Apparatus and method for tracking a molecule or particle in three dimensions

Werner, James H; Goodwin, Peter M; Lessard, Guillaume

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Title: Apparatus and method for tracking a molecule or particle in three dimensions

Abstract

An apparatus and method were used to track the movement of fluorescent particles in three dimensions. Control software was used with the apparatus to implement a tracking algorithm for tracking the motion of the individual particles in glycerol/water mixtures. Monte Carlo simulations suggest that the tracking algorithms in combination with the apparatus may be used for tracking the motion of single fluorescent or fluorescently labeled biomolecules in three dimensions.

Inventors:

Werner, James H ^[1]; Goodwin, Peter M ^[1]; Lessard, Guillaume ^[2]

Los Alamos, NM
Santa Fe, NM

Issue Date:: 2009-03-03

Research Org.:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 986482

Patent Number(s):: 7498551

Application Number:: 11/544,988

Assignee:: Los Alamos National Security, LLC (Los Alamos, NM)

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G - PHYSICS G02 - OPTICS G02B - OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS

Show more

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N15/1475 - {using image analysis for extracting features of the particle}
G01N2015/1075 - {Determining speed or velocity of a particle}
G01N2021/1785 - {Three dimensional}
G01N2021/6471 - {Special filters, filter wheel}
G01N21/4795 - {spatially resolved investigating of object in scattering medium
G01N21/6408 - {with measurement of decay time, time resolved fluorescence}
G01N21/6428 - {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
G01N21/6458 - {Fluorescence microscopy

G - PHYSICS G02 - OPTICS G02B - OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
G02B21/0076 - {arrangements using fluorescence or luminescence}

Show less

DOE Contract Number:: AC52-06NA25396

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 47 OTHER INSTRUMENTATION

Citation Formats


                    Werner, James H, Goodwin, Peter M, and Lessard, Guillaume. Apparatus and method for tracking a molecule or particle in three dimensions.  United States: N. p., 2009. 
        Web.

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                    Werner, James H, Goodwin, Peter M, & Lessard, Guillaume. Apparatus and method for tracking a molecule or particle in three dimensions.  United States.

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                    Werner, James H, Goodwin, Peter M, and Lessard, Guillaume. Tue .  
        "Apparatus and method for tracking a molecule or particle in three dimensions".  United States.  https://www.osti.gov/servlets/purl/986482.

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@article{osti_986482,

  title        = {Apparatus and method for tracking a molecule or particle in three dimensions},

  author       = {Werner, James H and Goodwin, Peter M and Lessard, Guillaume},

  abstractNote = {An apparatus and method were used to track the movement of fluorescent particles in three dimensions. Control software was used with the apparatus to implement a tracking algorithm for tracking the motion of the individual particles in glycerol/water mixtures. Monte Carlo simulations suggest that the tracking algorithms in combination with the apparatus may be used for tracking the motion of single fluorescent or fluorescently labeled biomolecules in three dimensions.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2009},

  month        = {3}

}

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Works referenced in this record:

Variable Incidence Angle Fluorescence Interference Contrast Microscopy for Z-Imaging Single Objects
journal, October 2005

Ajo-Franklin, Caroline M.; Ganesan, Prasad V.; Boxer, Steven G.
Biophysical Journal, Vol. 89, Issue 4
https://doi.org/10.1529/biophysj.105.066738

Three-dimensional fluorescent particle tracking at micron-scale using a single camera
journal, February 2005

Wu, Mingming; Roberts, John W.; Buckley, Mark
Experiments in Fluids, Vol. 38, Issue 4
https://doi.org/10.1007/s00348-004-0925-9

Positional and Temporal Accuracy of Single Molecule Tracking
journal, September 2000

Enderlein, Jörg
Single Molecules, Vol. 1, Issue 3, p. 225-230
URL: 10.1002/1438-5171(200009)1:3<225::AID-SIMO225>3.0.CO;2-O

[38] Versatile optical traps with feedback control
book, January 1998

Visscher, Koen; Block, Steven M.
Molecular Motors and the Cytoskeleton Part B
https://doi.org/10.1016/S0076-6879(98)98040-5

Tracking of fluorescent molecules diffusing within membranes
journal, November 2000

