA selection, as well as the kinetics and thermodynamics of DNA hybridization and extensions, are addressed, which enable the optimization of the processing and the prediction of the products as a function of DNA sequence, mixing protocol, time, temperature and concentration of species.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 04 00:00:00 EDT 2010},
month = {Tue May 04 00:00:00 EDT 2010}
}
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