Protein detection system

Fruetel, Julie A; Fiechtner, Gregory J; Kliner, Dahv A. V.; McIlroy, Andrew

Title: Protein detection system

Abstract

The present embodiment describes a miniature, microfluidic, absorption-based sensor to detect proteins at sensitivities comparable to LIF but without the need for tagging. This instrument utilizes fiber-based evanescent-field cavity-ringdown spectroscopy, in combination with faceted prism microchannels. The combination of these techniques will increase the effective absorption path length by a factor of 10.sup.3 to 10.sup.4 (to .about.1-m), thereby providing unprecedented sensitivity using direct absorption. The coupling of high-sensitivity absorption with high-performance microfluidic separation will enable real-time sensing of biological agents in aqueous samples (including aerosol collector fluids) and will provide a general method with spectral fingerprint capability for detecting specific bio-agents.

Inventors:

Fruetel, Julie A ^[1]; Fiechtner, Gregory J ^[2]; Kliner, Dahv A. V. ^[3]; McIlroy, Andrew ^[1]

Livermore, CA
Bethesda, MD
San Ramon, CA

Issue Date:: 2009-05-05

Research Org.:: Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 963807

Patent Number(s):: 7527977

Application Number:: 11/087,942

Assignee:: Sandia Corporation (Livermore, CA)

Patent Classifications (CPCs):: B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2300/0645 - Electrodes

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N21/7746 - {the waveguide coupled to a cavity resonator}
G01N2021/0346 - {Capillary cells

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2300/0654 - Lenses

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N21/7703 - {using reagent-clad optical fibres or optical waveguides

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
Y10T436/2575 - Volumetric liquid transfer

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2021/391 - {Intracavity sample}
G01N21/552 - Attenuated total reflection
G01N21/359 - using near infra-red light

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L3/502715 - {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
B01L2200/10 - Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept

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DOE Contract Number:: AC04-94AL85000

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 47 OTHER INSTRUMENTATION

Citation Formats


                    Fruetel, Julie A, Fiechtner, Gregory J, Kliner, Dahv A. V., and McIlroy, Andrew. Protein detection system.  United States: N. p., 2009. 
        Web.

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                    Fruetel, Julie A, Fiechtner, Gregory J, Kliner, Dahv A. V., & McIlroy, Andrew. Protein detection system.  United States.

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                    Fruetel, Julie A, Fiechtner, Gregory J, Kliner, Dahv A. V., and McIlroy, Andrew. Tue .  
        "Protein detection system".  United States.  https://www.osti.gov/servlets/purl/963807.

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@article{osti_963807,

  title        = {Protein detection system},

  author       = {Fruetel, Julie A and Fiechtner, Gregory J and Kliner, Dahv A. V. and McIlroy, Andrew},

  abstractNote = {The present embodiment describes a miniature, microfluidic, absorption-based sensor to detect proteins at sensitivities comparable to LIF but without the need for tagging. This instrument utilizes fiber-based evanescent-field cavity-ringdown spectroscopy, in combination with faceted prism microchannels. The combination of these techniques will increase the effective absorption path length by a factor of 10.sup.3 to 10.sup.4 (to .about.1-m), thereby providing unprecedented sensitivity using direct absorption. The coupling of high-sensitivity absorption with high-performance microfluidic separation will enable real-time sensing of biological agents in aqueous samples (including aerosol collector fluids) and will provide a general method with spectral fingerprint capability for detecting specific bio-agents.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2009},

  month        = {5}

}

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Works referenced in this record:

Direct Monitoring of Absorption in Solution by Cavity Ring-Down Spectroscopy
journal, April 2002

Hallock, Alexander J.; Berman, Elena S. F.; Zare, Richard N.
Analytical Chemistry, Vol. 74, Issue 7
https://doi.org/10.1021/ac011103i

Cavity ring-down spectroscopy in the liquid phase
journal, February 2002

Xu, Shucheng; Sha, Guohe; Xie, Jinchun
Review of Scientific Instruments, Vol. 73, Issue 2
https://doi.org/10.1063/1.1430729

Cavity ring down spectroscopy on solid C60
journal, February 1999

Engeln, Richard; von Helden, Gert; van Roij, André J. A.
The Journal of Chemical Physics, Vol. 110, Issue 5
https://doi.org/10.1063/1.477997

Evanescent wave cavity ring-down spectroscopy with a total-internal-reflection minicavity
journal, August 1997

Pipino, Andrew C. R.; Hudgens, Jeffrey W.; Huie, Robert E.
Review of Scientific Instruments, Vol. 68, Issue 8, p. 2978-2989
https://doi.org/10.1063/1.1148230

Gene probe assays on a fibre-optic evanescent wave biosensor
journal, January 1992

Graham, C. R.; Leslie, D.; Squirrell, D. J.
Biosensors and Bioelectronics, Vol. 7, Issue 7
https://doi.org/10.1016/0956-5663(92)80005-V

Gene probe assays on a fibre-optic evanescent wave biosensor
journal, January 1992

Graham, C. R.; Leslie, D.; Squirrell, D. J.
Biosensors and Bioelectronics, Vol. 7, Issue 7
https://doi.org/10.1016/0956-5663(92)80005-V

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Title: Protein detection system

Abstract

Citation Formats

Direct Monitoring of Absorption in Solution by Cavity Ring-Down Spectroscopy journal, April 2002

Cavity ring-down spectroscopy in the liquid phase journal, February 2002

Cavity ring down spectroscopy on solid C60 journal, February 1999

Evanescent wave cavity ring-down spectroscopy with a total-internal-reflection minicavity journal, August 1997

Gene probe assays on a fibre-optic evanescent wave biosensor journal, January 1992

Gene probe assays on a fibre-optic evanescent wave biosensor journal, January 1992

Direct Monitoring of Absorption in Solution by Cavity Ring-Down Spectroscopy
journal, April 2002

Cavity ring-down spectroscopy in the liquid phase
journal, February 2002

Cavity ring down spectroscopy on solid C60
journal, February 1999

Evanescent wave cavity ring-down spectroscopy with a total-internal-reflection minicavity
journal, August 1997

Gene probe assays on a fibre-optic evanescent wave biosensor
journal, January 1992

Gene probe assays on a fibre-optic evanescent wave biosensor
journal, January 1992