Protein detection system

Fruetel, Julie A; Fiechtner, Gregory J; Kliner, Dahv A. V.; McIlroy, Andrew

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A - human necessities
A01 - agriculture
A21 - baking
A22 - butchering
A23 - foods or foodstuffs
A24 - tobacco
A41 - wearing apparel
A42 - headwear
A43 - footwear
A44 - haberdashery
A45 - hand or travelling articles
A46 - brushware
A47 - furniture
A61 - medical or veterinary science
A62 - life-saving
A63 - sports
A99 - subject matter not otherwise provided for in this section
B - performing operations
B01 - physical or chemical processes or apparatus in general
B02 - crushing, pulverising, or disintegrating
B03 - separation of solid materials using liquids or using pneumatic tables or jigs
B04 - centrifugal apparatus or machines for carrying-out physical or chemical processes
B05 - spraying or atomising in general
B06 - generating or transmitting mechanical vibrations in general
B07 - separating solids from solids
B08 - cleaning
B09 - disposal of solid waste
B21 - mechanical metal-working without essentially removing material
B22 - casting
B23 - machine tools
B24 - grinding
B25 - hand tools
B26 - hand cutting tools
B27 - working or preserving wood or similar material
B28 - working cement, clay, or stone
B29 - working of plastics
B30 - presses
B31 - making articles of paper, cardboard or material worked in a manner analogous to paper
B32 - layered products
B33 - additive manufacturing technology
B41 - printing
B42 - bookbinding
B43 - writing or drawing implements
B44 - decorative arts
B60 - vehicles in general
B61 - railways
B62 - land vehicles for travelling otherwise than on rails
B63 - ships or other waterborne vessels
B64 - aircraft
B65 - conveying
B66 - hoisting
B67 - opening, closing {or cleaning} bottles, jars or similar containers
B68 - saddlery
B81 - microstructural technology
B82 - nanotechnology
B99 - subject matter not otherwise provided for in this section
C - chemistry
C01 - inorganic chemistry
C02 - treatment of water, waste water, sewage, or sludge
C03 - glass
C04 - cements
C05 - fertilisers
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C07 - organic chemistry
C08 - organic macromolecular compounds
C09 - dyes
C10 - petroleum, gas or coke industries
C11 - animal or vegetable oils, fats, fatty substances or waxes
C12 - biochemistry
C13 - sugar industry
C14 - skins
C21 - metallurgy of iron
C22 - metallurgy
C23 - coating metallic material
C25 - electrolytic or electrophoretic processes
C30 - crystal growth
C40 - combinatorial technology
C99 - subject matter not otherwise provided for in this section
D - textiles
D01 - natural or man-made threads or fibres
D02 - yarns
D03 - weaving
D04 - braiding
D05 - sewing
D06 - treatment of textiles or the like
D07 - ropes
D10 - indexing scheme associated with sublasses of section d, relating to textiles
D21 - paper-making
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E - fixed constructions
E01 - construction of roads, railways, or bridges
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F - mechanical engineering
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F22 - steam generation
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G - physics
G01 - measuring
G02 - optics
G03 - photography
G04 - horology
G05 - controlling
G06 - computing
G07 - checking-devices
G08 - signalling
G09 - education
G10 - musical instruments
G11 - information storage
G12 - instrument details
G16 - information and communication technology [ict] specially adapted for specific application fields
G21 - nuclear physics
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H - electricity
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Y02 - technologies or applications for mitigation or adaptation against climate change
Y04 - information or communication technologies having an impact on other technology areas
Y10 - technical subjects covered by former uspc

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Title: Protein detection system

Abstract

The present embodiment describes a miniature, microfluidic, absorption-based sensor to detect proteins at sensitivities comparable to LIF but without the need for tagging. This instrument utilizes fiber-based evanescent-field cavity-ringdown spectroscopy, in combination with faceted prism microchannels. The combination of these techniques will increase the effective absorption path length by a factor of 10.sup.3 to 10.sup.4 (to .about.1-m), thereby providing unprecedented sensitivity using direct absorption. The coupling of high-sensitivity absorption with high-performance microfluidic separation will enable real-time sensing of biological agents in aqueous samples (including aerosol collector fluids) and will provide a general method with spectral fingerprint capability for detecting specific bio-agents.

