Subcellular analysis by laser ablation electrospray ionization mass spectrometry

Vertes, Akos; Stolee, Jessica A; Shrestha, Bindesh

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Title: Subcellular analysis by laser ablation electrospray ionization mass spectrometry

Abstract

In various embodiments, a method of laser ablation electrospray ionization mass spectrometry (LAESI-MS) may generally comprise micro-dissecting a cell comprising at least one of a cell wall and a cell membrane to expose at least one subcellular component therein, ablating the at least one subcellular component by an infrared laser pulse to form an ablation plume, intercepting the ablation plume by an electrospray plume to form ions, and detecting the ions by mass spectrometry.

Inventors:: Vertes, Akos; Stolee, Jessica A; Shrestha, Bindesh

Issue Date:: Tue Dec 02 00:00:00 EST 2014

Research Org.:: George Washington Univ., Washington, DC (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1164685

Patent Number(s):: 8901487

Application Number:: 13/045,277

Assignee:: George Washington University (Washington, DC)

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2001/045 - {Laser ablation

H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
H01J49/0004 - {Imaging particle spectrometry}
H01J49/0463 - {Desorption by laser or particle beam, followed by ionisation as a separate step
H01J49/165 - {Electrospray ionisation}
H01J49/26 - Mass spectrometers or separator tubes

H - ELECTRICITY H05 - ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR H05H - PLASMA TECHNIQUE
H05H3/02 - Molecular or atomic beam generation {

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DOE Contract Number:: FG02-01ER15129

Resource Type:: Patent

Resource Relation:: Patent File Date: 2011 Mar 10

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 47 OTHER INSTRUMENTATION

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                    Vertes, Akos, Stolee, Jessica A, and Shrestha, Bindesh. Subcellular analysis by laser ablation electrospray ionization mass spectrometry.  United States: N. p., 2014. 
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                    Vertes, Akos, Stolee, Jessica A, & Shrestha, Bindesh. Subcellular analysis by laser ablation electrospray ionization mass spectrometry.  United States.

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                    Vertes, Akos, Stolee, Jessica A, and Shrestha, Bindesh. Tue .  
        "Subcellular analysis by laser ablation electrospray ionization mass spectrometry".  United States.  https://www.osti.gov/servlets/purl/1164685.

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@article{osti_1164685,

  title        = {Subcellular analysis by laser ablation electrospray ionization mass spectrometry},

  author       = {Vertes, Akos and Stolee, Jessica A and Shrestha, Bindesh},

  abstractNote = {In various embodiments, a method of laser ablation electrospray ionization mass spectrometry (LAESI-MS) may generally comprise micro-dissecting a cell comprising at least one of a cell wall and a cell membrane to expose at least one subcellular component therein, ablating the at least one subcellular component by an infrared laser pulse to form an ablation plume, intercepting the ablation plume by an electrospray plume to form ions, and detecting the ions by mass spectrometry.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Dec 02 00:00:00 EST 2014},

  month        = {Tue Dec 02 00:00:00 EST 2014}

}

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Infrared Laser Ablation Atmospheric Pressure Photoionization Mass Spectrometry
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Elevated subcortical choline metabolites in cognitively and clinically asymptomatic HIV patients
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Neurology, Vol. 52, Issue 5
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Laser Capture Microdissection
journal, November 1996

Emmert-Buck, Michael R.; Bonner, Robert F.; Smith, Paul D.
Science, Vol. 274, Issue 5289
https://doi.org/10.1126/science.274.5289.998

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Title: Subcellular analysis by laser ablation electrospray ionization mass spectrometry

Abstract

Citation Formats

Frequency standard using an atomic fountain of optically trapped atoms patent, August 1994

Concentration of biological samples on a microliter scale and analysis by capillary electrophoresis patent, June 1998

Atmospheric pressure matrix assisted laser desorption patent, October 1999

Fast controllable laser lysis of cells for analysis patent, December 2000

Ion mobility spectrometer, spectrometer analyte detection and identification verification system, and method patent, December 2002

Methods and apparatus for cell analysis patent, March 2003

Miniaturized cell array methods and apparatus for cell-based screening patent, April 2003

Automated sample processing for identification of microorganisms and proteins patent, May 2003

Mass spectrometric methods for biomolecular screening patent, December 2003

Method and apparatus for feedback controlled electrospray patent, June 2004

Atmospheric pressure ion source patent, September 2005

Solid phase sequencing of double-stranded nucleic acids patent, January 2006

Method for increasing ionization efficiency in mass spectroscopy patent, August 2006

Apparatus and method for sensor control and feedback patent, August 2006

Method for atmospheric pressure analyte ionization patent, September 2006

Laser desorption and ionization mass spectrometer with quantitative reproducibility patent, October 2006

