Method for converting sucrose to .beta.-D-glucose
Abstract
Disclosed is an apparatus and method for continuously converting sucrose to .beta.-D-glucose. The method comprises a three-stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and .alpha.-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.
- Inventors:
-
- San Francisco, CA
- Livermore, CA
- Albuquerque, NM
- Woodinville, WA
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 968969
- Patent Number(s):
- 7556945
- Application Number:
- 11/825,615
- Assignee:
- Sandia Corporation (Livermore, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Simmons, Blake A, Volponi, Joanne V, Ingersoll, David, and Walker, Andrew. Method for converting sucrose to .beta.-D-glucose. United States: N. p., 2009.
Web.
Simmons, Blake A, Volponi, Joanne V, Ingersoll, David, & Walker, Andrew. Method for converting sucrose to .beta.-D-glucose. United States.
Simmons, Blake A, Volponi, Joanne V, Ingersoll, David, and Walker, Andrew. Tue .
"Method for converting sucrose to .beta.-D-glucose". United States. https://www.osti.gov/servlets/purl/968969.
@article{osti_968969,
title = {Method for converting sucrose to .beta.-D-glucose},
author = {Simmons, Blake A and Volponi, Joanne V and Ingersoll, David and Walker, Andrew},
abstractNote = {Disclosed is an apparatus and method for continuously converting sucrose to .beta.-D-glucose. The method comprises a three-stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and .alpha.-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {7}
}
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