Inventors:

Fruetel, Julie A ^[1]; Fiechtner, Gregory J ^[2]; Kliner, Dahv A. V. ^[3]; McIlroy, Andrew ^[1]

Livermore, CA
Bethesda, MD
San Ramon, CA

Issue Date:: Tue May 05 00:00:00 EDT 2009

Research Org.:: Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 963807

Patent Number(s):: 7527977

Application Number:: 11/087,942

Assignee:: Sandia Corporation (Livermore, CA)

Patent Classifications (CPCs):: B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2200/10 - Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
B01L2300/0645 - Electrodes
B01L2300/0654 - Lenses
B01L3/502715 - {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2021/0346 - {Capillary cells
G01N2021/391 - {Intracavity sample}
G01N21/359 - using near infra-red light
G01N21/552 - Attenuated total reflection
G01N21/7703 - {using reagent-clad optical fibres or optical waveguides
G01N21/7746 - {the waveguide coupled to a cavity resonator}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
Y10T436/2575 - Volumetric liquid transfer

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DOE Contract Number:: AC04-94AL85000

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 47 OTHER INSTRUMENTATION

Citation Formats


                    Fruetel, Julie A, Fiechtner, Gregory J, Kliner, Dahv A. V., and McIlroy, Andrew. Protein detection system.  United States: N. p., 2009. 
        Web.

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                    Fruetel, Julie A, Fiechtner, Gregory J, Kliner, Dahv A. V., & McIlroy, Andrew. Protein detection system.  United States.

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                    Fruetel, Julie A, Fiechtner, Gregory J, Kliner, Dahv A. V., and McIlroy, Andrew. Tue .  
        "Protein detection system".  United States.  https://www.osti.gov/servlets/purl/963807.

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@article{osti_963807,

  title        = {Protein detection system},

  author       = {Fruetel, Julie A and Fiechtner, Gregory J and Kliner, Dahv A. V. and McIlroy, Andrew},

  abstractNote = {The present embodiment describes a miniature, microfluidic, absorption-based sensor to detect proteins at sensitivities comparable to LIF but without the need for tagging. This instrument utilizes fiber-based evanescent-field cavity-ringdown spectroscopy, in combination with faceted prism microchannels. The combination of these techniques will increase the effective absorption path length by a factor of 10.sup.3 to 10.sup.4 (to .about.1-m), thereby providing unprecedented sensitivity using direct absorption. The coupling of high-sensitivity absorption with high-performance microfluidic separation will enable real-time sensing of biological agents in aqueous samples (including aerosol collector fluids) and will provide a general method with spectral fingerprint capability for detecting specific bio-agents.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue May 05 00:00:00 EDT 2009},

  month        = {Tue May 05 00:00:00 EDT 2009}

}

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Works referenced in this record:

Direct Monitoring of Absorption in Solution by Cavity Ring-Down Spectroscopy
journal, April 2002

Hallock, Alexander J.; Berman, Elena S. F.; Zare, Richard N.
Analytical Chemistry, Vol. 74, Issue 7
https://doi.org/10.1021/ac011103i

Cavity ring-down spectroscopy in the liquid phase
journal, February 2002

Xu, Shucheng; Sha, Guohe; Xie, Jinchun
Review of Scientific Instruments, Vol. 73, Issue 2
https://doi.org/10.1063/1.1430729

Cavity ring down spectroscopy on solid C60
journal, February 1999

Engeln, Richard; von Helden, Gert; van Roij, André J. A.
The Journal of Chemical Physics, Vol. 110, Issue 5
https://doi.org/10.1063/1.477997

Evanescent wave cavity ring-down spectroscopy with a total-internal-reflection minicavity
journal, August 1997

Pipino, Andrew C. R.; Hudgens, Jeffrey W.; Huie, Robert E.
Review of Scientific Instruments, Vol. 68, Issue 8, p. 2978-2989
https://doi.org/10.1063/1.1148230

Gene probe assays on a fibre-optic evanescent wave biosensor
journal, January 1992

Graham, C. R.; Leslie, D.; Squirrell, D. J.
Biosensors and Bioelectronics, Vol. 7, Issue 7
https://doi.org/10.1016/0956-5663(92)80005-V

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Similar Records

Title: Protein detection system

Abstract

Citation Formats

Direct Monitoring of Absorption in Solution by Cavity Ring-Down Spectroscopy journal, April 2002

Cavity ring-down spectroscopy in the liquid phase journal, February 2002

Cavity ring down spectroscopy on solid C60 journal, February 1999

Evanescent wave cavity ring-down spectroscopy with a total-internal-reflection minicavity journal, August 1997

Gene probe assays on a fibre-optic evanescent wave biosensor journal, January 1992

Direct Monitoring of Absorption in Solution by Cavity Ring-Down Spectroscopy
journal, April 2002

Cavity ring-down spectroscopy in the liquid phase
journal, February 2002

Cavity ring down spectroscopy on solid C60
journal, February 1999

Evanescent wave cavity ring-down spectroscopy with a total-internal-reflection minicavity
journal, August 1997

Gene probe assays on a fibre-optic evanescent wave biosensor
journal, January 1992