Methods for producing secreted polypeptides
Abstract
The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3' end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.
- Inventors:
-
- Gold River, CA
- Davis, CA
- Issue Date:
- Research Org.:
- Novozymes, Inc. (Davis, CA)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 984491
- Patent Number(s):
- 7393664
- Application Number:
- 10/837,318
- Assignee:
- Novozymes, Inc. (Davis, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC36-98GO10337
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Maiyuran, Suchindra, Fidantsef, Ana, and Brody, Howard. Methods for producing secreted polypeptides. United States: N. p., 2008.
Web.
Maiyuran, Suchindra, Fidantsef, Ana, & Brody, Howard. Methods for producing secreted polypeptides. United States.
Maiyuran, Suchindra, Fidantsef, Ana, and Brody, Howard. Tue .
"Methods for producing secreted polypeptides". United States. https://www.osti.gov/servlets/purl/984491.
@article{osti_984491,
title = {Methods for producing secreted polypeptides},
author = {Maiyuran, Suchindra and Fidantsef, Ana and Brody, Howard},
abstractNote = {The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3' end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {7}
}
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