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Title: Methods and materials for the production of L-lactic acid in yeast

Abstract

Recombinant yeast are provided having, in one aspect, multiple exogenous LDH genes integrated into the genome, while leaving native PDC genes intact. In a second aspect, recombinant yeast are provided having an exogenous LDH gene integrated into its genome at the locus of a native PDC gene, with deletion of the native PDC gene. The recombinant yeast are useful in fermentation process for producing lactic acid.

Inventors:
 [1];  [2];  [3]
  1. Jordan, MN
  2. Minnetonka, MN
  3. Maple Grove, MN
Issue Date:
Research Org.:
Cargill, Inc. (Wayzata, MN)
Sponsoring Org.:
USDOE
OSTI Identifier:
988145
Patent Number(s):
7534597
Application Number:
10/452,038
Assignee:
Cargill, Inc. (Wayzata, MN)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FC36-00GO10598
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Hause, Ben, Rajgarhia, Vineet, and Suominen, Pirkko. Methods and materials for the production of L-lactic acid in yeast. United States: N. p., 2009. Web.
Hause, Ben, Rajgarhia, Vineet, & Suominen, Pirkko. Methods and materials for the production of L-lactic acid in yeast. United States.
Hause, Ben, Rajgarhia, Vineet, and Suominen, Pirkko. Tue . "Methods and materials for the production of L-lactic acid in yeast". United States. https://www.osti.gov/servlets/purl/988145.
@article{osti_988145,
title = {Methods and materials for the production of L-lactic acid in yeast},
author = {Hause, Ben and Rajgarhia, Vineet and Suominen, Pirkko},
abstractNote = {Recombinant yeast are provided having, in one aspect, multiple exogenous LDH genes integrated into the genome, while leaving native PDC genes intact. In a second aspect, recombinant yeast are provided having an exogenous LDH gene integrated into its genome at the locus of a native PDC gene, with deletion of the native PDC gene. The recombinant yeast are useful in fermentation process for producing lactic acid.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {5}
}

Works referenced in this record:

Identification of a gene encoding the pyruvate decarboxylase gene regulator CaPdc2p fromCandida albicans
journal, May 1999


Cloning, characterization and insertional inactivation of the Lactobacillus helveticus D(?) lactate dehydrogenase gene
journal, June 1994


Development of metabolically engineered Saccharomyces cerevisiae cells for the production of lactic acid
journal, May 1995


A deletion of the PDC1 gene for pyruvate decarboxylase of yeast causes a different phenotype than previously isolated point mutations
journal, February 1989


Genetic manipulation of the pathogenic yeast Candida parapsilosis
journal, October 2002


Identification, cloning and characterisation of a new gene required for full pyruvate decarboxylase activity in Saccharomyces cerevisiae
journal, March 1989


The isolation and nucleotide sequence of the pyruvate decarboxylase gene from Kluyveromyces marxianus
journal, September 1993