Site specific incorporation of keto amino acids into proteins
Abstract
Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.
- Inventors:
-
- La Jolla, CA
- San Diego, CA
- Issue Date:
- Research Org.:
- The Scripps Research Inst., La Jolla, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1034342
- Patent Number(s):
- 8071344
- Application Number:
- 11/978,182
- Assignee:
- The Scripps Research Institute (La Jolla, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG03-00ER45812; AC03-76SF00098
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Schultz, Peter G, and Wang, Lei. Site specific incorporation of keto amino acids into proteins. United States: N. p., 2011.
Web.
Schultz, Peter G, & Wang, Lei. Site specific incorporation of keto amino acids into proteins. United States.
Schultz, Peter G, and Wang, Lei. Tue .
"Site specific incorporation of keto amino acids into proteins". United States. https://www.osti.gov/servlets/purl/1034342.
@article{osti_1034342,
title = {Site specific incorporation of keto amino acids into proteins},
author = {Schultz, Peter G and Wang, Lei},
abstractNote = {Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 06 00:00:00 EST 2011},
month = {Tue Dec 06 00:00:00 EST 2011}
}
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Works referenced in this record:
Twenty-first aminoacyl-tRNA synthetase-suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria
journal, January 2001
- Kowal, A. K.; Kohrer, C.; RajBhandary, U. L.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 5, p. 2268-2273
Engineering Chemical Reactivity on Cell Surfaces Through Oligosaccharide Biosynthesis
journal, May 1997
- Mahal, Lara K.; Yarema, Kevin J.; Bertozzi, Carolyn R.
- Science, Vol. 276, Issue 5315, p. 1125-1128
Bio–incorporation of telluromethionine into buried residues of dihydrofolate reductase
journal, May 1994
- Boles, Jeffrey O.; Lewinski, Krzysztof; Kunkle, Marci
- Nature Structural & Molecular Biology, Vol. 1, Issue 5, p. 283-284
An unnatural base pair for incorporating amino acid analogs into proteins
journal, February 2002
- Hirao, Ichiro; Ohtsuki, Takashi; Fujiwara, Tsuyoshi
- Nature Biotechnology, Vol. 20, Issue 2
Site-specific incorporation of novel backbone structures into proteins
journal, January 1992
- Ellman, J.; Mendel, D.; Schultz, P.
- Science, Vol. 255, Issue 5041
An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system
journal, July 2002
- Kiga, D.; Sakamoto, K.; Kodama, K.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 15, p. 9715-9720
In vitro protein engineering using synthetic tRNAAla with different anticodons
journal, August 1993
- Ma, Chunhua; Kudlicki, Wieslaw; Odom, O. W.
- Biochemistry, Vol. 32, Issue 31
Direct Physical Measure of Conformational Rearrangement Underlying Potassium Channel Gating
journal, January 1996
- Mannuzzu, L. M.; Moronne, M. M.; Isacoff, E. Y.
- Science, Vol. 271, Issue 5246
An Expanded Eukaryotic Genetic Code
journal, August 2003
- Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
- Science, Vol. 301, Issue 5635, p. 964-967
Addition of p-Azido-L -phenylalanine to the Genetic Code of Escherichia coli
journal, July 2002
- Chin, Jason W.; Santoro, Stephen W.; Martin, Andrew B.
- Journal of the American Chemical Society, Vol. 124, Issue 31, p. 9026-9027
An efficient system for the evolution of aminoacyl-tRNA synthetase specificity
journal, September 2002
- Santoro, Stephen W.; Wang, Lei; Herberich, Brad
- Nature Biotechnology, Vol. 20, Issue 10
Modification of the amino acid specificity of tyrosyl-tRNA synthetase by protein engineering
journal, March 1993
- de Prat Gay, Gonzalo; Duckworth, Harry W.; Fersht, Alan R.
- FEBS Letters, Vol. 318, Issue 2, p. 167-171
Flash Decaging of Tyrosine Sidechains in an Ion Channel
journal, April 1998
- Miller, Jeffrey C.; Silverman, Scott K.; England, Pamela M.
- Neuron, Vol. 20, Issue 4, p. 619-624
Synthesis of 4-substituted prolines as conformationally constrained amino acid analogs
journal, April 1989
- Koskinen, Ari M. P.; Rapoport, Henry
- The Journal of Organic Chemistry, Vol. 54, Issue 8
Probing the Structure and Function of the Tachykinin Neurokinin-2 Receptor through Biosynthetic Incorporation of Fluorescent Amino Acids at Specific Sites
journal, August 1996
- Turcatti, Gerardo; Nemeth, Karin; Edgerton, Michael D.
- Journal of Biological Chemistry, Vol. 271, Issue 33
Selenocysteine: the 21st amino acid
journal, March 1991
- Böck, A.; Forchhammer, K.; Heider, J.
- Molecular Microbiology, Vol. 5, Issue 3
Relaxing the substrate specificity of an aminoacyl-tRNA synthetase allows in vitro and in vivo synthesis of proteins containing unnatural amino acids
journal, May 1995
- Ibba, Michael; Hennecke, Hauke
- FEBS Letters, Vol. 364, Issue 3, p. 272-275
A set of plasmids constitutively producing different RNA levels in Escherichia coli
journal, July 1999
- Gabriel, Kay; McClain, William H.
- Journal of Molecular Biology, Vol. 290, Issue 2
Efforts toward Expansion of the Genetic Alphabet: Replication of DNA with Three Base Pairs
journal, August 2001
- Tae, Eunju Lee; Wu, Yiqin; Xia, Gang
- Journal of the American Chemical Society, Vol. 123, Issue 30
Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle.
journal, November 1986
- Krieg, U. C.; Walter, P.; Johnson, A. E.
- Proceedings of the National Academy of Sciences, Vol. 83, Issue 22
Adding l-3-(2-Naphthyl)alanine to the Genetic Code of E. coli
journal, March 2002
- Wang, Lei; Brock, Ansgar; Schultz, Peter G.
- Journal of the American Chemical Society, Vol. 124, Issue 9, p. 1836-1837
Selenomethionyl proteins produced for analysis by multiwavelength anomalous diffraction (MAD): a vehicle for direct determination of three-dimensional structure.
journal, May 1990
- Hendrickson, W. A.; Horton, J. R.; LeMaster, D. M.
- The EMBO Journal, Vol. 9, Issue 5
Evolutionary relationship between Halobacterium cutirubrum and eukaryotes determined by use of aminoacyl-tRNA synthetases as phylogenetic probes
journal, March 1980
- Kwok, Yau; Wong, J. Tze-Fei
- Canadian Journal of Biochemistry, Vol. 58, Issue 3
[15] Biosynthetic method for introducing unnatural amino acids site-specifically into proteins
book, January 1991
- Ellman, Jon; Mendel, David; Anthony-Cahill, Spencer
- Molecular Design and Modeling: Concepts and Applications Part A: Proteins, Peptides, and Enzymes, p. 301-336
Human Tyrosyl-tRNA Synthetase Shares Amino Acid Sequence Homology with a Putative Cytokine
journal, May 1997
- Kleeman, Theresa A.; Wei, Dongbing; Simpson, Keith L.
- Journal of Biological Chemistry, Vol. 272, Issue 22
Expanding tRNA recognition of a tRNA synthetase by a single amino acid change
journal, May 2003
- Feng, L.; Tumbula-Hansen, D.; Toogood, H.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 10, p. 5676-5681
Rational Design of an Unnatural Base Pair with Increased Kinetic Selectivity
journal, September 2000
- Ogawa, Anthony K.; Wu, Yiqin; Berger, Markus
- Journal of the American Chemical Society, Vol. 122, Issue 36, p. 8803-8804
Unnatural amino acids as probes of protein structure and function
journal, December 2000
- Dougherty, D.
- Current Opinion in Chemical Biology, Vol. 4, Issue 6, p. 645-652
TRNA2Gln Su+2 mutants that increase amber suppression.
journal, January 1981
- Bradley, D.; Park, J. V.; Soll, L.
- Journal of Bacteriology, Vol. 145, Issue 2
Quadruplet codons: implications for code expansion and the specification of translation step size
journal, April 2000
- Moore, Barry; Persson, Britt C.; Nelson, Chad C.
