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Title: Oxygen-resistant hydrogenases and methods for designing and making same

Abstract

The invention provides oxygen- resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.

Inventors:
 [1];  [2];  [2]
  1. Golden, CO
  2. Lakewood, CO
Issue Date:
Research Org.:
Midwest Research Institute, Kansas City, MO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
986476
Patent Number(s):
7501270
Application Number:
10/553,097
Assignee:
Allaince for Sustainable Energy, LLC (Golden, CO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC36-99GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

King, Paul, Ghirardi, Maria L, and Seibert, Michael. Oxygen-resistant hydrogenases and methods for designing and making same. United States: N. p., 2009. Web.
King, Paul, Ghirardi, Maria L, & Seibert, Michael. Oxygen-resistant hydrogenases and methods for designing and making same. United States.
King, Paul, Ghirardi, Maria L, and Seibert, Michael. Tue . "Oxygen-resistant hydrogenases and methods for designing and making same". United States. https://www.osti.gov/servlets/purl/986476.
@article{osti_986476,
title = {Oxygen-resistant hydrogenases and methods for designing and making same},
author = {King, Paul and Ghirardi, Maria L and Seibert, Michael},
abstractNote = {The invention provides oxygen- resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {3}
}

Patent:

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