Switchable photosystem-II designer algae for photobiological hydrogen production

Lee, James Weifu

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Title: Switchable photosystem-II designer algae for photobiological hydrogen production

Abstract

A switchable photosystem-II designer algae for photobiological hydrogen production. The designer transgenic algae includes at least two transgenes for enhanced photobiological H.sub.2 production wherein a first transgene serves as a genetic switch that can controls photosystem II (PSII) oxygen evolution and a second transgene encodes for creation of free proton channels in the algal photosynthetic membrane. In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (302), a inducible promoter (304), a PSII-iRNA sequence (306), a terminator (308), and a PCR reverse primer (310). In other embodiments, the PSII-iRNA sequence (306) is replaced with a CF.sub.1-iRNA sequence (312), a streptomycin-production gene (314), a targeting sequence (316) followed by a proton-channel producing gene (318), or a PSII-producing gene (320). In one embodiment, a photo-bioreactor and gas-product separation and utilization system produce photobiological H.sub.2 from the switchable PSII designer alga.

Inventors:

Lee, James Weifu ^[1]

Knoxville, TN

Issue Date:: Tue Jan 05 00:00:00 EST 2010

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1015190

Patent Number(s):: 7642405

Application Number:: 11/748,550

Assignee:: Lee, James Weifu (Knoxville, TN)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K14/405 - from algae

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/8257 - {for the production of primary gene products, e.g. pharmaceutical products, interferon}

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DOE Contract Number:: AC05-00OR22725

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Citation Formats


                    Lee, James Weifu. Switchable photosystem-II designer algae for photobiological hydrogen production.  United States: N. p., 2010. 
        Web.

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                    Lee, James Weifu. Switchable photosystem-II designer algae for photobiological hydrogen production.  United States.

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                    Lee, James Weifu. Tue .  
        "Switchable photosystem-II designer algae for photobiological hydrogen production".  United States.  https://www.osti.gov/servlets/purl/1015190.

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@article{osti_1015190,

  title        = {Switchable photosystem-II designer algae for photobiological hydrogen production},

  author       = {Lee, James Weifu},

  abstractNote = {A switchable photosystem-II designer algae for photobiological hydrogen production. The designer transgenic algae includes at least two transgenes for enhanced photobiological H.sub.2 production wherein a first transgene serves as a genetic switch that can controls photosystem II (PSII) oxygen evolution and a second transgene encodes for creation of free proton channels in the algal photosynthetic membrane. In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (302), a inducible promoter (304), a PSII-iRNA sequence (306), a terminator (308), and a PCR reverse primer (310). In other embodiments, the PSII-iRNA sequence (306) is replaced with a CF.sub.1-iRNA sequence (312), a streptomycin-production gene (314), a targeting sequence (316) followed by a proton-channel producing gene (318), or a PSII-producing gene (320). In one embodiment, a photo-bioreactor and gas-product separation and utilization system produce photobiological H.sub.2 from the switchable PSII designer alga.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Jan 05 00:00:00 EST 2010},

  month        = {Tue Jan 05 00:00:00 EST 2010}

}

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Works referenced in this record:

Functional Analysis of Photosystem II
book,

Ruffle, Stuart V.; Sayre, Richard T.
The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas
https://doi.org/10.1007/0-306-48204-5_16

Temperature effect on production of hydrogen and oxygen byChlamydomonas cold strain CCMP1619 and wild-type 137c: Scientific note
journal, September 1995

Lee, James W.; Blankinship, Stephen L.; Greenbaum, Elias
Applied Biochemistry and Biotechnology, Vol. 51-52, Issue 1
https://doi.org/10.1007/BF02933441

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
journal, February 1998

Fire, Andrew; Xu, SiQun; Montgomery, Mary K.
Nature, Vol. 391, Issue 6669
https://doi.org/10.1038/35888

Assembly of Photosystem II
book,

Erickson, Jeanne Marie
The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas
https://doi.org/10.1007/0-306-48204-5_15

Killing the messenger: short RNAs that silence gene expression
journal, June 2003

Dykxhoorn, Derek M.; Novina, Carl D.; Sharp, Phillip A.
Nature Reviews Molecular Cell Biology, Vol. 4, Issue 6
https://doi.org/10.1038/nrm1129

Photoinhibition in indica and japonica subspecies of rice (Oryza sativa) and their reciprocal F1 hybrids
journal, January 2001

Jiao, Demao; Ji, Benhua
Functional Plant Biology, Vol. 28, Issue 4
https://doi.org/10.1071/PP00089

Oxygen sensitivity of algal H2- production
journal, March 1997

Ghirardi, Maria L.; Togasaki, Robert K.; Seibert, Michael
Applied Biochemistry and Biotechnology, Vol. 63-65, Issue 1
https://doi.org/10.1007/BF02920420

Improvement of Photosynthetic CO₂ Fixation at High Light Intensity Through Reduction of Chlorophyll Antenna Size
journal, January 2002

Lee, James W.; Mets, Laurens; Greenbaum, Elias
Applied Biochemistry and Biotechnology, Vol. 98-100, Issue 1-9
https://doi.org/10.1385/ABAB:98-100:1-9:37

Solar Photobiochemistry: Simultaneous Photoproduction of Hydrogen and Oxygen in a Confined Bioreactor
journal, May 2001

Greenbaum, Elias; Blankinship, Stephen L.; Lee, James W.
The Journal of Physical Chemistry B, Vol. 105, Issue 17
https://doi.org/10.1021/jp0042821

Oxygen Tolerance of the H ₂ -sensing [NiFe] Hydrogenase from Ralstonia eutropha H16 Is Based on Limited Access of Oxygen to the Active Site
journal, April 2005

Buhrke, Thorsten; Lenz, Oliver; Krauss, Norbert
Journal of Biological Chemistry, Vol. 280, Issue 25
https://doi.org/10.1074/jbc.M503260200

Cloning of a streptomycin-production gene directing synthesis of N-methyl-l-glucosamine
journal, January 1986

Yoichi, Kumada; Sueharu, Horinouchi; Takeshi, Uozumi
Gene, Vol. 42, Issue 2, p. 221-224
https://doi.org/10.1016/0378-1119(86)90300-8

A New Oxygen Sensitivity and Its Potential Application in Photosynthetic H2 Production
book, January 2003

Lee, James W.; Greenbaum, Elias
Biotechnology for Fuels and Chemicals
https://doi.org/10.1007/978-1-4612-0057-4_25

Functional and Structural Organization of Chlorophyll in the Developing Photosynthetic Membranes of Euglena gracilis Z: II. CHARACTERISTICS OF THE LATE FORMATION OF ACTIVE PHOTOSYSTEM II REACTION CENTERS DURING FIRST STAGES OF GREENING
journal, January 1981

Dubertret, Guy; Ambard-Bretteville, Françoise
Plant Physiology, Vol. 67, Issue 1
https://doi.org/10.1104/pp.67.1.47

Coordinate Copper- and Oxygen-responsive Cyc6 and Cpx1 Expression in Chlamydomonas Is Mediated by the Same Element
journal, February 2000

Quinn, Jeanette M.; Barraco, Paola; Eriksson, Mats
Journal of Biological Chemistry, Vol. 275, Issue 9
https://doi.org/10.1074/jbc.275.9.6080

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Similar Records

Title: Switchable photosystem-II designer algae for photobiological hydrogen production

Abstract

Citation Formats

Functional Analysis of Photosystem II book,

Temperature effect on production of hydrogen and oxygen byChlamydomonas cold strain CCMP1619 and wild-type 137c: Scientific note journal, September 1995

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans journal, February 1998

Assembly of Photosystem II book,

Killing the messenger: short RNAs that silence gene expression journal, June 2003

Photoinhibition in indica and japonica subspecies of rice (Oryza sativa) and their reciprocal F1 hybrids journal, January 2001

Oxygen sensitivity of algal H2- production journal, March 1997

Improvement of Photosynthetic CO2 Fixation at High Light Intensity Through Reduction of Chlorophyll Antenna Size journal, January 2002

Solar Photobiochemistry: Simultaneous Photoproduction of Hydrogen and Oxygen in a Confined Bioreactor journal, May 2001

Oxygen Tolerance of the H 2 -sensing [NiFe] Hydrogenase from Ralstonia eutropha H16 Is Based on Limited Access of Oxygen to the Active Site journal, April 2005

Cloning of a streptomycin-production gene directing synthesis of N-methyl-l-glucosamine journal, January 1986

A New Oxygen Sensitivity and Its Potential Application in Photosynthetic H2 Production book, January 2003

Functional and Structural Organization of Chlorophyll in the Developing Photosynthetic Membranes of Euglena gracilis Z: II. CHARACTERISTICS OF THE LATE FORMATION OF ACTIVE PHOTOSYSTEM II REACTION CENTERS DURING FIRST STAGES OF GREENING journal, January 1981

Coordinate Copper- and Oxygen-responsive Cyc6 and Cpx1 Expression in Chlamydomonas Is Mediated by the Same Element journal, February 2000

Functional Analysis of Photosystem II
book,

Temperature effect on production of hydrogen and oxygen byChlamydomonas cold strain CCMP1619 and wild-type 137c: Scientific note
journal, September 1995

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
journal, February 1998

Assembly of Photosystem II
book,

Killing the messenger: short RNAs that silence gene expression
journal, June 2003

Photoinhibition in indica and japonica subspecies of rice (Oryza sativa) and their reciprocal F1 hybrids
journal, January 2001

Oxygen sensitivity of algal H2- production
journal, March 1997

Improvement of Photosynthetic CO₂ Fixation at High Light Intensity Through Reduction of Chlorophyll Antenna Size
journal, January 2002

Solar Photobiochemistry: Simultaneous Photoproduction of Hydrogen and Oxygen in a Confined Bioreactor
journal, May 2001

Oxygen Tolerance of the H ₂ -sensing [NiFe] Hydrogenase from Ralstonia eutropha H16 Is Based on Limited Access of Oxygen to the Active Site
journal, April 2005

Cloning of a streptomycin-production gene directing synthesis of N-methyl-l-glucosamine
journal, January 1986

A New Oxygen Sensitivity and Its Potential Application in Photosynthetic H2 Production
book, January 2003

Functional and Structural Organization of Chlorophyll in the Developing Photosynthetic Membranes of Euglena gracilis Z: II. CHARACTERISTICS OF THE LATE FORMATION OF ACTIVE PHOTOSYSTEM II REACTION CENTERS DURING FIRST STAGES OF GREENING
journal, January 1981

Coordinate Copper- and Oxygen-responsive Cyc6 and Cpx1 Expression in Chlamydomonas Is Mediated by the Same Element
journal, February 2000