Microbial reductive dehalogenation of vinyl chloride
Abstract
Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by Dehalococcoides-like organisms. An isolated strain of bacteria, Dehalococcoides sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.
- Inventors:
-
- Stanford, CA
- Baltimore, MD
- Berlin, DE
- Nannhein, DE
- Los Altos, CA
- Issue Date:
- Research Org.:
- Stanford Univ., CA (United States)
- Sponsoring Org.:
- USDOE Office of Environmental Management (EM)
- OSTI Identifier:
- 1032875
- Patent Number(s):
- 8063192
- Application Number:
- 11/659,064
- Assignee:
- The Board of Trustees of the Leland Stanford Junior University (Palo Alto, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B09 - DISPOSAL OF SOLID WASTE B09C - RECLAMATION OF CONTAMINATED SOIL
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG07-99ER62883
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Spormann, Alfred M, Muller, Jochen A, Rosner, Bettina M, Von Abendroth, Gregory, Meshulam-Simon, Galit, and McCarty, Perry L. Microbial reductive dehalogenation of vinyl chloride. United States: N. p., 2011.
Web.
Spormann, Alfred M, Muller, Jochen A, Rosner, Bettina M, Von Abendroth, Gregory, Meshulam-Simon, Galit, & McCarty, Perry L. Microbial reductive dehalogenation of vinyl chloride. United States.
Spormann, Alfred M, Muller, Jochen A, Rosner, Bettina M, Von Abendroth, Gregory, Meshulam-Simon, Galit, and McCarty, Perry L. Tue .
"Microbial reductive dehalogenation of vinyl chloride". United States. https://www.osti.gov/servlets/purl/1032875.
@article{osti_1032875,
title = {Microbial reductive dehalogenation of vinyl chloride},
author = {Spormann, Alfred M and Muller, Jochen A and Rosner, Bettina M and Von Abendroth, Gregory and Meshulam-Simon, Galit and McCarty, Perry L},
abstractNote = {Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by Dehalococcoides-like organisms. An isolated strain of bacteria, Dehalococcoides sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 22 00:00:00 EST 2011},
month = {Tue Nov 22 00:00:00 EST 2011}
}
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Works referenced in this record:
From genes to protein structure and function: novel applications of computational approaches in the genomic era
journal, January 2000
- Skolnick, Jeffrey; Fetrow, Jacquelyn S.
- Trends in Biotechnology, Vol. 18, Issue 1, p. 34-39
Trichloroethene Reductive Dehalogenase from Dehalococcoides ethenogenes: Sequence of tceA and Substrate Range Characterization
journal, December 2000
- Magnuson, J. K.; Romine, M. F.; Burris, D. R.
- Applied and Environmental Microbiology, Vol. 66, Issue 12
Detoxification of vinyl chloride to ethene coupled to growth of an anaerobic bacterium
journal, July 2003
- He, Jianzhong; Ritalahti, Kirsti M.; Yang, Kun-Lin
- Nature, Vol. 424, Issue 6944
Growth of a Dehalococcoides-Like Microorganism on Vinyl Chloride and cis-Dichloroethene as Electron Acceptors as Determined by Competitive PCR
journal, February 2003
- Cupples, A. M.; Spormann, A. M.; McCarty, P. L.
- Applied and Environmental Microbiology, Vol. 69, Issue 2
Reductive Dechlorination of cis -1,2-Dichloroethene and Vinyl Chloride by “ Dehalococcoides ethenogenes ”
journal, February 2001
- Maymó-Gatell, Xavier; Nijenhuis, Ivonne; Zinder, Stephen H.
- Environmental Science & Technology, Vol. 35, Issue 3
Reductive Dechlorination of Tetrachloroethene to Ethene by a Two-Component Enzyme Pathway
journal, April 1998
- Magnuson, Jon K.; Stern, Robert V.; Gossett, James M.
- Applied and Environmental Microbiology, Vol. 64, Issue 4
Isolation of a Bacterium That Reductively Dechlorinates Tetrachloroethene to Ethene
journal, June 1997
- Maymo-Gatell, X.
- Science, Vol. 276, Issue 5318
Complete Detoxification of Vinyl Chloride by an Anaerobic Enrichment Culture and Identification of the Reductively Dechlorinating Population as a Dehalococcoides Species
journal, February 2003
- He, J.; Ritalahti, K. M.; Aiello, M. R.
- Applied and Environmental Microbiology, Vol. 69, Issue 2
Molecular Analysis of Dehalococcoides 16S Ribosomal DNA from Chloroethene-Contaminated Sites throughout North America and Europe
journal, February 2002
- Hendrickson, E. R.; Payne, J. A.; Young, R. M.
- Applied and Environmental Microbiology, Vol. 68, Issue 2
In vitro studies on reductive vinyl chloride dehalogenation by an anaerobic mixed culture.
journal, January 1997
- Rosner, B. M.; McCarty, P. L.; Spormann, A. M.
- Applied and environmental microbiology, Vol. 63, Issue 11