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Title: Quantum dot conjugates in a sub-micrometer fluidic channel

Abstract

A nanofluidic channel fabricated in fused silica with an approximately 500 nm square cross section was used to isolate, detect and identify individual quantum dot conjugates. The channel enables the rapid detection of every fluorescent entity in solution. A laser of selected wavelength was used to excite multiple species of quantum dots and organic molecules, and the emission spectra were resolved without significant signal rejection. Quantum dots were then conjugated with organic molecules and detected to demonstrate efficient multicolor detection. PCH was used to analyze coincident detection and to characterize the degree of binding. The use of a small fluidic channel to detect quantum dots as fluorescent labels was shown to be an efficient technique for multiplexed single molecule studies. Detection of single molecule binding events has a variety of applications including high throughput immunoassays.

Inventors:
 [1];  [2];  [1];  [1]
  1. Ithaca, NY
  2. Brookline, MA
Issue Date:
Research Org.:
Cornell Univ., Ithaca, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
984446
Patent Number(s):
7405434
Application Number:
11/280,941
Assignee:
Cornell Research Foundation, Inc. (Ithaca, NY)
Patent Classifications (CPCs):
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
DOE Contract Number:  
FG02-99ER62809
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats

Stavis, Samuel M, Edel, Joshua B, Samiee, Kevan T, and Craighead, Harold G. Quantum dot conjugates in a sub-micrometer fluidic channel. United States: N. p., 2008. Web.
Stavis, Samuel M, Edel, Joshua B, Samiee, Kevan T, & Craighead, Harold G. Quantum dot conjugates in a sub-micrometer fluidic channel. United States.
Stavis, Samuel M, Edel, Joshua B, Samiee, Kevan T, and Craighead, Harold G. Tue . "Quantum dot conjugates in a sub-micrometer fluidic channel". United States. https://www.osti.gov/servlets/purl/984446.
@article{osti_984446,
title = {Quantum dot conjugates in a sub-micrometer fluidic channel},
author = {Stavis, Samuel M and Edel, Joshua B and Samiee, Kevan T and Craighead, Harold G},
abstractNote = {A nanofluidic channel fabricated in fused silica with an approximately 500 nm square cross section was used to isolate, detect and identify individual quantum dot conjugates. The channel enables the rapid detection of every fluorescent entity in solution. A laser of selected wavelength was used to excite multiple species of quantum dots and organic molecules, and the emission spectra were resolved without significant signal rejection. Quantum dots were then conjugated with organic molecules and detected to demonstrate efficient multicolor detection. PCH was used to analyze coincident detection and to characterize the degree of binding. The use of a small fluidic channel to detect quantum dots as fluorescent labels was shown to be an efficient technique for multiplexed single molecule studies. Detection of single molecule binding events has a variety of applications including high throughput immunoassays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {7}
}

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