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Title: Arrays of probes for positional sequencing by hybridization

Abstract

This invention is directed to methods and reagents useful for sequencing nucleic acid targets utilizing sequencing by hybridization technology comprising probes, arrays of probes and methods whereby sequence information is obtained rapidly and efficiently in discrete packages. That information can be used for the detection, identification, purification and complete or partial sequencing of a particular target nucleic acid. When coupled with a ligation step, these methods can be performed under a single set of hybridization conditions. The invention also relates to the replication of probe arrays and methods for making and replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5'- and/or 3'-overhangs.

Inventors:
 [1];  [2];  [1];  [3]
  1. Boston, MA
  2. East Boston, MA
  3. Waltham, MA
Issue Date:
Research Org.:
Boston University (Boston, MA)
Sponsoring Org.:
USDOE
OSTI Identifier:
983886
Patent Number(s):
7319003
Application Number:
09/030,571
Assignee:
The Trustees of Boston University (Boston, MA)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-93ER61609
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Cantor, Charles R, Prezetakiewiczr, Marek, Smith, Cassandra L, and Sano, Takeshi. Arrays of probes for positional sequencing by hybridization. United States: N. p., 2008. Web.
Cantor, Charles R, Prezetakiewiczr, Marek, Smith, Cassandra L, & Sano, Takeshi. Arrays of probes for positional sequencing by hybridization. United States.
Cantor, Charles R, Prezetakiewiczr, Marek, Smith, Cassandra L, and Sano, Takeshi. Tue . "Arrays of probes for positional sequencing by hybridization". United States. https://www.osti.gov/servlets/purl/983886.
@article{osti_983886,
title = {Arrays of probes for positional sequencing by hybridization},
author = {Cantor, Charles R and Prezetakiewiczr, Marek and Smith, Cassandra L and Sano, Takeshi},
abstractNote = {This invention is directed to methods and reagents useful for sequencing nucleic acid targets utilizing sequencing by hybridization technology comprising probes, arrays of probes and methods whereby sequence information is obtained rapidly and efficiently in discrete packages. That information can be used for the detection, identification, purification and complete or partial sequencing of a particular target nucleic acid. When coupled with a ligation step, these methods can be performed under a single set of hybridization conditions. The invention also relates to the replication of probe arrays and methods for making and replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5'- and/or 3'-overhangs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {1}
}

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