Insulator-based DEP with impedance measurements for analyte detection

Davalos, Rafael V; Simmons, Blake A; Crocker, Robert W; Cummings, Eric B

Advanced Search OptionsAdvanced Search queries use a traditional Term Search. For more info, see our FAQ.

All Fields:

Patent Title:

Abstract:

Assignee:

Inventor(s):

Patent Number:

Patent Classification (CPC):

All Classifications
A - human necessities
A01 - agriculture
A21 - baking
A22 - butchering
A23 - foods or foodstuffs
A24 - tobacco
A41 - wearing apparel
A42 - headwear
A43 - footwear
A44 - haberdashery
A45 - hand or travelling articles
A46 - brushware
A47 - furniture
A61 - medical or veterinary science
A62 - life-saving
A63 - sports
A99 - subject matter not otherwise provided for in this section
B - performing operations
B01 - physical or chemical processes or apparatus in general
B02 - crushing, pulverising, or disintegrating
B03 - separation of solid materials using liquids or using pneumatic tables or jigs
B04 - centrifugal apparatus or machines for carrying-out physical or chemical processes
B05 - spraying or atomising in general
B06 - generating or transmitting mechanical vibrations in general
B07 - separating solids from solids
B08 - cleaning
B09 - disposal of solid waste
B21 - mechanical metal-working without essentially removing material
B22 - casting
B23 - machine tools
B24 - grinding
B25 - hand tools
B26 - hand cutting tools
B27 - working or preserving wood or similar material
B28 - working cement, clay, or stone
B29 - working of plastics
B30 - presses
B31 - making articles of paper, cardboard or material worked in a manner analogous to paper
B32 - layered products
B33 - additive manufacturing technology
B41 - printing
B42 - bookbinding
B43 - writing or drawing implements
B44 - decorative arts
B60 - vehicles in general
B61 - railways
B62 - land vehicles for travelling otherwise than on rails
B63 - ships or other waterborne vessels
B64 - aircraft
B65 - conveying
B66 - hoisting
B67 - opening, closing {or cleaning} bottles, jars or similar containers
B68 - saddlery
B81 - microstructural technology
B82 - nanotechnology
B99 - subject matter not otherwise provided for in this section
C - chemistry
C01 - inorganic chemistry
C02 - treatment of water, waste water, sewage, or sludge
C03 - glass
C04 - cements
C05 - fertilisers
C06 - explosives
C07 - organic chemistry
C08 - organic macromolecular compounds
C09 - dyes
C10 - petroleum, gas or coke industries
C11 - animal or vegetable oils, fats, fatty substances or waxes
C12 - biochemistry
C13 - sugar industry
C14 - skins
C21 - metallurgy of iron
C22 - metallurgy
C23 - coating metallic material
C25 - electrolytic or electrophoretic processes
C30 - crystal growth
C40 - combinatorial technology
C99 - subject matter not otherwise provided for in this section
D - textiles
D01 - natural or man-made threads or fibres
D02 - yarns
D03 - weaving
D04 - braiding
D05 - sewing
D06 - treatment of textiles or the like
D07 - ropes
D10 - indexing scheme associated with sublasses of section d, relating to textiles
D21 - paper-making
D99 - subject matter not otherwise provided for in this section
E - fixed constructions
E01 - construction of roads, railways, or bridges
E02 - hydraulic engineering
E03 - water supply
E04 - building
E05 - locks
E06 - doors, windows, shutters, or roller blinds in general
E21 - earth drilling
E99 - subject matter not otherwise provided for in this section
F - mechanical engineering
F01 - machines or engines in general
F02 - combustion engines
F03 - machines or engines for liquids
F04 - positive - displacement machines for liquids
F05 - indexing schemes relating to engines or pumps in various subclasses of classes f01-f04
F15 - fluid-pressure actuators
F16 - engineering elements and units
F17 - storing or distributing gases or liquids
F21 - lighting
F22 - steam generation
F23 - combustion apparatus
F24 - heating
F25 - refrigeration or cooling
F26 - drying
F27 - furnaces
F28 - heat exchange in general
F41 - weapons
F42 - ammunition
F99 - subject matter not otherwise provided for in this section
G - physics
G01 - measuring
G02 - optics
G03 - photography
G04 - horology
G05 - controlling
G06 - computing
G07 - checking-devices
G08 - signalling
G09 - education
G10 - musical instruments
G11 - information storage
G12 - instrument details
G16 - information and communication technology [ict] specially adapted for specific application fields
G21 - nuclear physics
G99 - subject matter not otherwise provided for in this section
H - electricity
H01 - basic electric elements
H02 - generation
H03 - basic electronic circuitry
H04 - electric communication technique
H05 - electric techniques not otherwise provided for
H99 - subject matter not otherwise provided for in this section
Y - new / cross sectional technologies
Y02 - technologies or applications for mitigation or adaptation against climate change
Y04 - information or communication technologies having an impact on other technology areas
Y10 - technical subjects covered by former uspc

More Options ...

Title: Insulator-based DEP with impedance measurements for analyte detection

Abstract

Disclosed herein are microfluidic devices for assaying at least one analyte specie in a sample comprising at least one analyte concentration area in a microchannel having insulating structures on or in at least one wall of the microchannel which provide a nonuniform electric field in the presence of an electric field provided by off-chip electrodes; and a pair of passivated sensing electrodes for impedance detection in a detection area. Also disclosed are assay methods and methods of making.

Inventors:

Davalos, Rafael V ^[1]; Simmons, Blake A ^[2]; Crocker, Robert W ^[3]; Cummings, Eric B ^[4]

Blacksburg, VA
San Francisco, CA
Fremont, CA
Livermore, CA

Issue Date:: Tue Mar 16 00:00:00 EDT 2010

Research Org.:: Sandia Corporation (Livermore, CA)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1014491

Patent Number(s):: 7678256

Application Number:: US Patent Application 11/556,685

Assignee:: Sandia Corporation (Livermore, CA)

Patent Classifications (CPCs):: B - PERFORMING OPERATIONS B03 - SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS B03C - MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS

Show more

B - PERFORMING OPERATIONS B03 - SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS B03C - MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS
B03C2201/24 - for measuring or calculating parameters, efficiency, etc.
B03C2201/26 - for use in medical applications
B03C5/005 - {Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
B03C5/026 - {using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]}

Show less

DOE Contract Number:: AC04-94AL85000

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Citation Formats


                    Davalos, Rafael V, Simmons, Blake A, Crocker, Robert W, and Cummings, Eric B. Insulator-based DEP with impedance measurements for analyte detection.  United States: N. p., 2010. 
        Web.

Copy to clipboard


                    Davalos, Rafael V, Simmons, Blake A, Crocker, Robert W, & Cummings, Eric B. Insulator-based DEP with impedance measurements for analyte detection.  United States.

Copy to clipboard


                    Davalos, Rafael V, Simmons, Blake A, Crocker, Robert W, and Cummings, Eric B. Tue .  
        "Insulator-based DEP with impedance measurements for analyte detection".  United States.  https://www.osti.gov/servlets/purl/1014491.

Copy to clipboard


                    
@article{osti_1014491,

  title        = {Insulator-based DEP with impedance measurements for analyte detection},

  author       = {Davalos, Rafael V and Simmons, Blake A and Crocker, Robert W and Cummings, Eric B},

  abstractNote = {Disclosed herein are microfluidic devices for assaying at least one analyte specie in a sample comprising at least one analyte concentration area in a microchannel having insulating structures on or in at least one wall of the microchannel which provide a nonuniform electric field in the presence of an electric field provided by off-chip electrodes; and a pair of passivated sensing electrodes for impedance detection in a detection area. Also disclosed are assay methods and methods of making.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Mar 16 00:00:00 EDT 2010},

  month        = {Tue Mar 16 00:00:00 EDT 2010}

}

Copy to clipboard

Patent:

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Separation of viable and non-viable yeast using dielectrophoresis
journal, January 1994

Markx, Gerard H.; Talary, Mark S.; Pethig, Ronald
Journal of Biotechnology, Vol. 32, Issue 1
https://doi.org/10.1016/0168-1656(94)90117-1

An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water
journal, September 2005

Lapizco-Encinas, Blanca H.; Davalos, Rafael V.; Simmons, Blake A.
Journal of Microbiological Methods, Vol. 62, Issue 3
https://doi.org/10.1016/j.mimet.2005.04.027

Dielectrophoretic Concentration and Separation of Live and Dead Bacteria in an Array of Insulators
journal, March 2004

Lapizco-Encinas, Blanca H.; Simmons, Blake A.; Cummings, Eric B.
Analytical Chemistry, Vol. 76, Issue 6
https://doi.org/10.1021/ac034804j

Dielectrophoresis in Microchips Containing Arrays of Insulating Posts: Theoretical and Experimental Results
journal, September 2003

Cummings, Eric B.; Singh, Anup K.
Analytical Chemistry, Vol. 75, Issue 18
https://doi.org/10.1021/ac0340612

Cell Separation on Microfabricated Electrodes Using Dielectrophoretic/Gravitational Field-Flow Fractionation
journal, March 1999

Yang, Jun; Huang, Ying; Wang, Xiao-Bo
Analytical Chemistry, Vol. 71, Issue 5
https://doi.org/10.1021/ac981250p

Effective Conductivity of a Suspension of Permeabilized Cells: A Theoretical Analysis
journal, August 2003

Pavlin, Mojca; Miklavčič, Damijan
Biophysical Journal, Vol. 85, Issue 2
https://doi.org/10.1016/S0006-3495(03)74515-9

Electrostatic manipulation of DNA in microfabricated structures
journal, January 1990

Washizu, M.; Kurosawa, O.
IEEE Transactions on Industry Applications, Vol. 26, Issue 6
https://doi.org/10.1109/28.62403

High sensitive detection of biological cells using dielectrophoretic impedance measurement method combined with electropermeabilization
journal, November 2003

Suehiro, Junya; Shutou, Masanori; Hatano, Tetsuji
Sensors and Actuators B: Chemical, Vol. 96, Issue 1-2
https://doi.org/10.1016/S0925-4005(03)00517-3

Selective detection of viable bacteria using dielectrophoretic impedance measurement method
journal, February 2003

Suehiro, Junya; Hamada, Ryo; Noutomi, Daisuke
Journal of Electrostatics, Vol. 57, Issue 2
https://doi.org/10.1016/S0304-3886(02)00124-9

Selective detection of specific bacteria using dielectrophoretic impedance measurement method combined with an antigen–antibody reaction
journal, June 2003

Suehiro, Junya; Noutomi, Daisuke; Shutou, Masanori
Journal of Electrostatics, Vol. 58, Issue 3-4
https://doi.org/10.1016/S0304-3886(03)00062-7

Dielectric properties of native and decoated spores of Bacillus megaterium.
journal, January 1979

Carstensen, E. L.; Marquis, R. E.; Child, S. Z.
Journal of Bacteriology, Vol. 140, Issue 3
https://doi.org/10.1128/JB.140.3.917-928.1979

Real-Time Virus Trapping and Fluorescent Imaging in Microfluidic Devices
journal, February 2004

Akin, Demir; Li, Haibo; Bashir, Rashid
Nano Letters, Vol. 4, Issue 2
https://doi.org/10.1021/nl034987p

Electrical impedance-spectroscopy particle detector for use in microanalysis systems
conference, August 1999

Gale, Bruce K.; Frazier, A. Bruno
Symposium on Micromachining and Microfabrication, SPIE Proceedings
https://doi.org/10.1117/12.359337

Electronic manipulation of DNA, proteins, and nanoparticles for potential circuit assembly
journal, October 2004

Zheng, Lifeng; Brody, James P.; Burke, Peter J.
Biosensors and Bioelectronics, Vol. 20, Issue 3
https://doi.org/10.1016/j.bios.2004.03.029

Polymeric microfluidic devices for the monitoring and separation of water-borne pathogens utilizing insulative dielectrophoresis
conference, January 2005

McGraw, Greg J.; Davalos, Rafael V.; Brazzle, John D.
MOEMS-MEMS Micro & Nanofabrication, SPIE Proceedings
https://doi.org/10.1117/12.597711

Insulator-based dielectrophoresis for the selective concentration and separation of live bacteria in water
journal, June 2004

Lapizco-Encinas, Blanca H.; Simmons, Blake A.; Cummings, Eric B.
ELECTROPHORESIS, Vol. 25, Issue 1011
https://doi.org/10.1002/elps.200405899

Similar Records in DOE Patents and OSTI.GOV collections:

Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change

Patent Miles, Robin R [Danville, CA]; Belgrader, Phillip [Severna Park, MD]; Fuller, Christopher D [Oakland, CA]

Impedance measurements are used to detect the end-point for PCR DNA amplification. A pair of spaced electrodes are located on a surface of a microfluidic channel and an AC or DC voltage is applied across the electrodes to produce an electric field. An ionically labeled probe will attach to a complementary DNA segment, and a polymerase enzyme will release the ionic label. This causes the conductivity of the solution in the area of the electrode to change. This change in conductivity is measured as a change in the impedance been the two electrodes.
Full Text Available
Using impedance measurements for detecting pathogens trapped in an electric field

Patent Miles, Robin R.

Impedance measurements between the electrodes in an electric field is utilized to detect the presence of pathogens trapped in the electric field. Since particles trapped in a field using the dielectiphoretic force changes the impedance between the electrodes by changing the dielectric material between the electrodes, the degree of particle trapping can be determined by measuring the impedance. This measurement is used to determine if sufficient pathogen have been collected to analyze further or potentially to identify the pathogen.
Full Text Available
Impedance measurements for detecting pathogens attached to antibodies

Patent Miles, Robin R.; Venkateswaran, Kodumudi S.; Fuller, Christopher K.

The use of impedance measurements to detect the presence of pathogens attached to antibody-coated beads. In a fluidic device antibodies are immobilized on a surface of a patterned interdigitated electrode. Pathogens in a sample fluid streaming past the electrode attach to the immobilized antibodies, which produces a change in impedance between two adjacent electrodes, which impedance change is measured and used to detect the presence of a pathogen. To amplify the signal, beads coated with antibodies are introduced and the beads would stick to the pathogen causing a greater change in impedance between the two adjacent electrodes.
Full Text Available
Apparatus for detecting and recognizing analytes based on their crystallization patterns

Patent Morozov, Victor [Manassas, VA]; Bailey, Charles L [Cross Junction, VA]; Vsevolodov, Nikolai N [Kensington, MD]; ...

The invention contemplates apparatuses for recognition of proteins and other biological molecules by imaging morphology, size and distribution of crystalline and amorphous dry residues in droplets (further referred to as "crystallization patterns") containing predetermined amount of certain crystal-forming organic compounds (reporters) to which protein to be analyzed is added. Changes in the crystallization patterns of a number of amino-acids can be used as a "signature" of a protein added. Also, changes in the crystallization patterns, as well as the character of such changes, can be used as recognition elements in analysis of protein molecules.
Full Text Available
Detection and recognition of analytes based on their crystallization patterns

Patent Morozov, Victor [Manassas, VA]; Bailey, Charles L [Cross Junction, VA]; Vsevolodov, Nikolai N [Kensington, MD]; ...

The invention contemplates a method for recognition of proteins and other biological molecules by imaging morphology, size and distribution of crystalline and amorphous dry residues in droplets (further referred to as "crystallization pattern") containing predetermined amount of certain crystal-forming organic compounds (reporters) to which protein to be analyzed is added. It has been shown that changes in the crystallization patterns of a number of amino-acids can be used as a "signature" of a protein added. It was also found that both the character of changer in the crystallization patter and the fact of such changes can be used as recognitionmore » « less
Full Text Available

Similar Records

Title: Insulator-based DEP with impedance measurements for analyte detection

Abstract

Citation Formats

Separation of viable and non-viable yeast using dielectrophoresis journal, January 1994

An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water journal, September 2005

Dielectrophoretic Concentration and Separation of Live and Dead Bacteria in an Array of Insulators journal, March 2004

Dielectrophoresis in Microchips Containing Arrays of Insulating Posts: Theoretical and Experimental Results journal, September 2003

Cell Separation on Microfabricated Electrodes Using Dielectrophoretic/Gravitational Field-Flow Fractionation journal, March 1999

Effective Conductivity of a Suspension of Permeabilized Cells: A Theoretical Analysis journal, August 2003

Electrostatic manipulation of DNA in microfabricated structures journal, January 1990

High sensitive detection of biological cells using dielectrophoretic impedance measurement method combined with electropermeabilization journal, November 2003

Selective detection of viable bacteria using dielectrophoretic impedance measurement method journal, February 2003

Selective detection of specific bacteria using dielectrophoretic impedance measurement method combined with an antigen–antibody reaction journal, June 2003

Dielectric properties of native and decoated spores of Bacillus megaterium. journal, January 1979

Real-Time Virus Trapping and Fluorescent Imaging in Microfluidic Devices journal, February 2004

Electrical impedance-spectroscopy particle detector for use in microanalysis systems conference, August 1999

Electronic manipulation of DNA, proteins, and nanoparticles for potential circuit assembly journal, October 2004

Polymeric microfluidic devices for the monitoring and separation of water-borne pathogens utilizing insulative dielectrophoresis conference, January 2005

Insulator-based dielectrophoresis for the selective concentration and separation of live bacteria in water journal, June 2004

Separation of viable and non-viable yeast using dielectrophoresis
journal, January 1994

An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water
journal, September 2005

Dielectrophoretic Concentration and Separation of Live and Dead Bacteria in an Array of Insulators
journal, March 2004

Dielectrophoresis in Microchips Containing Arrays of Insulating Posts: Theoretical and Experimental Results
journal, September 2003

Cell Separation on Microfabricated Electrodes Using Dielectrophoretic/Gravitational Field-Flow Fractionation
journal, March 1999

Effective Conductivity of a Suspension of Permeabilized Cells: A Theoretical Analysis
journal, August 2003

Electrostatic manipulation of DNA in microfabricated structures
journal, January 1990

High sensitive detection of biological cells using dielectrophoretic impedance measurement method combined with electropermeabilization
journal, November 2003

Selective detection of viable bacteria using dielectrophoretic impedance measurement method
journal, February 2003

Selective detection of specific bacteria using dielectrophoretic impedance measurement method combined with an antigen–antibody reaction
journal, June 2003

Dielectric properties of native and decoated spores of Bacillus megaterium.
journal, January 1979

Real-Time Virus Trapping and Fluorescent Imaging in Microfluidic Devices
journal, February 2004

Electrical impedance-spectroscopy particle detector for use in microanalysis systems
conference, August 1999

Electronic manipulation of DNA, proteins, and nanoparticles for potential circuit assembly
journal, October 2004

Polymeric microfluidic devices for the monitoring and separation of water-borne pathogens utilizing insulative dielectrophoresis
conference, January 2005

Insulator-based dielectrophoresis for the selective concentration and separation of live bacteria in water
journal, June 2004