Title: Systems for the expression of orthogonal translation components in eubacterial host cells

Abstract

The invention relates to compositions and methods for the in vivo production of polypeptides comprising one or more unnatural amino acids. Specifically, the invention provides plasmid systems for the efficient eubacterial expression of polypeptides comprising one or more unnatural amino acids at genetically-programmed positions.

Inventors:

Ryu, Youngha ^[1]; Schultz, Peter G ^[2]

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1. San Diego, CA
2. La Jolla, CA

Issue Date:

Tue Jun 14 00:00:00 EDT 2011

Research Org.:

The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1021889

Patent Number(s):

7960141

Application Number:

11/715,672

Assignee:

The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/245 - Escherichia

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/70 - Vectors or expression systems specially adapted for E. coli

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P21/02 - having a known sequence of two or more amino acids, e.g. glutathione

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DOE Contract Number:  

FG02-03ER46051

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES