Method of identifying hairpin DNA probes by partial fold analysis
Abstract
Methods of identifying molecular beacons in which a secondary structure prediction algorithm is employed to identify oligonucleotide sequences within a target gene having the requisite hairpin structure. Isolated oligonucleotides, molecular beacons prepared from those oligonucleotides, and their use are also disclosed.
- Inventors:
- Issue Date:
- Research Org.:
- University Of Rochester, Rochester, NY (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1176186
- Patent Number(s):
- 7442510
- Application Number:
- 11/553,904
- Assignee:
- University Of Rochester (Rochester, NY)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-02ER63410
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2006 Oct 27
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES
Citation Formats
Miller, Benjamin L., and Strohsahl, Christopher M. Method of identifying hairpin DNA probes by partial fold analysis. United States: N. p., 2008.
Web.
Miller, Benjamin L., & Strohsahl, Christopher M. Method of identifying hairpin DNA probes by partial fold analysis. United States.
Miller, Benjamin L., and Strohsahl, Christopher M. Tue .
"Method of identifying hairpin DNA probes by partial fold analysis". United States. https://www.osti.gov/servlets/purl/1176186.
@article{osti_1176186,
title = {Method of identifying hairpin DNA probes by partial fold analysis},
author = {Miller, Benjamin L. and Strohsahl, Christopher M.},
abstractNote = {Methods of identifying molecular beacons in which a secondary structure prediction algorithm is employed to identify oligonucleotide sequences within a target gene having the requisite hairpin structure. Isolated oligonucleotides, molecular beacons prepared from those oligonucleotides, and their use are also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 28 00:00:00 EDT 2008},
month = {Tue Oct 28 00:00:00 EDT 2008}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Effect of Secondary Structure on the Thermodynamics and Kinetics of PNA Hybridization to DNA Hairpins
journal, November 2001
- Kushon, Stuart A.; Jordan, Jason P.; Seifert, Jennifer L.
- Journal of the American Chemical Society, Vol. 123, Issue 44
Hybridization-Based Unquenching of DNA Hairpins on Au Surfaces: Prototypical “Molecular Beacon” Biosensors
journal, April 2003
- Du, Hui; Disney, Matthew D.; Miller, Benjamin L.
- Journal of the American Chemical Society, Vol. 125, Issue 14
Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon
journal, January 2000
- Park, Steven; Wong, May; Marras, Salvatore A. E.
- Journal of Clinical Microbiology, Vol. 38, Issue 8
Stem-loop oligonucleotides: a robust tool for molecular biology and biotechnology
journal, June 2002
- Broude, Natalia E.
- Trends in Biotechnology, Vol. 20, Issue 6
Hybridization of single-stranded DNA targets to immobilized complementary DNA probes: comparison of hairpin versus linear capture probes
journal, February 2001
- Riccelli, P. V.
- Nucleic Acids Research, Vol. 29, Issue 4
Thermodynamic basis of the enhanced specificity of structured DNA probes
journal, May 1999
- Bonnet, G.; Tyagi, S.; Libchaber, A.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 11
Single-mismatch detection using gold-quenched fluorescent oligonucleotides
journal, April 2001
- Dubertret, Benoit; Calame, Michel; Libchaber, Albert J.
- Nature Biotechnology, Vol. 19, Issue 4