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Title: Protein detection system

Abstract

The present embodiment describes a miniature, microfluidic, absorption-based sensor to detect proteins at sensitivities comparable to LIF but without the need for tagging. This instrument utilizes fiber-based evanescent-field cavity-ringdown spectroscopy, in combination with faceted prism microchannels. The combination of these techniques will increase the effective absorption path length by a factor of 10.sup.3 to 10.sup.4 (to .about.1-m), thereby providing unprecedented sensitivity using direct absorption. The coupling of high-sensitivity absorption with high-performance microfluidic separation will enable real-time sensing of biological agents in aqueous samples (including aerosol collector fluids) and will provide a general method with spectral fingerprint capability for detecting specific bio-agents.

Inventors:
 [1];  [2];  [3];  [1]
  1. Livermore, CA
  2. Bethesda, MD
  3. San Ramon, CA
Issue Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
963807
Patent Number(s):
7527977
Application Number:
11/087,942
Assignee:
Sandia Corporation (Livermore, CA)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Fruetel, Julie A, Fiechtner, Gregory J, Kliner, Dahv A. V., and McIlroy, Andrew. Protein detection system. United States: N. p., 2009. Web.
Fruetel, Julie A, Fiechtner, Gregory J, Kliner, Dahv A. V., & McIlroy, Andrew. Protein detection system. United States.
Fruetel, Julie A, Fiechtner, Gregory J, Kliner, Dahv A. V., and McIlroy, Andrew. Tue . "Protein detection system". United States. https://www.osti.gov/servlets/purl/963807.
@article{osti_963807,
title = {Protein detection system},
author = {Fruetel, Julie A and Fiechtner, Gregory J and Kliner, Dahv A. V. and McIlroy, Andrew},
abstractNote = {The present embodiment describes a miniature, microfluidic, absorption-based sensor to detect proteins at sensitivities comparable to LIF but without the need for tagging. This instrument utilizes fiber-based evanescent-field cavity-ringdown spectroscopy, in combination with faceted prism microchannels. The combination of these techniques will increase the effective absorption path length by a factor of 10.sup.3 to 10.sup.4 (to .about.1-m), thereby providing unprecedented sensitivity using direct absorption. The coupling of high-sensitivity absorption with high-performance microfluidic separation will enable real-time sensing of biological agents in aqueous samples (including aerosol collector fluids) and will provide a general method with spectral fingerprint capability for detecting specific bio-agents.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {5}
}

Works referenced in this record:

Direct Monitoring of Absorption in Solution by Cavity Ring-Down Spectroscopy
journal, April 2002


Cavity ring-down spectroscopy in the liquid phase
journal, February 2002


Cavity ring down spectroscopy on solid C60
journal, February 1999


Evanescent wave cavity ring-down spectroscopy with a total-internal-reflection minicavity
journal, August 1997


Gene probe assays on a fibre-optic evanescent wave biosensor
journal, January 1992


Gene probe assays on a fibre-optic evanescent wave biosensor
journal, January 1992