Polynucleotides encoding anti-sulfotyrosine antibodies
Abstract
The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.
- Inventors:
-
- Berkeley, CA
- Saint Davids, PA
- Santa Fe, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1013407
- Patent Number(s):
- 7868152
- Application Number:
- 12/560,294
- Assignee:
- Los Alamos National Security, LLC (Los Alamos, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Bertozzi, Carolyn R, Kehoe, John, and Bradbury, Andrew M. Polynucleotides encoding anti-sulfotyrosine antibodies. United States: N. p., 2011.
Web.
Bertozzi, Carolyn R, Kehoe, John, & Bradbury, Andrew M. Polynucleotides encoding anti-sulfotyrosine antibodies. United States.
Bertozzi, Carolyn R, Kehoe, John, and Bradbury, Andrew M. Tue .
"Polynucleotides encoding anti-sulfotyrosine antibodies". United States. https://www.osti.gov/servlets/purl/1013407.
@article{osti_1013407,
title = {Polynucleotides encoding anti-sulfotyrosine antibodies},
author = {Bertozzi, Carolyn R and Kehoe, John and Bradbury, Andrew M},
abstractNote = {The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 11 00:00:00 EST 2011},
month = {Tue Jan 11 00:00:00 EST 2011}
}
Works referenced in this record:
Detection and Purification of Tyrosine-sulfated Proteins Using a Novel Anti-sulfotyrosine Monoclonal Antibody
journal, October 2006
- Hoffhines, Adam J.; Damoc, Eugen; Bridges, Kristie G.
- Journal of Biological Chemistry, Vol. 281, Issue 49