- Journal of Molecular Biology, Vol. 298, Issue 2
Progress toward the evolution of an organism with an expanded genetic code
journal, April 1999
- Liu, David R.; Schultz, Peter G.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 9, p. 4780-4785
Enzymatic incorporation of a new base pair into DNA and RNA extends the genetic alphabet
journal, January 1990
- Piccirilli, Joseph A.; Benner, Steven A.; Krauch, Tilman
- Nature, Vol. 343, Issue 6253
Construction of Escherichia coli amber suppressor tRNA genes: II. Synthesis of additional tRNA genes and improvement of suppressor efficiency
journal, June 1990
- Kleina, Lynn G.; Masson, Jean-Michel; Normanly, Jennifer
- Journal of Molecular Biology, Vol. 213, Issue 4, p. 705-717
Editing of errors in selection of amino acids for protein synthesis.
journal, January 1992
- Jakubowski, H.; Goldman, E.
- Microbiological Reviews, Vol. 56, Issue 3
Genetic code in evolution: switching species-specific aminoacylation with a peptide transplant
journal, January 1998
- Wakasugi, K.
- The EMBO Journal, Vol. 17, Issue 1
Incorporation of Trifluoromethionine into a Phage Lysozyme: Implications and a New Marker for Use in Protein 19 F NMR †
journal, March 1997
- Duewel, Henry; Daub, Elisabeth; Robinson, Valerie
- Biochemistry, Vol. 36, Issue 11
An Engineered Tetrahymena tRNA Gln for in Vivo Incorporation of Unnatural Amino Acids into Proteins by Nonsense Suppression
journal, September 1996
- Saks, Margaret E.; Sampson, Jeffrey R.; Nowak, Mark W.
- Journal of Biological Chemistry, Vol. 271, Issue 38
Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
journal, July 1998
- Griffin, B. Albert; Adams, Stephen R.; Tsien, Roger Y.
- Science, Vol. 281, Issue 5374, p. 269-272
2 .ANG. Resolution Structure of DppA, a Periplasmic Dipeptide Transport/Chemosensory Receptor
journal, December 1995
- Nickitenko, Alexei V.; Trakhanov, Sergei; Quiocho, Florante A.
- Biochemistry, Vol. 34, Issue 51
Enzyme-generated intracellular fluorescence for single-cell reporter gene analysis utilizing Escherichia coli-glucuronidase
journal, August 1996
- Lorincz, Matthew; Roederer, Mario; Diwu, Zhenjun
- Cytometry, Vol. 24, Issue 4, p. 321-329
Biodegradation of Aromatic Compounds by Escherichia coli
journal, December 2001
- Diaz, E.; Ferrandez, A.; Prieto, M. A.
- Microbiology and Molecular Biology Reviews, Vol. 65, Issue 4
Differences in Backbone Structure between Angiotensin II Agonists and Type I Antagonists
journal, November 1995
- Matsoukas, John M.; Agelis, George; Wahhab, Amal
- Journal of Medicinal Chemistry, Vol. 38, Issue 23
Engineering a tRNA and aminoacyl-tRNA synthetase for the site-specific incorporation of unnatural amino acids into proteins in vivo
journal, September 1997
- Liu, D. R.; Magliery, T. J.; Pastrnak, M.
- Proceedings of the National Academy of Sciences, Vol. 94, Issue 19
Protein Engineering by In Vivo Incorporation of Non-Natural Amino Acids: Control of Incorporation of Methionine Analogues by Methionyl-tRNA Synthetase
journal, November 2000
- Kiick, Kristi L.; Tirrell, David A.
- Tetrahedron, Vol. 56, Issue 48, p. 9487-9493
Enlarging the Amino Acid Set of Escherichia coli by Infiltration of the Valine Coding Pathway
journal, April 2001
- Doring, V.; Mootz, H. D.; Nangle, L. A.
- Science, Vol. 292, Issue 5516
Site-Directed Mutagenesis with an Expanded Genetic Code
journal, June 1995
- Mendel, D.; Cornish, V. W.; Schultz, P. G.
