Multiplex detection of respiratory pathogens
Abstract
Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.
- Inventors:
-
- Brentwood, CA
- Livermore, CA
- Albany, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1058899
- Patent Number(s):
- 8232058
- Application Number:
- 12/161,291
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
McBride, Mary, Slezak, Thomas, and Birch, James M. Multiplex detection of respiratory pathogens. United States: N. p., 2012.
Web.
McBride, Mary, Slezak, Thomas, & Birch, James M. Multiplex detection of respiratory pathogens. United States.
McBride, Mary, Slezak, Thomas, and Birch, James M. Tue .
"Multiplex detection of respiratory pathogens". United States. https://www.osti.gov/servlets/purl/1058899.
@article{osti_1058899,
title = {Multiplex detection of respiratory pathogens},
author = {McBride, Mary and Slezak, Thomas and Birch, James M},
abstractNote = {Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 31 00:00:00 EDT 2012},
month = {Tue Jul 31 00:00:00 EDT 2012}
}
Works referenced in this record:
A Sensitive, Specific, and Cost-Effective Multiplex Reverse Transcriptase-PCR Assay for the Detection of Seven Common Respiratory Viruses in Respiratory Samples
journal, May 2004
- Syrmis, Melanie W.; Whiley, David M.; Thomas, Marion
- The Journal of Molecular Diagnostics, Vol. 6, Issue 2
Rapid Detection and Identification of Human Adenovirus Species by Adenoplex, a Multiplex PCR-Enzyme Hybridization Assay
journal, September 2004
- Pehler-Harrington, K.; Khanna, M.; Waters, C. R.
- Journal of Clinical Microbiology, Vol. 42, Issue 9
Multiplexed Reverse Transcriptase PCR Assay for Identification of Viral Respiratory Pathogens at the Point of Care
journal, September 2007
- Letant, S. E.; Ortiz, J. I.; Bentley Tammero, L. F.
- Journal of Clinical Microbiology, Vol. 45, Issue 11
Genetic analysis of human H2N2 and early H3N2 influenza viruses, 1957–1972: evidence for genetic divergence and multiple reassortment events
journal, October 2004
- Lindstrom, Stephen E.; Cox, Nancy J.; Klimov, Alexander
- Virology, Vol. 328, Issue 1
Influenza A viruses in feral Canadian ducks: extensive reassortment in nature
journal, August 2004
- Hatchette, Todd F.; Walker, David; Johnson, Christie
- Journal of General Virology, Vol. 85, Issue 8
A computer program for selection of oligonucleotide primers for polymerase chain reactions
journal, January 1990
- Lowe, Todd; Sharefkin, John; Yang, Shi Qi
- Nucleic Acids Research, Vol. 18, Issue 7
Large-Scale Sequence Analysis of Avian Influenza Isolates
journal, March 2006
- Obenauer, J. C.
- Science, Vol. 311, Issue 5767
A multiplexed PCR-coupled liquid bead array for the simultaneous detection of four biothreat agents
journal, April 2005
- Wilson, Wendy J.; Erler, Anne M.; Nasarabadi, Shanavaz L.
- Molecular and Cellular Probes, Vol. 19, Issue 2, p. 137-144
Comparison of Real-Time PCR Assays with Fluorescent-Antibody Assays for Diagnosis of Respiratory Virus Infections in Children
journal, July 2006
- Kuypers, J.; Wright, N.; Ferrenberg, J.
- Journal of Clinical Microbiology, Vol. 44, Issue 7
Evolutionary characterization of the six internal genes of H5N1 human influenza A virus
journal, May 2000
- Nerome, Reiko; Hiromoto, Yasuaki; Fukushima, Tatsunobu
- Journal of General Virology, Vol. 81, Issue 5
Research Profiles: Liquid array single-handedly detects bounty of BW agents
journal, May 2003
- Harris, Cheryl M.
