Method for dialysis on microchips using thin porous polymer membrane
Abstract
Laser-induced phase-separation polymerization of a porous acrylate polymer is used for in-situ fabrication of dialysis membranes inside glass microchannels. A shaped 355 nm laser beam is used to produce a porous polymer membrane with a thickness of about 15 .mu.m, which bonds to the glass microchannel and forms a semi-permeable membrane. Differential permeation through a membrane formed with pentaerythritol triacrylate was observed and quantified by comparing the response of the membrane to fluorescein and fluorescently tagging 200 nm latex microspheres. Differential permeation was observed and quantified by comparing the response to rhodamine 560 and lactalbumin protein in a membrane formed with SPE-methylene bisacrylamide. The porous membranes illustrate the capability for the present technique to integrate sample cleanup into chip-based analysis systems.
- Inventors:
-
- San Francisco, CA
- Livermore, CA
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 963814
- Patent Number(s):
- 7534315
- Application Number:
- 11/825,600
- Assignee:
- Sandia Corporation (Livermore, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Singh, Anup K, Kirby, Brian J, and Shepodd, Timothy J. Method for dialysis on microchips using thin porous polymer membrane. United States: N. p., 2009.
Web.
Singh, Anup K, Kirby, Brian J, & Shepodd, Timothy J. Method for dialysis on microchips using thin porous polymer membrane. United States.
Singh, Anup K, Kirby, Brian J, and Shepodd, Timothy J. Tue .
"Method for dialysis on microchips using thin porous polymer membrane". United States. https://www.osti.gov/servlets/purl/963814.
@article{osti_963814,
title = {Method for dialysis on microchips using thin porous polymer membrane},
author = {Singh, Anup K and Kirby, Brian J and Shepodd, Timothy J},
abstractNote = {Laser-induced phase-separation polymerization of a porous acrylate polymer is used for in-situ fabrication of dialysis membranes inside glass microchannels. A shaped 355 nm laser beam is used to produce a porous polymer membrane with a thickness of about 15 .mu.m, which bonds to the glass microchannel and forms a semi-permeable membrane. Differential permeation through a membrane formed with pentaerythritol triacrylate was observed and quantified by comparing the response of the membrane to fluorescein and fluorescently tagging 200 nm latex microspheres. Differential permeation was observed and quantified by comparing the response to rhodamine 560 and lactalbumin protein in a membrane formed with SPE-methylene bisacrylamide. The porous membranes illustrate the capability for the present technique to integrate sample cleanup into chip-based analysis systems.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 19 00:00:00 EDT 2009},
month = {Tue May 19 00:00:00 EDT 2009}
}