- Annual Review of Biophysics and Biomolecular Structure, Vol. 24, Issue 1
A synthetic IgG-binding domain based on staphylococcal protein A
journal, January 1987
- Nilsson, Björn; Moks, Tomas; Jansson, Birger
- "Protein Engineering, Design and Selection", Vol. 1, Issue 2
Incorporation of Two Different Nonnatural Amino Acids Independently into a Single Protein through Extension of the Genetic Code
journal, December 1999
- Hohsaka, Takahiro; Ashizuka, Yuki; Sasaki, Hiroshi
- Journal of the American Chemical Society, Vol. 121, Issue 51
Co-Expression of Yeast Amber Suppressor tRNATyr and Tyrosyl-tRNA Synthetase in Escherichia coli: Possibility to Expand the Genetic Code
journal, December 1998
- Ohno, S.; Yokogawa, T.; Fujii, I.
- Journal of Biochemistry, Vol. 124, Issue 6
Substrate Specificity Is Determined by Amino Acid Binding Pocket Size in Escherichia coli Phenylalanyl-tRNA Synthetase
journal, June 1994
- Ibba, Michael; Kast, Peter; Hennecke, Hauke
- Biochemistry, Vol. 33, Issue 23
Biosynthetic site-specific incorporation of a non-natural amino acid into a polypeptide
journal, September 1989
- Bain, J. D.; Diala, Edward S.; Glabe, Charles G.
- Journal of the American Chemical Society, Vol. 111, Issue 20, p. 8013-8014
An expanding genetic code
journal, December 2004
- Cropp, T. Ashton; Schultz, Peter G.
- Trends in Genetics, Vol. 20, Issue 12, p. 625-630
Expanding the Genetic Code
journal, January 2005
- Wang, Lei; Schultz, Peter G.
- Angewandte Chemie International Edition, Vol. 44, Issue 1, p. 34-66
Residue-specific bioincorporation of non-natural, biologically active amino acids into proteins as possible drug carriers: Structure and stability of the per-thiaproline mutant of annexin V
journal, January 1998
- Budisa, N.; Minks, C.; Medrano, F. J.
- Proceedings of the National Academy of Sciences, Vol. 95, Issue 2
Synthetically modified DNAs as substrates for polymerases
journal, December 2000
- Kool, E.
- Current Opinion in Chemical Biology, Vol. 4, Issue 6, p. 602-608
Programming peptidomimetic syntheses by translating genetic codes designed de novo
journal, May 2003
- Forster, A. C.; Tan, Z.; Nalam, M. N. L.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 11, p. 6353-6357
Multicolor and Electron Microscopic Imaging of Connexin Trafficking
journal, April 2002
- Gaietta, G.
- Science, Vol. 296, Issue 5567
New Photolabeling and Crosslinking Methods
journal, June 1993
- Brunner, Josef
- Annual Review of Biochemistry, Vol. 62, Issue 1
Probing ion permeation and gating in a K+ channel with backbone mutations in the selectivity filter
journal, March 2001
- Lu, Tao; Ting, Alice Y.; Mainland, Joel
- Nature Neuroscience, Vol. 4, Issue 3
Transmembrane protein structure: spin labeling of bacteriorhodopsin mutants
journal, June 1990
- Altenbach, C.; Marti, T.; Khorana, H.
- Science, Vol. 248, Issue 4959
Universal rules and idiosyncratic features in tRNA identity
journal, November 1998
- Giege, R.; Sissler, M.; Florentz, C.
- Nucleic Acids Research, Vol. 26, Issue 22
Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme
journal, July 1998
- Jeruzalmi, David; Steitz, Thomas A.
- The EMBO Journal, Vol. 17, Issue 14
Absolute configuration of the enantiomers of 7-chloro-4-[[4-(diethylamino)-1-methylbutyl]amino]quinoline (chloroquine)
journal, March 1988
- Craig, J. Cymerman; Bhargava, Hemendra N.; Everhart, E. Thomas
- The Journal of Organic Chemistry, Vol. 53, Issue 6, p. 1167-1170
Novel cofactors via post-translational modifications of enzyme active sites
journal, July 2000
- Okeley, Nicole M.; van der Donk, Wilfred A.
