Title: High temperature flow-through device for rapid solubilization and analysis

Abstract

Devices and methods for thermally lysing of biological material, for example vegetative bacterial cells and bacterial spores, are provided. Hot solution methods for solubilizing bacterial spores are described. Systems for direct analysis are disclosed including thermal lysers coupled to sample preparation stations. Integrated systems capable of performing sample lysis, labeling and protein fingerprint analysis of biological material, for example, vegetative bacterial cells, bacterial spores and viruses are provided.

Inventors:

West, Jason A. A. ^[1]; Hukari, Kyle W ^[2]; Patel, Kamlesh D ^[3]; Peterson, Kenneth A ^[4]; Renzi, Ronald F ^[5]

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+ Show Inventor Affiliations

1. Castro Valley, CA
2. San Ramon, CA
3. Dublin, CA
4. Albuquerque, NM
5. Tracy, CA

Issue Date:

2009-09-22

Research Org.:

Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

971614

Patent Number(s):

7592139

Application Number:

11/059,079

Assignee:

Sandia National Laboratories (Liverpool, CA)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2300/0877 - Flow chambers
B01L2300/0887 - Laminated structure
B01L2300/1827 - using resistive heater
B01L3/5027 - {by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
B01L7/00 - Heating or cooling apparatus

C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY

C12M47/06 - {Hydrolysis
C12M47/20 - {Heating or cooling

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N1/066 - {by physical methods}

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DOE Contract Number:  

AC04-94AL85000

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES