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Title: Cellobiohydrolase I gene and improved variants

Abstract

The disclosure provides a method for preparing an active exoglucanase in a heterologous host of eukaryotic origin. The method includes mutagenesis to reduce glycosylation of the exoglucanase when expressed in a heterologous host. It is further disclosed a method to produce variant cellobiohydrolase that is stable at high temperature through mutagenesis.

Inventors:
 [1];  [2];  [3];  [1];  [4];  [5];  [1]
  1. Golden, CO
  2. Berthoud, CO
  3. San Carlos, CA
  4. Lakewood, CO
  5. Littleton, CO
Issue Date:
Research Org.:
National Energy Technology Laboratory (NETL), Pittsburgh, PA, Morgantown, WV (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
982999
Patent Number(s):
7375197
Application Number:
10/031,496
Assignee:
MidWest Research Institute (Kansas City, MO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
DOE Contract Number:  
AC36-99G0-10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Adney, William S, Decker, Stephen R, Mc Carter, Suzanne, Baker, John O, Nieves, Raphael, Himmel, Michael E, and Vinzant, Todd B. Cellobiohydrolase I gene and improved variants. United States: N. p., 2008. Web.
Adney, William S, Decker, Stephen R, Mc Carter, Suzanne, Baker, John O, Nieves, Raphael, Himmel, Michael E, & Vinzant, Todd B. Cellobiohydrolase I gene and improved variants. United States.
Adney, William S, Decker, Stephen R, Mc Carter, Suzanne, Baker, John O, Nieves, Raphael, Himmel, Michael E, and Vinzant, Todd B. Tue . "Cellobiohydrolase I gene and improved variants". United States. https://www.osti.gov/servlets/purl/982999.
@article{osti_982999,
title = {Cellobiohydrolase I gene and improved variants},
author = {Adney, William S and Decker, Stephen R and Mc Carter, Suzanne and Baker, John O and Nieves, Raphael and Himmel, Michael E and Vinzant, Todd B},
abstractNote = {The disclosure provides a method for preparing an active exoglucanase in a heterologous host of eukaryotic origin. The method includes mutagenesis to reduce glycosylation of the exoglucanase when expressed in a heterologous host. It is further disclosed a method to produce variant cellobiohydrolase that is stable at high temperature through mutagenesis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {5}
}

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