- Chemistry & Biology, Vol. 7, Issue 7
Site-Specific Protein Modification Using a Ketone Handle
journal, January 1996
- Cornish, Virginia W.; Hahn, Klaus M.; Schultz, Peter G.
- Journal of the American Chemical Society, Vol. 118, Issue 34, p. 8150-8151
Nicotinic receptor binding site probed with unnatural amino acid incorporation in intact cells
journal, April 1995
- Nowak, M.; Kearney, P.
- Science, Vol. 268, Issue 5209
Probing Protein Structure and Function with an Expanded Genetic Code
journal, March 1995
- Cornish, Virginia W.; Mendel, David; Schultz, Peter G.
- Angewandte Chemie International Edition in English, Vol. 34, Issue 6, p. 621-633
Directed evolution of the site specificity of Cre recombinase
journal, March 2002
- Santoro, S. W.; Schultz, P. G.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 7, p. 4185-4190
Studying organelle physiology with fusion protein-targeted avidin and fluorescent biotin conjugates
book, January 2000
- Wu, Minnie M.; Llopis, Juan; Adams, Stephen R.
- Methods in Enzymology
Site-directed conjugation of nonpeptide groups to peptides and proteins via periodate oxidation of a 2-amino alcohol. Application to modification at N-terminal serine
journal, March 1992
- Geoghegan, Kieran F.; Stroh, Justin G.
- Bioconjugate Chemistry, Vol. 3, Issue 2
A New Strategy for the Site-Specific Modification of Proteins in Vivo
journal, June 2003
- Zhang, Zhiwen; Smith, Brian A. C.; Wang, Lei
- Biochemistry, Vol. 42, Issue 22, p. 6735-6746
Highly Precise Shape Mimicry by a Difluorotoluene Deoxynucleoside, a Replication-Competent Substitute for Thymidine
journal, January 1997
- Guckian, Kevin M.; Kool, Eric T.
- Angewandte Chemie International Edition in English, Vol. 36, Issue 24, p. 2825-2828
Site-specific incorporation of biotinylated amino acids to identify surface-exposed residues in integral membrane proteins
journal, October 1997
- Gallivan, Justin P.; Lester, Henry A.; Dougherty, Dennis A.
- Chemistry & Biology, Vol. 4, Issue 10, p. 739-749
A general approach for the generation of orthogonal tRNAs
journal, September 2001
- Wang, Lei; Schultz, Peter G.
- Chemistry & Biology, Vol. 8, Issue 9, p. 883-890
Structure of tyrosyl-tRNA synthetase refined at 2.3 Å resolution: Interaction of the enzyme with the tyrosyl adenylate intermediate
journal, July 1989
- Brick, P.; Bhat, T. N.; Blow, D. M.
- Journal of Molecular Biology, Vol. 208, Issue 1, p. 83-98
Major Anticodon-binding Region Missing from an Archaebacterial tRNA Synthetase
journal, December 1999
- Steer, Brian A.; Schimmel, Paul
- Journal of Biological Chemistry, Vol. 274, Issue 50
A Novel Copper-Mediated DNA Base Pair
journal, November 2000
- Meggers, Eric; Holland, Patrick L.; Tolman, William B.
- Journal of the American Chemical Society, Vol. 122, Issue 43, p. 10714-10715
Backbone Mutations in Transmembrane Domains of a Ligand-Gated Ion Channel
journal, January 1999
- England, Pamela M.; Zhang, Yinong; Dougherty, Dennis A.
- Cell, Vol. 96, Issue 1, p. 89-98
Strategies for in vitro and in vivo translation with non-natural amino acids
journal, December 1996
- Ibba, Michael
- Biotechnology and Genetic Engineering Reviews, Vol. 13, Issue 1
A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins
journal, May 2000
- Wang, Lei; Magliery, Thomas J.; Liu, David R.
