DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Hybrid magnet devices for molecule manipulation and small scale high gradient-field applications

Abstract

The present disclosure provides a high performance hybrid magnetic structure made from a combination of permanent magnets and ferromagnetic pole materials which are assembled in a predetermined array. The hybrid magnetic structure provides means for separation and other biotechnology applications involving holding, manipulation, or separation of magnetizable molecular structures and targets. Also disclosed are hybrid magnetic tweezers able to exert approximately 1 nN of force to 4.5 .mu.m magnetic bead. The maximum force was experimentally measured to be .about.900 pN which is in good agreement with theoretical estimations and other measurements. In addition, a new analysis scheme that permits fast real-time position measurement in typical geometry of magnetic tweezers has been developed and described in detail.

Inventors:
 [1];  [2];  [3];  [1];  [4]
  1. El Cerrito, CA
  2. Seoul, KR
  3. (Berkeley, CA)
  4. Berkeley, CA
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
985856
Patent Number(s):
7474184
Application Number:
11/355,462
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B03 - SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS B03C - MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS
H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01F - MAGNETS
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Humphries, David E, Hong, Seok-Cheol, Cozzarelli, legal representative, Linda A., Pollard, Martin J, and Cozzarelli, Nicholas R. Hybrid magnet devices for molecule manipulation and small scale high gradient-field applications. United States: N. p., 2009. Web.
Humphries, David E, Hong, Seok-Cheol, Cozzarelli, legal representative, Linda A., Pollard, Martin J, & Cozzarelli, Nicholas R. Hybrid magnet devices for molecule manipulation and small scale high gradient-field applications. United States.
Humphries, David E, Hong, Seok-Cheol, Cozzarelli, legal representative, Linda A., Pollard, Martin J, and Cozzarelli, Nicholas R. Tue . "Hybrid magnet devices for molecule manipulation and small scale high gradient-field applications". United States. https://www.osti.gov/servlets/purl/985856.
@article{osti_985856,
title = {Hybrid magnet devices for molecule manipulation and small scale high gradient-field applications},
author = {Humphries, David E and Hong, Seok-Cheol and Cozzarelli, legal representative, Linda A. and Pollard, Martin J and Cozzarelli, Nicholas R},
abstractNote = {The present disclosure provides a high performance hybrid magnetic structure made from a combination of permanent magnets and ferromagnetic pole materials which are assembled in a predetermined array. The hybrid magnetic structure provides means for separation and other biotechnology applications involving holding, manipulation, or separation of magnetizable molecular structures and targets. Also disclosed are hybrid magnetic tweezers able to exert approximately 1 nN of force to 4.5 .mu.m magnetic bead. The maximum force was experimentally measured to be .about.900 pN which is in good agreement with theoretical estimations and other measurements. In addition, a new analysis scheme that permits fast real-time position measurement in typical geometry of magnetic tweezers has been developed and described in detail.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {1}
}

Works referenced in this record:

Near-field-magnetic-tweezer manipulation of single DNA molecules
journal, July 2004


Magnetic tweezers for DNA micromanipulation
journal, January 2000


Magnetic tweezers for intracellular applications
journal, September 2003


Magnetic tweezers: a sensitive tool to study DNA and chromatin at the single-molecule level
journal, June 2003


Magnetic Tweezers: Micromanipulation and Force Measurement at the Molecular Level
journal, June 2002


The Elasticity of a Single Supercoiled DNA Molecule
journal, March 1996