Amplification of trace amounts of nucleic acids
Abstract
Methods of reducing background during amplification of small amounts of nucleic acids employ careful analysis of sources of low level contamination. Ultraviolet light can be used to reduce nucleic acid contaminants in reagents and equipment. "Primer-dimer" background can be reduced by judicious design of primers. We have shown clean signal-to-noise with as little as starting material as one single human cell (.about.6 picogram), E. coli cell (.about.5 femtogram) or Prochlorococcus cell (.about.3 femtogram).
- Inventors:
-
- Brookline, MA
- Brighton, MA
- Issue Date:
- Research Org.:
- Harvard University
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 983051
- Patent Number(s):
- 7387876
- Application Number:
- 11/066,559
- Assignee:
- President and Fellows of Harvard College (Cambridge, MA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-02ER63445
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Church, George M, and Zhang, Kun. Amplification of trace amounts of nucleic acids. United States: N. p., 2008.
Web.
Church, George M, & Zhang, Kun. Amplification of trace amounts of nucleic acids. United States.
Church, George M, and Zhang, Kun. Tue .
"Amplification of trace amounts of nucleic acids". United States. https://www.osti.gov/servlets/purl/983051.
@article{osti_983051,
title = {Amplification of trace amounts of nucleic acids},
author = {Church, George M and Zhang, Kun},
abstractNote = {Methods of reducing background during amplification of small amounts of nucleic acids employ careful analysis of sources of low level contamination. Ultraviolet light can be used to reduce nucleic acid contaminants in reagents and equipment. "Primer-dimer" background can be reduced by judicious design of primers. We have shown clean signal-to-noise with as little as starting material as one single human cell (.about.6 picogram), E. coli cell (.about.5 femtogram) or Prochlorococcus cell (.about.3 femtogram).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {6}
}
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journal, April 2002
- Dean, F. B.; Hosono, S.; Fang, L.
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