- Journal of the American Chemical Society, Vol. 122, Issue 20, p. 5010-5011
Efforts toward Expansion of the Genetic Alphabet: DNA Polymerase Recognition of a Highly Stable, Self-Pairing Hydrophobic Base
journal, December 1999
- McMinn, Dustin L.; Ogawa, Anthony K.; Wu, Yiqin
- Journal of the American Chemical Society, Vol. 121, Issue 49
Quisqualic acid analogs: synthesis of .beta.-heterocyclic 2-aminopropanoic acid derivatives and their activity at a novel quisqualate-sensitized site
journal, November 1992
- Subasinghe, Nalin; Schulte, Marvin; Roon, Robert J.
- Journal of Medicinal Chemistry, Vol. 35, Issue 24
Noninvasive Tracing of Recombinant Proteins with “Fluorophenylalanine-Fingers”
journal, August 2000
- Minks, Caroline; Huber, Robert; Moroder, Luis
- Analytical Biochemistry, Vol. 284, Issue 1
Libraries of hybrid proteins from distantly related sequences
journal, May 2001
- Sieber, Volker; Martinez, Carlos A.; Arnold, Frances H.
- Nature Biotechnology, Vol. 19, Issue 5
5′–3′ Exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis
journal, January 1988
- Sayers, Jon R.; Schmidt, Walter; Eckstein, Fritz
- Nucleic Acids Research, Vol. 16, Issue 3
Compilation of tRNA sequences and sequences of tRNA genes
journal, January 1998
- Sprinzl, Mathias; Horn, Carsten; Brown, Melissa
- Nucleic Acids Research, Vol. 26, Issue 1, p. 148-153
Glycine tRNA mutants with normal anticodon loop size cause -1 frameshifting.
journal, October 1989
- O'Mahony, D. J.; Mims, B. H.; Thompson, S.
- Proceedings of the National Academy of Sciences, Vol. 86, Issue 20
A general method for site-specific incorporation of unnatural amino acids into proteins
journal, April 1989
- Noren, C.; Anthony-Cahill, S.; Griffith, M.
- Science, Vol. 244, Issue 4901
Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002
- Wang, L.; Zhang, Z.; Brock, A.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 1, p. 56-61
Pyrrolysine Encoded by UAG in Archaea: Charging of a UAG-Decoding Specialized tRNA
journal, May 2002
- Srinivasan, G.
- Science, Vol. 296, Issue 5572
Enzymatic Phosphorylation of Unnatural Nucleosides
journal, December 2002
- Wu, Yiqin; Fa, Ming; Tae, Eunju Lee
- Journal of the American Chemical Society, Vol. 124, Issue 49
Bioincorporation of telluromethionine into proteins: a promising new approach for X-ray structure analysis of proteins
journal, July 1997
- Budisa, Nediljko; Karnbrock, Wilhelm; Steinbacher, Stefan
- Journal of Molecular Biology, Vol. 270, Issue 4, p. 616-623
Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface.
journal, November 1993
- Francisco, J. A.; Campbell, R.; Iverson, B. L.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 22
Efficient introduction of alkene functionality into proteins in vivo
journal, May 1998
- van Hest, Jan C. M.; Tirrell, David A.
- FEBS Letters, Vol. 428, Issue 1-2, p. 68-70
Enzymatic incorporation of a new base pair into DNA and RNA
journal, October 1989
- Switzer, Christopher; Moroney, Simon E.; Benner, Steven A.
- Journal of the American Chemical Society, Vol. 111, Issue 21, p. 8322-8323
Major tyrosine identity determinants in Methanococcus jannaschii and Saccharomyces cerevisiae tRNA Tyr are conserved but expressed differently : tRNA identity for
journal, February 2001
- Fechter, Pierre; Rudinger-Thirion, Joëlle; Tukalo, Mikhail
- European Journal of Biochemistry, Vol. 268, Issue 3
Fluorinated Coiled-Coil Proteins Prepared In Vivo Display Enhanced Thermal and Chemical Stability
journal, April 2001
- Tang, Yi; Ghirlanda, Giovanna; Petka, Wendy A.
- Angewandte Chemie International Edition, Vol. 40, Issue 8, p. 1494-1496
693. A new synthesis of glutamine and of γ-dipeptides of glutamic acid from phthalylated intermediates
journal, January 1949
- King, F. E.; Kidd, D. A. A.
