Stepped electrophoresis for movement and concentration of DNA
Abstract
A fluidic channel patterned with a series of thin-film electrodes makes it possible to move and concentrate DNA in a fluid passing through the fluidic channel. The DNA has an inherent negative charge and by applying a voltage between adjacent electrodes the DNA is caused to move. By using a series of electrodes, when one electrode voltage or charge is made negative with respect to adjacent electrodes, the DNA is repelled away from this electrode and attached to a positive charged electrode of the series. By sequentially making the next electrode of the series negative, the DNA can be moved to and concentrated over the remaining positive electrodes.
- Inventors:
- Issue Date:
- Research Org.:
- The Regents of the Univ. of California, Oakland, CA (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175272
- Patent Number(s):
- 6866759
- Application Number:
- 09/738,462
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Miles, Robin R., Wang, Amy Wei-Yun, and Mariella, Jr., Raymond P. Stepped electrophoresis for movement and concentration of DNA. United States: N. p., 2005.
Web.
Miles, Robin R., Wang, Amy Wei-Yun, & Mariella, Jr., Raymond P. Stepped electrophoresis for movement and concentration of DNA. United States.
Miles, Robin R., Wang, Amy Wei-Yun, and Mariella, Jr., Raymond P. Tue .
"Stepped electrophoresis for movement and concentration of DNA". United States. https://www.osti.gov/servlets/purl/1175272.
@article{osti_1175272,
title = {Stepped electrophoresis for movement and concentration of DNA},
author = {Miles, Robin R. and Wang, Amy Wei-Yun and Mariella, Jr., Raymond P.},
abstractNote = {A fluidic channel patterned with a series of thin-film electrodes makes it possible to move and concentrate DNA in a fluid passing through the fluidic channel. The DNA has an inherent negative charge and by applying a voltage between adjacent electrodes the DNA is caused to move. By using a series of electrodes, when one electrode voltage or charge is made negative with respect to adjacent electrodes, the DNA is repelled away from this electrode and attached to a positive charged electrode of the series. By sequentially making the next electrode of the series negative, the DNA can be moved to and concentrated over the remaining positive electrodes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 15 00:00:00 EST 2005},
month = {Tue Mar 15 00:00:00 EST 2005}
}
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