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Title: Synthesis of DNA

Abstract

A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.

Inventors:
 [1]
  1. (Danville, CA)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
957000
Patent Number(s):
7452666
Application Number:
10/394,911
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Mariella, Jr., Raymond P. Synthesis of DNA. United States: N. p., 2008. Web.
Mariella, Jr., Raymond P. Synthesis of DNA. United States.
Mariella, Jr., Raymond P. Tue . "Synthesis of DNA". United States. https://www.osti.gov/servlets/purl/957000.
@article{osti_957000,
title = {Synthesis of DNA},
author = {Mariella, Jr., Raymond P.},
abstractNote = {A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {11}
}

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Works referenced in this record:

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides
journal, October 1995