Micromachined patch-clamp apparatus
Abstract
A micromachined patch-clamp apparatus is disclosed for holding one or more cells and providing electrical, chemical, or mechanical stimulation to the cells during analysis with the patch-clamp technique for studying ion channels in cell membranes. The apparatus formed on a silicon substrate utilizes a lower chamber formed from silicon nitride using surface micromachining and an upper chamber formed from a molded polymer material. An opening in a common wall between the chambers is used to trap and hold a cell for analysis using the patch-clamp technique with sensing electrodes on each side of the cell. Some embodiments of the present invention utilize one or more electrostatic actuators formed on the substrate to provide mechanical stimulation to the cell being analyzed, or to provide information about mechanical movement of the cell in response to electrical or chemical stimulation.
- Inventors:
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1082484
- Patent Number(s):
- 8323955
- Application Number:
- 10/383,163
- Assignee:
- Sandia Corporation (Albuquerque, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION
Citation Formats
Okandan, Murat. Micromachined patch-clamp apparatus. United States: N. p., 2012.
Web.
Okandan, Murat. Micromachined patch-clamp apparatus. United States.
Okandan, Murat. Tue .
"Micromachined patch-clamp apparatus". United States. https://www.osti.gov/servlets/purl/1082484.
@article{osti_1082484,
title = {Micromachined patch-clamp apparatus},
author = {Okandan, Murat},
abstractNote = {A micromachined patch-clamp apparatus is disclosed for holding one or more cells and providing electrical, chemical, or mechanical stimulation to the cells during analysis with the patch-clamp technique for studying ion channels in cell membranes. The apparatus formed on a silicon substrate utilizes a lower chamber formed from silicon nitride using surface micromachining and an upper chamber formed from a molded polymer material. An opening in a common wall between the chambers is used to trap and hold a cell for analysis using the patch-clamp technique with sensing electrodes on each side of the cell. Some embodiments of the present invention utilize one or more electrostatic actuators formed on the substrate to provide mechanical stimulation to the cell being analyzed, or to provide information about mechanical movement of the cell in response to electrical or chemical stimulation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 04 00:00:00 EST 2012},
month = {Tue Dec 04 00:00:00 EST 2012}
}
Works referenced in this record:
Micromolded PDMS planar electrode allows patch clamp electrical recordings from cells
journal, June 2002
- Klemic, Kathryn G.; Klemic, James F.; Reed, Mark A.
- Biosensors and Bioelectronics, Vol. 17, Issue 6-7
Microfabricated electroporation chip for single cell membrane permeabilization
journal, April 2001
- Huang, Yong; Rubinsky, Boris
- Sensors and Actuators A: Physical, Vol. 89, Issue 3