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Title: Apparatus and method for tracking a molecule or particle in three dimensions

Abstract

An apparatus and method were used to track the movement of fluorescent particles in three dimensions. Control software was used with the apparatus to implement a tracking algorithm for tracking the motion of the individual particles in glycerol/water mixtures. Monte Carlo simulations suggest that the tracking algorithms in combination with the apparatus may be used for tracking the motion of single fluorescent or fluorescently labeled biomolecules in three dimensions.

Inventors:
 [1];  [1];  [2]
  1. Los Alamos, NM
  2. Santa Fe, NM
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
986482
Patent Number(s):
7498551
Application Number:
11/544,988
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G - PHYSICS G02 - OPTICS G02B - OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
DOE Contract Number:  
AC52-06NA25396
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Werner, James H, Goodwin, Peter M, and Lessard, Guillaume. Apparatus and method for tracking a molecule or particle in three dimensions. United States: N. p., 2009. Web.
Werner, James H, Goodwin, Peter M, & Lessard, Guillaume. Apparatus and method for tracking a molecule or particle in three dimensions. United States.
Werner, James H, Goodwin, Peter M, and Lessard, Guillaume. Tue . "Apparatus and method for tracking a molecule or particle in three dimensions". United States. https://www.osti.gov/servlets/purl/986482.
@article{osti_986482,
title = {Apparatus and method for tracking a molecule or particle in three dimensions},
author = {Werner, James H and Goodwin, Peter M and Lessard, Guillaume},
abstractNote = {An apparatus and method were used to track the movement of fluorescent particles in three dimensions. Control software was used with the apparatus to implement a tracking algorithm for tracking the motion of the individual particles in glycerol/water mixtures. Monte Carlo simulations suggest that the tracking algorithms in combination with the apparatus may be used for tracking the motion of single fluorescent or fluorescently labeled biomolecules in three dimensions.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {3}
}

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Works referenced in this record:

Variable Incidence Angle Fluorescence Interference Contrast Microscopy for Z-Imaging Single Objects
journal, October 2005


Three-dimensional fluorescent particle tracking at micron-scale using a single camera
journal, February 2005


Tracking of fluorescent molecules diffusing within membranes
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Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics
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Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon
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Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position
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SINGLE-PARTICLE TRACKING:Applications to Membrane Dynamics
journal, June 1997


Simultaneous Imaging of Different Focal Planes in Fluorescence Microscopy for the Study of Cellular Dynamics in Three Dimensions
journal, December 2004


Tracking-FCS: Fluorescence correlation spectroscopy of individual particles
journal, January 2005


Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels
journal, November 1996


3-D Particle Tracking in a Two-Photon Microscope: Application to the Study of Molecular Dynamics in Cells
journal, April 2005