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Title: Nucleotide sequences specific to Brucella and methods for the detection of Brucella

Abstract

Nucleotide sequences specific to Brucella that serves as a marker or signature for identification of this bacterium were identified. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

Inventors:
 [1];  [2];  [3];  [4];  [4];  [4]
  1. Tracy, CA
  2. San Mateo, CA
  3. Berkeley, CA
  4. Livermore, CA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
961574
Patent Number(s):
7494773
Application Number:
10/630,607
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-7405-ENG-48; AC52-07NA27344
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

McCready, Paula M, Radnedge, Lyndsay, Andersen, Gary L, Ott, Linda L, Slezak, Thomas R, and Kuczmarski, Thomas A. Nucleotide sequences specific to Brucella and methods for the detection of Brucella. United States: N. p., 2009. Web.
McCready, Paula M, Radnedge, Lyndsay, Andersen, Gary L, Ott, Linda L, Slezak, Thomas R, & Kuczmarski, Thomas A. Nucleotide sequences specific to Brucella and methods for the detection of Brucella. United States.
McCready, Paula M, Radnedge, Lyndsay, Andersen, Gary L, Ott, Linda L, Slezak, Thomas R, and Kuczmarski, Thomas A. Tue . "Nucleotide sequences specific to Brucella and methods for the detection of Brucella". United States. https://www.osti.gov/servlets/purl/961574.
@article{osti_961574,
title = {Nucleotide sequences specific to Brucella and methods for the detection of Brucella},
author = {McCready, Paula M and Radnedge, Lyndsay and Andersen, Gary L and Ott, Linda L and Slezak, Thomas R and Kuczmarski, Thomas A},
abstractNote = {Nucleotide sequences specific to Brucella that serves as a marker or signature for identification of this bacterium were identified. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {2}
}

Works referenced in this record:

Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification
journal, September 1996


Specificity of a Polymerase Chain Reaction Assay of a Target Sequence on the 31-Kilodalton Brucella Antigen DNA Used to Diagnose Human Brucellosis
journal, March 2001

  • Casañas, M. C.; Queipo-Ortuño, M. I.; Rodriguez-Torres, A.
  • European Journal of Clinical Microbiology and Infectious Diseases, Vol. 20, Issue 2
  • https://doi.org/10.1007/PL00011242

Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes.
journal, January 1996


Serum Is the Preferred Clinical Specimen for Diagnosis of Human Brucellosis by PCR
journal, April 2001