Protein crystallography prescreen kit
Abstract
A kit for prescreening protein concentration for crystallization includes a multiplicity of vials, a multiplicity of pre-selected reagents, and a multiplicity of sample plates. The reagents and a corresponding multiplicity of samples of the protein in solutions of varying concentrations are placed on sample plates. The sample plates containing the reagents and samples are incubated. After incubation the sample plates are examined to determine which of the sample concentrations are too low and which the sample concentrations are too high. The sample concentrations that are optimal for protein crystallization are selected and used.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175418
- Patent Number(s):
- 6916455
- Assignee:
- University Of California, The Regents Of
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
C - CHEMISTRY C30 - CRYSTAL GROWTH C30B - SINGLE-CRYSTAL-GROWTH
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Segelke, Brent W., Krupka, Heike I., and Rupp, Bernhard. Protein crystallography prescreen kit. United States: N. p., 2005.
Web.
Segelke, Brent W., Krupka, Heike I., & Rupp, Bernhard. Protein crystallography prescreen kit. United States.
Segelke, Brent W., Krupka, Heike I., and Rupp, Bernhard. Tue .
"Protein crystallography prescreen kit". United States. https://www.osti.gov/servlets/purl/1175418.
@article{osti_1175418,
title = {Protein crystallography prescreen kit},
author = {Segelke, Brent W. and Krupka, Heike I. and Rupp, Bernhard},
abstractNote = {A kit for prescreening protein concentration for crystallization includes a multiplicity of vials, a multiplicity of pre-selected reagents, and a multiplicity of sample plates. The reagents and a corresponding multiplicity of samples of the protein in solutions of varying concentrations are placed on sample plates. The sample plates containing the reagents and samples are incubated. After incubation the sample plates are examined to determine which of the sample concentrations are too low and which the sample concentrations are too high. The sample concentrations that are optimal for protein crystallization are selected and used.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 12 00:00:00 EDT 2005},
month = {Tue Jul 12 00:00:00 EDT 2005}
}
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