Methods for identifying an essential gene in a prokaryotic microorganism
Abstract
Methods are provided for the rapid identification of essential or conditionally essential DNA segments in any species of haploid cell (one copy chromosome per cell) that is capable of being transformed by artificial means and is capable of undergoing DNA recombination. This system offers an enhanced means of identifying essential function genes in diploid pathogens, such as gram-negative and gram-positive bacteria.
- Inventors:
- Issue Date:
- Research Org.:
- California Institute of Technology (CalTech), Pasadena, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175636
- Patent Number(s):
- 6991900
- Application Number:
- 09/896,509
- Assignee:
- California Institute of Technology (Pasadena, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG03-99ER62855
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Shizuya, Hiroaki. Methods for identifying an essential gene in a prokaryotic microorganism. United States: N. p., 2006.
Web.
Shizuya, Hiroaki. Methods for identifying an essential gene in a prokaryotic microorganism. United States.
Shizuya, Hiroaki. Tue .
"Methods for identifying an essential gene in a prokaryotic microorganism". United States. https://www.osti.gov/servlets/purl/1175636.
@article{osti_1175636,
title = {Methods for identifying an essential gene in a prokaryotic microorganism},
author = {Shizuya, Hiroaki},
abstractNote = {Methods are provided for the rapid identification of essential or conditionally essential DNA segments in any species of haploid cell (one copy chromosome per cell) that is capable of being transformed by artificial means and is capable of undergoing DNA recombination. This system offers an enhanced means of identifying essential function genes in diploid pathogens, such as gram-negative and gram-positive bacteria.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 31 00:00:00 EST 2006},
month = {Tue Jan 31 00:00:00 EST 2006}
}
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Works referenced in this record:
Construction and Characterization of a Human Bacterial Artificial Chromosome Library
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- Kim, Ung-Jin; Birren, Bruce W.; Slepak, Tatiana
- Genomics, Vol. 34, Issue 2
The development and applications of the bacterial artificial chromosome cloning system.
journal, January 2001
- Shizuya, Hiroaki; Kouros-Mehr, Hosein
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