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Title: Anti-sulfotyrosine antibodies

Abstract

The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.

Inventors:
 [1];  [2];  [3]
  1. Berkeley, CA
  2. Saint Davids, PA
  3. Santa Fe, NM
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
988814
Patent Number(s):
7589182
Application Number:
11/900,689
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Bertozzi, Carolyn R, Kehoe, John, and Bradbury, Andrew M. Anti-sulfotyrosine antibodies. United States: N. p., 2009. Web.
Bertozzi, Carolyn R, Kehoe, John, & Bradbury, Andrew M. Anti-sulfotyrosine antibodies. United States.
Bertozzi, Carolyn R, Kehoe, John, and Bradbury, Andrew M. Tue . "Anti-sulfotyrosine antibodies". United States. https://www.osti.gov/servlets/purl/988814.
@article{osti_988814,
title = {Anti-sulfotyrosine antibodies},
author = {Bertozzi, Carolyn R and Kehoe, John and Bradbury, Andrew M},
abstractNote = {The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {9}
}

Works referenced in this record:

beta-Endorphin omission analogs: dissociation of immunoreactivity from other biological activities.
journal, June 1980


Single amino acid substitution altering antigen-binding specificity.
journal, March 1982


Exploiting recombination in single bacteria to make large phage antibody libraries
journal, January 2000


Tyrosine Sulfation and the Secretory Pathway
journal, October 1988


A single amino acid substitution in a common african allele of the CD4 molecule ablates binding of the monoclonal antibody, OKT4
journal, November 1991