- J. Chem. Soc., Vol. 0, Issue 0
Toward the experimental codon reassignment in vivo : protein building with an expanded amino acid repertoire
journal, January 1999
- Budisa, Nediljko; Minks, Caroline; Alefelder, Stefan
- The FASEB Journal, Vol. 13, Issue 1
Exploring the Limits of Codon and Anticodon Size
journal, February 2002
- Anderson, J. Christopher; Magliery, Thomas J.; Schultz, Peter G.
- Chemistry & Biology, Vol. 9, Issue 2, p. 237-244
Crystal structure of the dipeptide binding protein from Escherichia coli involved in active transport and chemotaxis
journal, November 1995
- Dunten, Pete; Mowbray, Sherry L.
- Protein Science, Vol. 4, Issue 11
Efficient introduction of aryl bromide functionality into proteins in vivo
journal, February 2000
- Sharma, Nandita; Furter, Rolf; Kast, Peter
- FEBS Letters, Vol. 467, Issue 1, p. 37-40
Fluorescent Protein Biosensors: Measurement of Molecular Dynamics in Living Cells
journal, June 1995
- Giuliano, K. A.; Post, P. L.; Hahn, K. M.
- Annual Review of Biophysics and Biomolecular Structure, Vol. 24, Issue 1
Protein-protein interaction: a genetic selection for compensating mutations at the barnase-barstar interface.
journal, March 1996
- Jucovic, M.; Hartley, R. W.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 6
Phage selection for site-specific incorporation of unnatural amino acids into proteins in vivo
journal, September 2001
- Pastrnak, M.
- Bioorganic & Medicinal Chemistry, Vol. 9, Issue 9, p. 2373-2379
Quantitation of Transcription and Clonal Selection of Single Living Cells with -Lactamase as Reporter
journal, January 1998
- Zlokarnik, G.
- Science, Vol. 279, Issue 5347
A New UAG-Encoded Residue in the Structure of a Methanogen Methyltransferase
journal, May 2002
- Hao, B.
- Science, Vol. 296, Issue 5572
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002
- Chin, J. W.; Martin, A. B.; King, D. S.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 17, p. 11020-11024
Efficient Incorporation of Nonnatural Amino Acids with Large Aromatic Groups into Streptavidin in In Vitro Protein Synthesizing Systems
journal, January 1999
- Hohsaka, Takahiro; Kajihara, Daisuke; Ashizuka, Yuki
- Journal of the American Chemical Society, Vol. 121, Issue 1
Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli
journal, March 2001
- Magliery, Thomas J.; Anderson, J. Christopher; Schultz, Peter G.
- Journal of Molecular Biology, Vol. 307, Issue 3, p. 755-769
Synthesis of novel α-amino-acids and derivatives using radical chemistry: synthesis of L- and D-α-amino-adipic acids, L-α
journal, January 1987
- Barton, Derek H. R.; Hervé, Yolande; Potier, Pierre
- Tetrahedron, Vol. 43, Issue 19, p. 4297-4308
Aminoacyl-tRNA synthetases: Versatile players in the changing theater of translation
journal, November 2002
- Francklyn, Christopher; Perona, John J.; Puetz, Joern
- RNA, Vol. 8, Issue 11
High-level Biosynthetic Substitution of Methionine in Proteins by its Analogs 2-Aminohexanoic Acid, Selenomethionine, Telluromethionine and Ethionine in Escherichia coli
journal, June 1995
- Budisa, Nediljko; Steipe, Boris; Demange, Pascal
- European Journal of Biochemistry, Vol. 230, Issue 2
A binding protein for l-glutamine and its relation to active transport in E. coli
journal, February 1971
- Weiner, Joel H.; Furlong, Clement E.; Heppel, Leon A.
- Archives of Biochemistry and Biophysics, Vol. 142, Issue 2, p. 715-717
Novel Biological Process for L-DOPA Production from L-Tyrosine by P-Hydroxyphenylacetate 3-Hydroxylase
journal, January 1998
- Lee, Jang-Young; Xun, Luying
- Biotechnology Letters, Vol. 20, Issue 5, p. 479-482
[5]Disulfide bonds as probes of protein folding pathways
book, January 1986
- Creighton, Thomas E.
