Methods for targetted mutagenesis in gram-positive bacteria
Abstract
The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1132802
- Patent Number(s):
- 8735160
- Application Number:
- 12/233,806
- Assignee:
- UT-Battelle, LLC (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC05-000R22725
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2008 Sep 19
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Yang, Yunfeng. Methods for targetted mutagenesis in gram-positive bacteria. United States: N. p., 2014.
Web.
Yang, Yunfeng. Methods for targetted mutagenesis in gram-positive bacteria. United States.
Yang, Yunfeng. Tue .
"Methods for targetted mutagenesis in gram-positive bacteria". United States. https://www.osti.gov/servlets/purl/1132802.
@article{osti_1132802,
title = {Methods for targetted mutagenesis in gram-positive bacteria},
author = {Yang, Yunfeng},
abstractNote = {The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 27 00:00:00 EDT 2014},
month = {Tue May 27 00:00:00 EDT 2014}
}
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Works referenced in this record:
Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824
journal, August 1996
- Green, E. M.; Boynton, Z. L.; Harris, L. M.
- Microbiology, Vol. 142, Issue 8
Northern, Morphological, and Fermentation Analysis of spo0A Inactivation and Overexpression in Clostridium acetobutylicum ATCC 824
journal, July 2002
- Harris, L. M.; Welker, N. E.; Papoutsakis, E. T.
- Journal of Bacteriology, Vol. 184, Issue 13
Gene transfer to Clostridium cellulolyticum ATCC 35319
journal, December 2000
- Jennert, Katrin C. B.; Young, Danielle I.; Young, Michael
- Microbiology, Vol. 146, Issue 12
Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K
journal, December 1978
- Kolter, Roberto; Inuzuka, Manabu; Helinski, Donald R.
- Cell, Vol. 15, Issue 4, p. 1199-1208
Studies on cellulase production by Clostridium thermocellum
journal, January 1980
- Garcia-Martinez, D. V.; Shinmyo, A.; Madia, A.
- European Journal of Applied Microbiology and Biotechnology, Vol. 9, Issue 3
Use of antisense RNA to modify the composition of cellulosomes produced by Clostridium cellulolyticum: asRNA downregulation of a C. cellulolyticum cellulase
journal, January 2004
- Perret, Stéphanie; Maamar, Hédia; Bélaich, Jean-Pierre
- Molecular Microbiology, Vol. 51, Issue 2
Electrotransformation studies in Clostridium cellulolyticum
journal, November 2001
- Tardif, C.; Maamar, H.; Balfin, M.
- Journal of Industrial Microbiology and Biotechnology, Vol. 27, Issue 5
Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
- Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
- Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577