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Title: Methods for producing secreted polypeptides

Abstract

The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3' end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.

Inventors:
 [1];  [2];  [2]
  1. Gold River, CA
  2. Davis, CA
Issue Date:
Research Org.:
Novozymes, Inc. (Davis, CA)
Sponsoring Org.:
USDOE
OSTI Identifier:
984491
Patent Number(s):
7393664
Application Number:
10/837,318
Assignee:
Novozymes, Inc. (Davis, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC36-98GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Maiyuran, Suchindra, Fidantsef, Ana, and Brody, Howard. Methods for producing secreted polypeptides. United States: N. p., 2008. Web.
Maiyuran, Suchindra, Fidantsef, Ana, & Brody, Howard. Methods for producing secreted polypeptides. United States.
Maiyuran, Suchindra, Fidantsef, Ana, and Brody, Howard. Tue . "Methods for producing secreted polypeptides". United States. https://www.osti.gov/servlets/purl/984491.
@article{osti_984491,
title = {Methods for producing secreted polypeptides},
author = {Maiyuran, Suchindra and Fidantsef, Ana and Brody, Howard},
abstractNote = {The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3' end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {7}
}

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Works referenced in this record:

Characterization of the Trichoderma reesei cbh2 promoter
journal, February 1993


The Molecular Cloning of the Major Cellulase Gene from Trichoderma Reesei
journal, October 1983


Cloning of genes encoding alpha-L-arabinofuranosidase and beta-xylosidase from Trichoderma reesei by expression in Saccharomyces cerevisiae.
journal, January 1996


Different Inducibility of Expression of the Two Xylanase Genes xyn1 and xyn2 in Trichoderma reesei
journal, October 1996


A new method for predicting signal sequence cleavage sites
journal, January 1986