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Title: Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination

Abstract

Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.

Inventors:
; ; ;
Issue Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
984459
Patent Number(s):
7399619
Application Number:
11/137,850
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
DOE Contract Number:  
FG02-03ER46051
Resource Type:
Patent
Resource Relation:
Patent File Date: 2005 May 24
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Xie, Jianming, Wang, Lei, Wu, Ning, and Schultz, Peter G. Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination. United States: N. p., 2008. Web.
Xie, Jianming, Wang, Lei, Wu, Ning, & Schultz, Peter G. Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination. United States.
Xie, Jianming, Wang, Lei, Wu, Ning, and Schultz, Peter G. Tue . "Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination". United States. https://www.osti.gov/servlets/purl/984459.
@article{osti_984459,
title = {Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination},
author = {Xie, Jianming and Wang, Lei and Wu, Ning and Schultz, Peter G.},
abstractNote = {Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {7}
}

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