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Title: Repressor-mediated tissue-specific gene expression in plants

Abstract

Plant tissue specific gene expression by way of repressor-operator complexes, has enabled outcomes including, without limitation, male sterility and engineered plants having root-specific gene expression of relevant proteins to clean environmental pollutants from soil and water. A mercury hyperaccumulation strategy requires that mercuric ion reductase coding sequence is strongly expressed. The actin promoter vector, A2pot, engineered to contain bacterial lac operator sequences, directed strong expression in all plant vegetative organs and tissues. In contrast, the expression from the A2pot construct was restricted primarily to root tissues when a modified bacterial repressor (LacIn) was coexpressed from the light-regulated rubisco small subunit promoter in above-ground tissues. Also provided are analogous repressor operator complexes for selective expression in other plant tissues, for example, to produce male sterile plants.

Inventors:
 [1];  [2];  [1];  [1]
  1. Athens, GA
  2. Oxford, OH
Issue Date:
Research Org.:
University of Georgia Research Foundation, Inc. (Athens, GA)
Sponsoring Org.:
USDOE
OSTI Identifier:
988296
Patent Number(s):
7491811
Application Number:
11/038,900
Assignee:
University of Georgia Research Foundation, Inc. (Athens, GA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG07-02ER63493
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Meagher, Richard B, Balish, Rebecca S, Tehryung, Kim, and McKinney, Elizabeth C. Repressor-mediated tissue-specific gene expression in plants. United States: N. p., 2009. Web.
Meagher, Richard B, Balish, Rebecca S, Tehryung, Kim, & McKinney, Elizabeth C. Repressor-mediated tissue-specific gene expression in plants. United States.
Meagher, Richard B, Balish, Rebecca S, Tehryung, Kim, and McKinney, Elizabeth C. Tue . "Repressor-mediated tissue-specific gene expression in plants". United States. https://www.osti.gov/servlets/purl/988296.
@article{osti_988296,
title = {Repressor-mediated tissue-specific gene expression in plants},
author = {Meagher, Richard B and Balish, Rebecca S and Tehryung, Kim and McKinney, Elizabeth C},
abstractNote = {Plant tissue specific gene expression by way of repressor-operator complexes, has enabled outcomes including, without limitation, male sterility and engineered plants having root-specific gene expression of relevant proteins to clean environmental pollutants from soil and water. A mercury hyperaccumulation strategy requires that mercuric ion reductase coding sequence is strongly expressed. The actin promoter vector, A2pot, engineered to contain bacterial lac operator sequences, directed strong expression in all plant vegetative organs and tissues. In contrast, the expression from the A2pot construct was restricted primarily to root tissues when a modified bacterial repressor (LacIn) was coexpressed from the light-regulated rubisco small subunit promoter in above-ground tissues. Also provided are analogous repressor operator complexes for selective expression in other plant tissues, for example, to produce male sterile plants.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {2}
}

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