Peptides whose uptake by cells is controllable
Abstract
A generic structure for the peptides of the present invention includes A-X-B-C, where C is a cargo moiety, the B portion includes basic amino acids, X is a cleavable linker sequence, and the A portion includes acidic amino acids. The intact structure is not significantly taken up by cells; however, upon extracellular cleavage of X, the B-C portion is taken up, delivering the cargo to targeted cells. Cargo may be, for example, a contrast agent for diagnostic imaging, a chemotherapeutic drug, or a radiation-sensitizer for therapy. Cleavage of X allows separation of A from B, unmasking the normal ability of the basic amino acids in B to drag cargo C into cells near the cleavage event. X is cleaved extracellularly, preferably under physiological conditions. D-amino acids are preferred for the A and B portions, to minimize immunogenicity and nonspecific cleavage by background peptidases or proteases.
- Inventors:
-
- San Diego, CA
- La Jolla, CA
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 985413
- Patent Number(s):
- 7431915
- Application Number:
- 10/699,562
- Assignee:
- The Regents of the University of California (La Jolla, CA)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
- DOE Contract Number:
- FG03-01ER63276
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Jiang, Tao, and Tsien, Roger Y. Peptides whose uptake by cells is controllable. United States: N. p., 2008.
Web.
Jiang, Tao, & Tsien, Roger Y. Peptides whose uptake by cells is controllable. United States.
Jiang, Tao, and Tsien, Roger Y. Tue .
"Peptides whose uptake by cells is controllable". United States. https://www.osti.gov/servlets/purl/985413.
@article{osti_985413,
title = {Peptides whose uptake by cells is controllable},
author = {Jiang, Tao and Tsien, Roger Y},
abstractNote = {A generic structure for the peptides of the present invention includes A-X-B-C, where C is a cargo moiety, the B portion includes basic amino acids, X is a cleavable linker sequence, and the A portion includes acidic amino acids. The intact structure is not significantly taken up by cells; however, upon extracellular cleavage of X, the B-C portion is taken up, delivering the cargo to targeted cells. Cargo may be, for example, a contrast agent for diagnostic imaging, a chemotherapeutic drug, or a radiation-sensitizer for therapy. Cleavage of X allows separation of A from B, unmasking the normal ability of the basic amino acids in B to drag cargo C into cells near the cleavage event. X is cleaved extracellularly, preferably under physiological conditions. D-amino acids are preferred for the A and B portions, to minimize immunogenicity and nonspecific cleavage by background peptidases or proteases.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {10}
}
Works referenced in this record:
Molecular Classification of Cancer: Class Discovery and Class Prediction by Gene Expression Monitoring
journal, October 1999
- Golub, T. R.
- Science, Vol. 286, Issue 5439, p. 531-537
Contraluminal para-aminohippurate (PAH) transport in the proximal tubule of the rat kidney: VI. Specificity: Amino acids, their N-methyl-, N-acetyl- and N-benzoylderivatives; glutathione- and cysteine conjugates, di- and oligopeptides
journal, December 1989
- Ullrich, K. J.; Rumrich, G.; Wieland, Th.
- Pfl�gers Archiv European Journal of Physiology, Vol. 415, Issue 3