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Title: Method for converting sucrose to .beta.-D-glucose

Abstract

Disclosed is an apparatus and method for continuously converting sucrose to .beta.-D-glucose. The method comprises a three-stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and .alpha.-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.

Inventors:
 [1];  [2];  [3];  [4]
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  1. San Francisco, CA
  2. Livermore, CA
  3. Albuquerque, NM
  4. Woodinville, WA
Issue Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
968969
Patent Number(s):
7556945
Application Number:
11/825,615
Assignee:
Sandia Corporation (Livermore, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
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DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Simmons, Blake A, Volponi, Joanne V, Ingersoll, David, and Walker, Andrew. Method for converting sucrose to .beta.-D-glucose. United States: N. p., 2009. Web.
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Simmons, Blake A, Volponi, Joanne V, Ingersoll, David, & Walker, Andrew. Method for converting sucrose to .beta.-D-glucose. United States.
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Simmons, Blake A, Volponi, Joanne V, Ingersoll, David, and Walker, Andrew. Tue . "Method for converting sucrose to .beta.-D-glucose". United States. https://www.osti.gov/servlets/purl/968969.
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@article{osti_968969,
title = {Method for converting sucrose to .beta.-D-glucose},
author = {Simmons, Blake A and Volponi, Joanne V and Ingersoll, David and Walker, Andrew},
abstractNote = {Disclosed is an apparatus and method for continuously converting sucrose to .beta.-D-glucose. The method comprises a three-stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and .alpha.-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {7}
}
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