MALDI-IM-ortho-TOF mass spectrometry with simultaneous positive and negative mode detection patent, January 2007

Combined chemical/biological agent detection system and method utilizing mass spectrometry patent, September 2007

Method and system for desorption electrospray ionization patent, February 2008

Mass spectrometric based method for sample identification patent, March 2008

Gold implantation/deposition of biological samples for laser desorption two and three dimensional depth profiling of biological tissues patent, December 2009

Pattern recognition of whole cell mass spectra patent, March 2010

Mass spectrometric imaging method under ambient conditions using electrospray-assisted laser desorption ionization mass spectrometry patent, March 2010

Electrospray-assisted laser desorption ionization device, mass spectrometer, and method for mass spectrometry patent, April 2010

Methods for direct biomolecule identification by matrix-assisted laser desorption ionization (MALDI) mass spectrometry patent, May 2010

Protein microscope patent, June 2010

Compositions and methods for the treatment and prevention of cancer, angiogenesis, and inflammation patent, March 2011

Natural marine source phospholipids comprising polyunsaturated fatty acids and their applications patent, October 2011

Method and apparatus for feedback controlled electrospray patent-application, December 2002

Method for searching for gene encoding nuclear transport protein patent-application, September 2003

Method and device for manipulating microscopic quantities of material patent-application, March 2004

Method and compositions for identifying anti-HIV therapeutic compounds patent-application, June 2004

Methods and apparatuses for analyzing biological samples by mass spectrometry patent-application, February 2005

Gold implantation/deposition of biological samples for laser desorption three dimensional depth profiling of tissues patent-application, February 2005

Method and device for biochemical detection and analysis of subcellular compartments from a single cell patent-application, March 2005

MALDI-IM-ortho-TOF mass spectrometry with simultaneous positive and negative mode detection patent-application, October 2005

Method and system for desorption electrospray ionization patent-application, October 2005

Combined chemical/biological agent detection system and method utilizing mass spectrometry patent-application, November 2005

Laser desorption and ionization mass spectrometer with quantitative reproducibility patent-application, December 2005

Gold implantation/deposition of biological samples for laser desorption two and three dimensional depth profiling of biological tissues patent-application, June 2006

Peptide sequencing from peptide fragmentation mass spectra patent-application, August 2006

Computational method and system for mass spectral analysis patent-application, September 2006

Methods for direct biomolecule identification by matrix-assisted laser desorption ionization (MALDI) mass spectrometry patent-application, May 2007

Electrospray-Assisted Laser Desorption Ionization Device, Mass Spectrometer, and Method for Mass Spectrometry patent-application, August 2007

Reagents and Methods for Using Human Embryonic Stem Cells to Evaluate Toxicity of Pharmaceutical Compounds and Other Chemicals patent-application, October 2007

Mass spectrometric imaging method under ambient conditions using electrospray-assisted laser desorption ionization mass spectrometry patent-application, January 2008

Method of Analyzing Biosample by Laser Ablation and Apparatus Therefor patent-application, January 2008

Laser Desorption Device, Mass Spectrometer Assembly, and Method for Ambient Liquid Mass Spectrometry patent-application, May 2008

Hypolipidemic and/or hypocholesteremic compounds obtainable from the goldenseal plant patent-application, May 2008

Mass Spectrometry With Laser Ablation patent-application, June 2008

Protein Microscope patent-application, June 2008

Rare Cell Analysis Using Sample Splitting and DNA Tags patent-application, September 2008

Laser desorption - electrospray ion (ESI) source for mass spectrometers patent-application, November 2008

Electrospray-Assisted Laser-Induced Acoustic Desorption Ionization Mass Spectrometer and a Method for Mass Spectrometry patent-application, December 2008

Lamp patent-application, January 2009

Imaging Mass Spectrometry Principle and Its Application in a Device patent-application, October 2009

Laser Ablation Electrospray Ionization (LAESI) for Atmospheric Pressure, In Vivo, and Imaging Mass Spectrometry patent-application, November 2009

Laser ablation flowing atmospheric-pressure afterglow for ambient mass spectrometry patent-application, November 2009

Laser Desorption Ionization and Peptide Sequencing on Laser Induced Silicon Microcolumn Arrays patent-application, December 2009

Three-Dimensional Molecular Imaging By Infrared Laser Ablation Electrospray Ionization Mass Spectrometry patent-application, January 2010

Gold Implantation/Deposition of Biological Samples for Laser Desorption Two and Three Dimensional Depth Profiling of Biological Tissues patent-application, April 2010

Ionization Device patent-application, April 2010

Method and device for separation and depletion of certain proteins and particles using electrophoresis patent-application, October 2010

Methods for Detecting Metabolic States by Laser Ablation Electrospray Ionization Mass Spectrometry patent-application, November 2010

Three-Dimensional Molecular Imaging By Infrared Laser Ablation Electrospray Ionization Mass Spectrometry patent-application, November 2011

Three-Dimensional Molecular Imaging By Infrared Laser Ablation Electrospray Ionization Mass Spectrometry patent-application, November 2012

Free-electron-laser-based biophysical and biomedical instrumentation journal, July 2003

FT-IR imaging of native and tissue-engineered bone and cartilage journal, May 2007

Matrix-assisted laser desorption and ionization in the OH and CO absorption bands of aliphatic and aromatic matrices: dependence on laser wavelength and temporal beam profile journal, December 1997

Ablation and analysis of small cell populations and single cells by consecutive laser pulses journal, June 2010

Nanoscale Atmospheric Pressure Laser Ablation-Mass Spectrometry journal, April 2001

Laser Desorption-Atmospheric Pressure Chemical Ionization Mass Spectrometry for the Analysis of Peptides from Aqueous Solutions journal, November 2002

New dimension in nano-imaging: breaking through the diffraction limit with scanning near-field optical microscopy journal, November 2004

Direct Protein Detection from Biological Media through Electrospray-Assisted Laser Desorption Ionization/Mass Spectrometry journal, May 2006

Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization journal, October 2004

Versatile New Ion Source for the Analysis of Materials in Open Air under Ambient Conditions journal, April 2005

Laser Ablation Electrospray Ionization for Atmospheric Pressure, in Vivo, and Imaging Mass Spectrometry journal, November 2007

Frequency standard using an atomic fountain of optically trapped atoms
patent, August 1994

Concentration of biological samples on a microliter scale and analysis by capillary electrophoresis
patent, June 1998

Atmospheric pressure matrix assisted laser desorption
patent, October 1999

Fast controllable laser lysis of cells for analysis
patent, December 2000

Ion mobility spectrometer, spectrometer analyte detection and identification verification system, and method
patent, December 2002

Methods and apparatus for cell analysis
patent, March 2003

Miniaturized cell array methods and apparatus for cell-based screening
patent, April 2003

Automated sample processing for identification of microorganisms and proteins
patent, May 2003

Mass spectrometric methods for biomolecular screening
patent, December 2003

Method and apparatus for feedback controlled electrospray
patent, June 2004

Atmospheric pressure ion source
patent, September 2005

Solid phase sequencing of double-stranded nucleic acids
patent, January 2006

Method for increasing ionization efficiency in mass spectroscopy
patent, August 2006

Apparatus and method for sensor control and feedback
patent, August 2006

Method for atmospheric pressure analyte ionization
patent, September 2006

Laser desorption and ionization mass spectrometer with quantitative reproducibility
patent, October 2006

MALDI-IM-ortho-TOF mass spectrometry with simultaneous positive and negative mode detection
patent, January 2007

Combined chemical/biological agent detection system and method utilizing mass spectrometry
patent, September 2007

Method and system for desorption electrospray ionization
patent, February 2008

Mass spectrometric based method for sample identification
patent, March 2008

Gold implantation/deposition of biological samples for laser desorption two and three dimensional depth profiling of biological tissues
patent, December 2009

Pattern recognition of whole cell mass spectra
patent, March 2010

Mass spectrometric imaging method under ambient conditions using electrospray-assisted laser desorption ionization mass spectrometry
patent, March 2010

Electrospray-assisted laser desorption ionization device, mass spectrometer, and method for mass spectrometry
patent, April 2010

Methods for direct biomolecule identification by matrix-assisted laser desorption ionization (MALDI) mass spectrometry
patent, May 2010

Protein microscope
patent, June 2010

Compositions and methods for the treatment and prevention of cancer, angiogenesis, and inflammation
patent, March 2011

Natural marine source phospholipids comprising polyunsaturated fatty acids and their applications
patent, October 2011

Method and apparatus for feedback controlled electrospray
patent-application, December 2002

Method for searching for gene encoding nuclear transport protein
patent-application, September 2003

Method and device for manipulating microscopic quantities of material
patent-application, March 2004

Method and compositions for identifying anti-HIV therapeutic compounds
patent-application, June 2004

Methods and apparatuses for analyzing biological samples by mass spectrometry
patent-application, February 2005

Gold implantation/deposition of biological samples for laser desorption three dimensional depth profiling of tissues
patent-application, February 2005

Method and device for biochemical detection and analysis of subcellular compartments from a single cell
patent-application, March 2005

MALDI-IM-ortho-TOF mass spectrometry with simultaneous positive and negative mode detection
patent-application, October 2005

Method and system for desorption electrospray ionization
patent-application, October 2005

Combined chemical/biological agent detection system and method utilizing mass spectrometry
patent-application, November 2005

Laser desorption and ionization mass spectrometer with quantitative reproducibility
patent-application, December 2005

Gold implantation/deposition of biological samples for laser desorption two and three dimensional depth profiling of biological tissues
patent-application, June 2006

Peptide sequencing from peptide fragmentation mass spectra
patent-application, August 2006

Computational method and system for mass spectral analysis
patent-application, September 2006

Methods for direct biomolecule identification by matrix-assisted laser desorption ionization (MALDI) mass spectrometry
patent-application, May 2007

Electrospray-Assisted Laser Desorption Ionization Device, Mass Spectrometer, and Method for Mass Spectrometry
patent-application, August 2007

Reagents and Methods for Using Human Embryonic Stem Cells to Evaluate Toxicity of Pharmaceutical Compounds and Other Chemicals
patent-application, October 2007

Mass spectrometric imaging method under ambient conditions using electrospray-assisted laser desorption ionization mass spectrometry
patent-application, January 2008

Method of Analyzing Biosample by Laser Ablation and Apparatus Therefor
patent-application, January 2008

Laser Desorption Device, Mass Spectrometer Assembly, and Method for Ambient Liquid Mass Spectrometry
patent-application, May 2008

Hypolipidemic and/or hypocholesteremic compounds obtainable from the goldenseal plant
patent-application, May 2008

Mass Spectrometry With Laser Ablation
patent-application, June 2008

Protein Microscope
patent-application, June 2008

Rare Cell Analysis Using Sample Splitting and DNA Tags
patent-application, September 2008

Laser desorption - electrospray ion (ESI) source for mass spectrometers
patent-application, November 2008

Electrospray-Assisted Laser-Induced Acoustic Desorption Ionization Mass Spectrometer and a Method for Mass Spectrometry
patent-application, December 2008

Lamp
patent-application, January 2009

Imaging Mass Spectrometry Principle and Its Application in a Device
patent-application, October 2009

Laser Ablation Electrospray Ionization (LAESI) for Atmospheric Pressure, In Vivo, and Imaging Mass Spectrometry
patent-application, November 2009

Laser ablation flowing atmospheric-pressure afterglow for ambient mass spectrometry
patent-application, November 2009

Laser Desorption Ionization and Peptide Sequencing on Laser Induced Silicon Microcolumn Arrays
patent-application, December 2009

Three-Dimensional Molecular Imaging By Infrared Laser Ablation Electrospray Ionization Mass Spectrometry
patent-application, January 2010

Gold Implantation/Deposition of Biological Samples for Laser Desorption Two and Three Dimensional Depth Profiling of Biological Tissues
patent-application, April 2010

Ionization Device
patent-application, April 2010

Method and device for separation and depletion of certain proteins and particles using electrophoresis
patent-application, October 2010

Methods for Detecting Metabolic States by Laser Ablation Electrospray Ionization Mass Spectrometry
patent-application, November 2010

Three-Dimensional Molecular Imaging By Infrared Laser Ablation Electrospray Ionization Mass Spectrometry
patent-application, November 2011

Three-Dimensional Molecular Imaging By Infrared Laser Ablation Electrospray Ionization Mass Spectrometry
patent-application, November 2012

Free-electron-laser-based biophysical and biomedical instrumentation
journal, July 2003

FT-IR imaging of native and tissue-engineered bone and cartilage
journal, May 2007

Matrix-assisted laser desorption and ionization in the OH and CO absorption bands of aliphatic and aromatic matrices: dependence on laser wavelength and temporal beam profile
journal, December 1997

Ablation and analysis of small cell populations and single cells by consecutive laser pulses
journal, June 2010

Nanoscale Atmospheric Pressure Laser Ablation-Mass Spectrometry
journal, April 2001

Laser Desorption-Atmospheric Pressure Chemical Ionization Mass Spectrometry for the Analysis of Peptides from Aqueous Solutions
journal, November 2002

New dimension in nano-imaging: breaking through the diffraction limit with scanning near-field optical microscopy
journal, November 2004

Direct Protein Detection from Biological Media through Electrospray-Assisted Laser Desorption Ionization/Mass Spectrometry
journal, May 2006

Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization
journal, October 2004

Versatile New Ion Source for the Analysis of Materials in Open Air under Ambient Conditions
journal, April 2005

Laser Ablation Electrospray Ionization for Atmospheric Pressure, in Vivo, and Imaging Mass Spectrometry
journal, November 2007

Recent Advances in Optical Tweezers
journal, June 2008

Simultaneous Imaging of Small Metabolites and Lipids in Rat Brain Tissues at Atmospheric Pressure by Laser Ablation Electrospray Ionization Mass Spectrometry
journal, February 2010

In Situ Metabolic Profiling of Single Cells by Laser Ablation Electrospray Ionization Mass Spectrometry
journal, October 2009