- Methods in Enzymology
A Mutant Escherichia coli Tyrosyl-tRNA Synthetase Utilizes the Unnatural Amino Acid Azatyrosine More Efficiently than Tyrosine
journal, September 2000
- Hamano-Takaku, Fumie; Iwama, Toshiharu; Saito-Yano, Shio
- Journal of Biological Chemistry, Vol. 275, Issue 51, p. 40324-40328
Expanding the genetic code
journal, December 2001
- Wang, Lei; Schultz, Peter G.
- Chemical Communications, Issue 1
Efficient Incorporation of Unsaturated Methionine Analogues into Proteins in Vivo
journal, February 2000
- van Hest, Jan C. M.; Kiick, Kristi L.; Tirrell, David A.
- Journal of the American Chemical Society, Vol. 122, Issue 7
Translational efficiency of transfer RNA's: uses of an extended anticodon
journal, November 1982
- Yarus, M.
- Science, Vol. 218, Issue 4573
Synthesis of optically pure pipecolates from L-asparagine. Application to the total synthesis of (+)-apovincamine through amino acid decarbonylation and iminium ion cyclization
journal, April 1985
- Christie, Bradley D.; Rapoport, Henry
- The Journal of Organic Chemistry, Vol. 50, Issue 8
Aspartate identity of transfer RNAs
journal, January 1996
- Giegé, R.; Florentz, C.; Kern, D.
- Biochimie, Vol. 78, Issue 7, p. 605-623
Characterization of an ‘orthogonal’ suppressor tRNA derived from E. coli tRNA2Gln
journal, September 1997
- Liu, David R.; Magliery, Thomas J.; Schultz, Peter G.
- Chemistry & Biology, Vol. 4, Issue 9, p. 685-691
The gene cluster for chloramphenicol biosynthesis in Streptomyces venezuelae ISP5230 includes novel shikimate pathway homologues and a monomodular non-ribosomal peptide synthetase gene
journal, October 2001
- Piraee, M.; Magarvey, N.; He, J.
- Microbiology, Vol. 147, Issue 10
Efforts toward the Expansion of the Genetic Alphabet: Information Storage and Replication with Unnatural Hydrophobic Base Pairs
journal, April 2000
- Ogawa, Anthony K.; Wu, Yiqin; McMinn, Dustin L.
- Journal of the American Chemical Society, Vol. 122, Issue 14
Glutamine analogues as potential antimalarials
journal, March 1991
- Azoulay, M.; Vilmont, M.; Frappier, F.
- European Journal of Medicinal Chemistry, Vol. 26, Issue 2, p. 201-205
Crystal structure of yeast phenylalanine transfer RNA: I. Crystallographic refinement
journal, August 1978
- Sussman, Joel L.; Holbrook, Stephen R.; Warrant, R. Wade
- Journal of Molecular Biology, Vol. 123, Issue 4, p. 607-630
Exploiting unassigned codons in Micrococcus luteus for tRNA-based amino acid mutagenesis
journal, November 1997
- Kowal, A.
- Nucleic Acids Research, Vol. 25, Issue 22
Synthesis of Derivatives of Glutamine as Model Substrates for Anti-tumor Agents
journal, July 1959
- Friedman, Orrie M.; Chatterji, Ramesh
- Journal of the American Chemical Society, Vol. 81, Issue 14, p. 3750-3752
Expanding the Genetic Code of Escherichia coli
journal, April 2001
- Wang, L.; Brock, A.; Herberich, B.
- Science, Vol. 292, Issue 5516, p. 498-500
Rescuing an essential enzyme-RNA complex with a non-essential appended domain
journal, May 1997
- Whelihan, E. F.
- The EMBO Journal, Vol. 16, Issue 10
Expansion of the genetic code: Site-directed p-fluoro-phenylalanine incorporation in Escherichia coli
journal, February 1998
- Furter, Rolf
- Protein Science, Vol. 7, Issue 2, p. 419-426