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Title: Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate

Abstract

Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.

Inventors:
 [1];  [2];  [2];  [2]
  1. Oak Ridge, TN
  2. Knoxville, TN
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
985165
Patent Number(s):
7419575
Application Number:
10/858,096
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Culbertson, Christopher T, Jacobson, Stephen C, McClain, Maxine A, and Ramsey, J Michael. Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate. United States: N. p., 2008. Web.
Culbertson, Christopher T, Jacobson, Stephen C, McClain, Maxine A, & Ramsey, J Michael. Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate. United States.
Culbertson, Christopher T, Jacobson, Stephen C, McClain, Maxine A, and Ramsey, J Michael. Tue . "Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate". United States. https://www.osti.gov/servlets/purl/985165.
@article{osti_985165,
title = {Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate},
author = {Culbertson, Christopher T and Jacobson, Stephen C and McClain, Maxine A and Ramsey, J Michael},
abstractNote = {Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {9}
}

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Works referenced in this record:

Continuous Cell Introduction for the Analysis of Individual Cells by Capillary Electrophoresis
journal, January 2001


Electroosmotically Induced Hydraulic Pumping with Integrated Electrodes on Microfluidic Devices
journal, August 2001


Characterization of Cellular Optoporation with Distance
journal, March 2000


Effects of high electric fields on micro-organisms
journal, August 1968


Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects
journal, April 1997


Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects
journal, April 1997


Voltage-induced pore formation and hemolysis of human erythrocytes
journal, December 1977


Hemolysis of human erythrocytes by transient electric field.
journal, May 1977


Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane)
journal, October 1998