Enderlein, J.
Applied Physics B, Vol. 71, Issue 5
https://doi.org/10.1007/s003400000409

Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics
journal, January 2005

Michalet, X.
Science, Vol. 307, Issue 5709
https://doi.org/10.1126/science.1104274

Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon
journal, October 1997

Braun, D.; Fromherz, P.
Applied Physics A: Materials Science & Processing, Vol. 65, Issue 4-5
https://doi.org/10.1007/s003390050589

Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position
journal, September 1994

Kao, H. P.; Verkman, A. S.
Biophysical Journal, Vol. 67, Issue 3, p. 1291-1300
https://doi.org/10.1016/S0006-3495(94)80601-0

SINGLE-PARTICLE TRACKING:Applications to Membrane Dynamics
journal, June 1997

Saxton, Michael J.; Jacobson, Ken
Annual Review of Biophysics and Biomolecular Structure, Vol. 26, Issue 1
https://doi.org/10.1146/annurev.biophys.26.1.373

Simultaneous Imaging of Different Focal Planes in Fluorescence Microscopy for the Study of Cellular Dynamics in Three Dimensions
journal, December 2004

Prabhat, P.; Ram, S.; Ward, E. S.
IEEE Transactions on Nanobioscience, Vol. 3, Issue 4
https://doi.org/10.1109/TNB.2004.837899

Tracking-FCS: Fluorescence correlation spectroscopy of individual particles
journal, January 2005

Berglund, Andrew J.; Mabuchi, Hideo
Optics Express, Vol. 13, Issue 20
https://doi.org/10.1364/OPEX.13.008069

Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels
journal, November 1996

Dickson, Robert M.; Norris, D. J.; Tzeng, Yih-Ling
Science, Vol. 274, Issue 5289
https://doi.org/10.1126/science.274.5289.966

3-D Particle Tracking in a Two-Photon Microscope: Application to the Study of Molecular Dynamics in Cells
journal, April 2005

Levi, Valeria; Ruan, QiaoQiao; Gratton, Enrico
Biophysical Journal, Vol. 88, Issue 4
https://doi.org/10.1529/biophysj.104.044230

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Similar Records

Title: Apparatus and method for tracking a molecule or particle in three dimensions

Abstract

Citation Formats

Variable Incidence Angle Fluorescence Interference Contrast Microscopy for Z-Imaging Single Objects journal, October 2005

Three-dimensional fluorescent particle tracking at micron-scale using a single camera journal, February 2005

Positional and Temporal Accuracy of Single Molecule Tracking journal, September 2000

[38] Versatile optical traps with feedback control book, January 1998

Tracking of fluorescent molecules diffusing within membranes journal, November 2000

Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics journal, January 2005

Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon journal, October 1997

Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position journal, September 1994

SINGLE-PARTICLE TRACKING:Applications to Membrane Dynamics journal, June 1997

Simultaneous Imaging of Different Focal Planes in Fluorescence Microscopy for the Study of Cellular Dynamics in Three Dimensions journal, December 2004

Tracking-FCS: Fluorescence correlation spectroscopy of individual particles journal, January 2005

Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels journal, November 1996

3-D Particle Tracking in a Two-Photon Microscope: Application to the Study of Molecular Dynamics in Cells journal, April 2005

Variable Incidence Angle Fluorescence Interference Contrast Microscopy for Z-Imaging Single Objects
journal, October 2005

Three-dimensional fluorescent particle tracking at micron-scale using a single camera
journal, February 2005

Positional and Temporal Accuracy of Single Molecule Tracking
journal, September 2000

[38] Versatile optical traps with feedback control
book, January 1998

Tracking of fluorescent molecules diffusing within membranes
journal, November 2000

Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics
journal, January 2005

Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon
journal, October 1997

Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position
journal, September 1994

SINGLE-PARTICLE TRACKING:Applications to Membrane Dynamics
journal, June 1997

Simultaneous Imaging of Different Focal Planes in Fluorescence Microscopy for the Study of Cellular Dynamics in Three Dimensions
journal, December 2004

Tracking-FCS: Fluorescence correlation spectroscopy of individual particles
journal, January 2005

Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels
journal, November 1996

3-D Particle Tracking in a Two-Photon Microscope: Application to the Study of Molecular Dynamics in Cells
journal, April 2005