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Title: Multiplex detection of respiratory pathogens

Abstract

Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

Inventors:
 [1];  [2];  [3]
  1. Brentwood, CA
  2. Livermore, CA
  3. Albany, CA
Issue Date:
Research Org.:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1058899
Patent Number(s):
8232058
Application Number:
12/161,291
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

McBride, Mary, Slezak, Thomas, and Birch, James M. Multiplex detection of respiratory pathogens. United States: N. p., 2012. Web.
McBride, Mary, Slezak, Thomas, & Birch, James M. Multiplex detection of respiratory pathogens. United States.
McBride, Mary, Slezak, Thomas, and Birch, James M. Tue . "Multiplex detection of respiratory pathogens". United States. https://www.osti.gov/servlets/purl/1058899.
@article{osti_1058899,
title = {Multiplex detection of respiratory pathogens},
author = {McBride, Mary and Slezak, Thomas and Birch, James M},
abstractNote = {Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 31 00:00:00 EDT 2012},
month = {Tue Jul 31 00:00:00 EDT 2012}
}

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