Zymomonas with improved xylose utilization
Abstract
Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.
- Inventors:
-
- West Chester, PA
- Havertown, PA
- New Hope, PA
- Kennett Square, PA
- Wilmington, DE
- East Fallowfield, PA
- Lakewood, CO
- Centennial, CO
- Issue Date:
- Research Org.:
- E. I. du Pont de Nemours and Company (Wilmington, DE)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1025535
- Patent Number(s):
- 7998722
- Application Number:
- 12/410,501
- Assignee:
- E. I. du Pont de Nemours and Company (Wilmington, DE); Alliance for Sustainable Energy LLC (Wilmington, DE)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FC36-03GO13146
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Viitanen, Paul V, Tao, Luan, Zhang, Yuying, Caimi, Perry G, McCutchen, Carol M, McCole, Laura, Zhang, Min, Chou, Yat-Chen, and Franden, Mary Ann. Zymomonas with improved xylose utilization. United States: N. p., 2011.
Web.
Viitanen, Paul V, Tao, Luan, Zhang, Yuying, Caimi, Perry G, McCutchen, Carol M, McCole, Laura, Zhang, Min, Chou, Yat-Chen, & Franden, Mary Ann. Zymomonas with improved xylose utilization. United States.
Viitanen, Paul V, Tao, Luan, Zhang, Yuying, Caimi, Perry G, McCutchen, Carol M, McCole, Laura, Zhang, Min, Chou, Yat-Chen, and Franden, Mary Ann. Tue .
"Zymomonas with improved xylose utilization". United States. https://www.osti.gov/servlets/purl/1025535.
@article{osti_1025535,
title = {Zymomonas with improved xylose utilization},
author = {Viitanen, Paul V and Tao, Luan and Zhang, Yuying and Caimi, Perry G and McCutchen, Carol M and McCole, Laura and Zhang, Min and Chou, Yat-Chen and Franden, Mary Ann},
abstractNote = {Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 16 00:00:00 EDT 2011},
month = {Tue Aug 16 00:00:00 EDT 2011}
}
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