Title: Pyrococcus furiosus strains and methods of using same

Abstract

Provided herein are methods for transforming a Pyrococcus furiosus with a polynucleotide. In one embodiment, the method includes contacting a P. furiosus with a polynucleotide under conditions suitable for uptake of the polynucleotide by the P. furiosus, and identifying transformants at a frequency of, for instance, at least 10.sup.3 transformants per microgram DNA. Also provided are isolated Pyrococcus furiosus having the characteristics of Pyrococcus furiosus COM1, and plasmids that include an origin of replication that functions in a Pyrococcus furiosus. The plasmid is stable in a recipient P. furiosus without selection for more than 100 generations and is structurally unchanged after replication in P. furiosus for more than 100 generations.

Inventors:

Lipscomb, Gina L; Farkas, Joel Andrew; Adams, Michael W. W.; Westpheling, Janet

Issue Date:

Tue Jan 06 00:00:00 EST 2015

Research Org.:

Univ. of Georgia, Athens, GA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1167026

Patent Number(s):

8927254

Application Number:

13/248,377

Assignee:

University of Georgia Research Foundation, Inc. (Athens, GA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

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DOE Contract Number:  

FG05-95ER20175

Resource Type:

Patent

Resource Relation:

Patent File Date: 2011 Sep 29

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES