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Title: Biosensors based on directed assembly of particles

Abstract

A sensor system for detecting an effector or cofactor comprises (a) a nucleic acid enzyme; (b) a substrate for the nucleic acid enzyme, comprising a first polynucleotide; (c) a first set of particles comprising a second polynucleotide at least partially complementary to the substrate, where the polynucleotide is attached to the particles at its 3' terminus; and (d) a second set of particles comprising a third polynucleotide at least partially complementary to the substrate, where the polynucleotide is attached to the particles at its 5' terminus.

Inventors:
 [1];  [2]
  1. Champaign, IL
  2. Urbana, IL
Issue Date:
Research Org.:
Univ. of Illinois at Urbana-Champaign, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
988364
Patent Number(s):
7485419
Application Number:
10/756,825
Assignee:
The Board of Trustees of the University of Illinois (Urbana, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-01ER63179
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lu, Yi, and Liu, Juewen. Biosensors based on directed assembly of particles. United States: N. p., 2009. Web.
Lu, Yi, & Liu, Juewen. Biosensors based on directed assembly of particles. United States.
Lu, Yi, and Liu, Juewen. Tue . "Biosensors based on directed assembly of particles". United States. https://www.osti.gov/servlets/purl/988364.
@article{osti_988364,
title = {Biosensors based on directed assembly of particles},
author = {Lu, Yi and Liu, Juewen},
abstractNote = {A sensor system for detecting an effector or cofactor comprises (a) a nucleic acid enzyme; (b) a substrate for the nucleic acid enzyme, comprising a first polynucleotide; (c) a first set of particles comprising a second polynucleotide at least partially complementary to the substrate, where the polynucleotide is attached to the particles at its 3' terminus; and (d) a second set of particles comprising a third polynucleotide at least partially complementary to the substrate, where the polynucleotide is attached to the particles at its 5' terminus.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {2}
}

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In vitro selection of self-cleaving DNAs
journal, December 1996


Selection and design of high-affinity rna ligands for hiv-1 rev
journal, December 1993


Molecular Aptamer Beacons for Real-Time Protein Recognition
journal, March 2002


Photodynamic Fluorescent Metal Ion Sensors with Parts per Billion Sensitivity
journal, April 1998


Label-Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer-Based Sensor
journal, August 2005


Crystal structure of a four-stranded intercalated DNA: d(C4)
journal, November 1994


Viral-Induced Self-Assembly of Magnetic Nanoparticles Allows the Detection of Viral Particles in Biological Media
journal, August 2003

  • Perez, J. Manuel; Simeone, F. Joseph; Saeki, Yoshinaga
  • Journal of the American Chemical Society, Vol. 125, Issue 34, p. 10192-10193
  • https://doi.org/10.1021/ja036409g

Imaging of the intracellular topography of copper with a fluorescent sensor and by synchrotron x-ray fluorescence microscopy
journal, August 2005


Programmed Assembly of DNA Functionalized Quantum Dots
journal, September 1999


Synthesis from DNA of a molecule with the connectivity of a cube
journal, April 1991


Engineering precision RNA molecular switches
journal, March 1999


Gadolinium-DTPA as a contrast agent in MRI: initial clinical experience in 20 patients
journal, August 1984


A Colorimetric Lead Biosensor Using DNAzyme-Directed Assembly of Gold Nanoparticles
journal, June 2003


DNA aptamers and DNA enzymes
journal, June 1997


Redox Switchable Fluorescent Probe Selective for Either Hg(II) or Cd(II) and Zn(II)
journal, June 1999


What Controls the Melting Properties of DNA-Linked Gold Nanoparticle Assemblies?
journal, February 2003


A specific quadrilateral synthesized from DNA branched junctions
journal, August 1989


In vitro selection of hematoporphyrin binding DNA aptamers
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A catalytic beacon sensor for uranium with parts-per-trillion sensitivity and millionfold selectivity
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In vitro selection for altered divalent metal specificity in the RNase P RNA
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Emerging concepts in molecular MRI
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A Selective and Sensitive Fluoroionophore for Hg II , Ag I , and Cu II with Virtually Decoupled Fluorophore and Receptor Units
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Optical sensor for the visual detection of mercury using mesoporous silica anchoring porphyrin moiety
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Uranyl Acetate Causes DNA Single Strand Breaks In Vitro in the Presence of Ascorbate (Vitamin C)
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Targeting of ultrasmall superparamagnetic iron oxide (USPIO) particles to tumor cellsin Vivo by using transferrin receptor pathways
journal, August 1998


The risk of lead toxicity in homes with lead paint hazard
journal, February 1991


Determination of the orientation of aromatic molecules adsorbed on platinum electrodes: the influence of iodide, a surface-active anion
journal, May 1982


Catalytic Signal Amplification Using a Heck Reaction. An Example in the Fluorescence Sensing of Cu(II)
journal, November 2004


Adaptive Recognition by Nucleic Acid Aptamers
journal, February 2000


Preparation and Characterization of Au Colloid Monolayers
journal, February 1995


Detection of small organic analytes by fluorescing molecular switches
journal, October 2001


DNA in a material world
journal, January 2003


A Calcium-Sensitive Magnetic Resonance Imaging Contrast Agent
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A Highly Selective and Sensitive Fluorescent Chemosensor for Hg 2+ in Neutral Buffer Aqueous Solution
journal, March 2004


Phosphorylating DNA with DNA
journal, March 1999


Directed Evolution of Nucleic Acid Enzymes
journal, June 2004


Optical fibre reflectance sensors for the detection of heavy metal ions based on immobilised Br-PADAP
journal, August 1998


The effects of iron oxides on proton relaxivity
journal, November 1988


An Autonomous DNA Nanomotor Powered by a DNA Enzyme
journal, July 2004


Alloy Formation of Gold−Silver Nanoparticles and the Dependence of the Plasmon Absorption on Their Composition
journal, May 1999


Immobilized RNA switches for the analysis of complex chemical and biological mixtures
journal, April 2001


Engineering DNA aptamers and DNA enzymes with fluorescence-signaling properties
journal, January 2004


Noninvasive Detection of Clinically Occult Lymph-Node Metastases in Prostate Cancer
journal, June 2003


DNA ligands that bind tightly and selectively to cellobiose
journal, May 1998


Aptamer-Based Colorimetric Probe for Cocaine
journal, August 2002


Fluorescent peptide sensor for the selective detection of Cu2+
journal, March 2007


Self-Assembled Monolayers of Thiol-End-Labeled DNA at Mercury Electrodes
journal, July 2006


A two-channel molecular dosimeter for the optical detection of copper( ii )
journal, January 2003


Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures
journal, February 1992


Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena
journal, November 1982


Oligonucleotide immobilization on micropatterned streptavidin surfaces
journal, April 2000


The influence of the blood levels of lead, aluminum and vanadium upon the arterial hypertension
journal, January 1995


Light-directed, spatially addressable parallel chemical synthesis
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Determination of Lead in Blood by Square Wave Anodic Stripping Voltammetry at a Carbon Disk Ultramicroelectrode
journal, July 1994


Two Decades of RNA Catalysis
journal, September 2002


A DNA metalloenzyme with DNA ligase activity
journal, June 1995


Peptide platforms for metal ion sensing
conference, December 1999


RNA-catalysed RNA polymerization using nucleoside triphosphates
journal, July 1996


A Reagentless Signal-On Architecture for Electronic, Aptamer-Based Sensors via Target-Induced Strand Displacement
journal, December 2005


A Selective, Ratiometric Fluorescent Sensor for Pb 2+
journal, January 2000


Defining a Smaller RNA Substrate for Elongation Factor Tu
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The Chemical Form of Mercury in Fish
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Assembly and characterization of five-arm and six-arm DNA branched junctions
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DNA Aptamers Selected Against the HIV-1 trans -Activation-responsive RNA Element Form RNA-DNA Kissing Complexes
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Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics
journal, September 1999


Rapid identification and characterization of hammerhead-ribozyme inhibitors using fluorescence-based technology
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Evidence for the metal-cofactor independence of an RNA phosphodiester-cleaving DNA enzyme
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Dinucleotide Junction Cleavage Versatility of 8-17 Deoxyribozyme
journal, January 2004


Photoactive chemosensors 3 : a unique case of fluorescence enhancement with Cu( ii )
journal, January 2002


Capillary Optode: Determination of Mercury(II) in Aqueous Solution
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Peptide bond formation by in vitro selected ribozymes
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Ribozyme-catalysed amino-acid transfer reactions
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In Vitro Selection of Functional Nucleic Acids
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Programmed Materials Synthesis with DNA
journal, July 1999


A Lead-Dependent DNAzyme with a Two-Step Mechanism
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Activation of Sphingosine Kinase by Tumor Necrosis Factor-α Inhibits Apoptosis in Human Endothelial Cells
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DNA-Modified Core−Shell Ag/Au Nanoparticles
journal, August 2001


Graphite furnace atomic absorption spectroscopic measurement of blood lead in matrix-matched standards
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Antibody-based sensors for heavy metal ions
journal, December 2001


Aptamer-Based Folding Fluorescent Sensor for Cocaine
journal, May 2001


A two-metal ion mechanism operates in the hammerhead ribozyme-mediated cleavage of an RNA substrate
journal, January 1998


Structural diversity of self-cleaving ribozymes
journal, May 2000


Deoxyribozymes: New players in the ancient game of biocatalysis
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Fluorescent Sensors for Zn 2+ Based on a Fluorescein Platform:  Synthesis, Properties and Intracellular Distribution
journal, August 2001


Magnetic Sensors for Protease Assays
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Highly Selective and Sensitive Phosphane Sulfide Derivative for the Detection of Hg 2+ in an Organoaqueous Medium
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A Combinatorial Approach To Discover New Chelators for Optical Metal Ion Sensing
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Molecular Cloning and Functional Characterization of Murine Sphingosine Kinase
journal, September 1998


A New Fluorescent Chemosensor for Copper Ions Based on Tripeptide Glycyl−Histidyl−Lysine (GHK)
journal, October 2001


A novel RNA motif for neomycin recognition
journal, August 1995


Interference-based detection of nucleic acid targets on optically coated silicon
journal, January 2001


Programmable self-assembly of metal ions inside artificial DNA duplexes
journal, November 2006


In vitro selection of RNA aptamers specific for cyanocobalamin
journal, February 1994


Thrombin-binding DNA aptamer forms a unimolecular quadruplex structure in solution.
journal, April 1993


Expanding the scope of RNA catalysis
journal, June 1994


DNA aptamers that bind to chitin
journal, March 2000


Colorimetric Cu2+ detection with a ligation DNAzyme and nanoparticles
journal, January 2007


Mercury Detection in Seawater Using a Mercaptoacetic Acid Modified Gold Microwire Electrode
journal, May 2005


Determination of Picomolar Concentrations of Metal Ions Using Fluorescence Anisotropy:  Biosensing with a “Reagentless” Enzyme Transducer
journal, November 1998


A colorimetric sensor array for odour visualization
journal, August 2000


Label-Free Electrochemical Detection for Aptamer-Based Array Electrodes
journal, August 2005