- Analytical Chemistry, Vol. 75, Issue 9
Rapid and Sensitive Method Using Multiplex Real-Time PCR for Diagnosis of Infections by Influenza A and Influenza B Viruses, Respiratory Syncytial Virus, and Parainfluenza Viruses 1, 2, 3, and 4
journal, April 2004
- Templeton, K. E.; Scheltinga, S. A.; Beersma, M. F. C.
- Journal of Clinical Microbiology, Vol. 42, Issue 4
Characterizations of the human parainfluenza type 2 virus gene encoding the L protein and the intergenic sequences
journal, May 1991
- Kawano, M.; Okamoto, K.; Bando, H.
- Nucleic Acids Research, Vol. 19, Issue 10
Stable and Noncompetitive RNA Internal Control for Routine Clinical Diagnostic Reverse Transcription-PCR
journal, March 2004
- Dingle, K. E.; Crook, D.; Jeffery, K.
- Journal of Clinical Microbiology, Vol. 42, Issue 3
Design Strategies and Performance of Custom DNA Sequencing Primers
journal, September 1999
- Buck, G. A.; Fox, J. W.; Gunthorpe, M.
- BioTechniques, Vol. 27, Issue 3
Evolution of H5 subtype avian influenza A viruses in North America
journal, October 1997
- García, M.; Suarez, D. L.; Crawford, J. M.
- Virus Research, Vol. 51, Issue 2
Multiplexed Liquid Arrays for Simultaneous Detection of Simulants of Biological Warfare Agents
journal, April 2003
- McBride, Mary T.; Gammon, Stuart; Pitesky, Maurice
- Analytical Chemistry, Vol. 75, Issue 8
Antigenic and genetic diversity among swine influenza A H1N1 and H1N2 viruses in Europe
journal, April 2002
- Marozin, S.; Gregory, V.; Cameron, K.
- Journal of General Virology, Vol. 83, Issue 4
Clinical and Financial Benefits of Rapid Detection of Respiratory Viruses: an Outcomes Study
journal, January 2000
- Barenfanger, Joan; Drake, Cheryl; Leon, Nidia
- Journal of Clinical Microbiology, Vol. 38, Issue 8
Molecular changes associated with the transmission of avian influenza a H5N1 and H9N2 viruses to humans
journal, November 2001
- Shaw, M.; Cooper, L.; Xu, X.
- Journal of Medical Virology, Vol. 66, Issue 1
Direct Detection of Respiratory Syncytial Virus, Parainfluenza Virus, and Adenovirus in Clinical Respiratory Specimens by a Multiplex Reverse Transcription-PCR Assay
journal, January 1998
- Osiowy, Carla
- Journal of Clinical Microbiology, Vol. 36, Issue 11
Comparative Analysis of Evolutionary Mechanisms of the Hemagglutinin and Three Internal Protein Genes of Influenza B Virus: Multiple Cocirculating Lineages and Frequent Reassortment of the NP, M, and NS Genes
journal, May 1999
- Lindstrom, Stephen E.; Hiromoto, Yasuaki; Nishimura, Hidekazu
- Journal of Virology, Vol. 73, Issue 5
Transmission of Eurasian avian H2 influenza virus to shorebirds in North America
journal, December 1999
- Makarova, N. V.; Kaverin, N. V.; Krauss, S.
- Journal of General Virology, Vol. 80, Issue 12
Origin of the Pandemic 1957 H2 Influenza A Virus and the Persistence of Its Possible Progenitors in the Avian Reservoir
journal, June 1993
- Schäffr, Judith R.; Kawaoka, Yoshihiro; Bean, William J.
- Virology, Vol. 194, Issue 2
Comparison of the Directigen Flu A+B Test, the QuickVue Influenza Test, and Clinical Case Definition to Viral Culture and Reverse Transcription-PCR for Rapid Diagnosis of Influenza Virus Infection
journal, August 2003
- Ruest, A.; Michaud, S.; Deslandes, S.
- Journal of Clinical Microbiology, Vol. 41, Issue 8