Highly thermostable fluorescent proteins
Abstract
Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.
- Inventors:
-
- Santa Fe, NM
- Los Alamos, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1016159
- Patent Number(s):
- 7910700
- Application Number:
- US Patent Application 12/317,185
- Assignee:
- Los Alamos National Security, LLC (Los Alamos, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- AC52-06NA25396
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Bradbury, Andrew M, Waldo, Geoffrey S, and Kiss, Csaba. Highly thermostable fluorescent proteins. United States: N. p., 2011.
Web.
Bradbury, Andrew M, Waldo, Geoffrey S, & Kiss, Csaba. Highly thermostable fluorescent proteins. United States.
Bradbury, Andrew M, Waldo, Geoffrey S, and Kiss, Csaba. Tue .
"Highly thermostable fluorescent proteins". United States. https://www.osti.gov/servlets/purl/1016159.
@article{osti_1016159,
title = {Highly thermostable fluorescent proteins},
author = {Bradbury, Andrew M and Waldo, Geoffrey S and Kiss, Csaba},
abstractNote = {Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 22 00:00:00 EDT 2011},
month = {Tue Mar 22 00:00:00 EDT 2011}
}
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