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Title: Amplification of trace amounts of nucleic acids

Abstract

Methods of reducing background during amplification of small amounts of nucleic acids employ careful analysis of sources of low level contamination. Ultraviolet light can be used to reduce nucleic acid contaminants in reagents and equipment. "Primer-dimer" background can be reduced by judicious design of primers. We have shown clean signal-to-noise with as little as starting material as one single human cell (.about.6 picogram), E. coli cell (.about.5 femtogram) or Prochlorococcus cell (.about.3 femtogram).

Inventors:
 [1];  [2]
  1. Brookline, MA
  2. Brighton, MA
Issue Date:
Research Org.:
Harvard University
Sponsoring Org.:
USDOE
OSTI Identifier:
983051
Patent Number(s):
7387876
Application Number:
11/066,559
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Church, George M, and Zhang, Kun. Amplification of trace amounts of nucleic acids. United States: N. p., 2008. Web.
Church, George M, & Zhang, Kun. Amplification of trace amounts of nucleic acids. United States.
Church, George M, and Zhang, Kun. Tue . "Amplification of trace amounts of nucleic acids". United States. https://www.osti.gov/servlets/purl/983051.
@article{osti_983051,
title = {Amplification of trace amounts of nucleic acids},
author = {Church, George M and Zhang, Kun},
abstractNote = {Methods of reducing background during amplification of small amounts of nucleic acids employ careful analysis of sources of low level contamination. Ultraviolet light can be used to reduce nucleic acid contaminants in reagents and equipment. "Primer-dimer" background can be reduced by judicious design of primers. We have shown clean signal-to-noise with as little as starting material as one single human cell (.about.6 picogram), E. coli cell (.about.5 femtogram) or Prochlorococcus cell (.about.3 femtogram).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {6}
}

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Works referenced in this record:

Whole-genome amplification of DNA from residual cells left by incidental contact
journal, January 2004


Comprehensive human genome amplification using multiple displacement amplification
journal, April 2002